LOCUS       sequence-31799-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, 
            Lieberman J
  TITLE     miR-34a contributes to megakaryocytic differentiation of K562 cells 
            independently of p53.
  JOURNAL   Blood. 2009 Sep 3. 114(10):2181-92.
  PUBMED    19584398
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2009
            Sep 3. 114(10):2181-92."
COMMENT     Sequence Label: pLL3.7_hsa-miR-34a
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        119..367
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     119..139
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     290..309
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     635..651
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     636..652
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(694..727)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
       61 gacagcagag atccagtttg gttagtaccg ggcccgctct agaagatctg ggcaggaaga
      121 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
      181 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
      241 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      301 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      361 aggatccccc acatttcctt cttatcaaca ggtgctgggg agaggcagga caggcctgtc
      421 ccccgagtcc cctccggatg ccgtggaccg gccagctgtg agtgtttctt tggcagtgtc
      481 ttagctggtt gttgtgagca atagtaagga agcaatcagc aagtatactg ccctagaagt
      541 gctgcacgtt gtggggccca agagggaaga tgaagcgaga gatgcccaga ccagtgggag
      601 acgccaggac ttcggaagct cttctgcgaa ttcctcgagg tcgacggtat cgataagctc
      661 gcttcacgag attccagcag gtcgagggac ctaataactt cgtatagcat acattatacg
      721 aagttatatt aagggttcca agcttaagcg gccgcgtgga taaccgtatt accgccatgc
      781 attagttatt aatagtaatc aattac
//
