LOCUS       sequence-317992-       18898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Gateway compatible pDEST vector to clone transgene 
            downstream of smu-1 for germline expression from arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18898)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 18898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 gen...
FEATURES             Location/Qualifiers
     source          1..18898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(149..160)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2226..2246
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3242..3266
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11510..11609)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        11766..11868
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11869..12552
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(11935..11954)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             12872..13177
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     13056..13075
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(13567..13690)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(13698..13715)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13698..13714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15541..15563
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(15601..15619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15687..15791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15792..15860,15861..16652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16010..16029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16823..17411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17312..17331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgagcca atttatccaa gtccttgtaa aaagtattcg aaaattgtta acggataaaa
       61 tgtttattat aatatcaaaa acaatttgtc agttgaccac tttttgatat atttgacaga
      121 aaccgggatg aattggctca aagttagggc gcccttctat tacagggttt ctgataacaa
      181 accggttatt aactcccaac aagggatgat ttcaattcat catgctcaaa ttgacccaaa
      241 ttaagttaca tgacaaattc atcgcccttt tcaactcttc ttggctcatc atctgttatt
      301 ctgttcctat tctgtctgca ccctataccc ctttgcatac tctctcgtca ttcctttctt
      361 ggatagtgtc ttcttctccc cagctctgct acttctatga cttgcgcccg ctgcttttcc
      421 gctcgttcct ctcctctctg acgtcttctt ctgcttcttt tctccttcct ttttcccttc
      481 tcaatcttcc tctttttccc atttctgtca atcattcgaa gaagaagaag aagaagaccc
      541 tcattcattt catttttttt tctgtcggtg tgtgctgctc ggttaagagt gagctctcta
      601 ttccacgtct tcttcttttt cttcttcgat tcgaatcaat cactccacca aaacgcattc
      661 gtttttggga ttcaccccgc ggttcgcaat aggtttcttt ttcaaattat tagcgttata
      721 aatagaaaaa tggtggagtt tcaataaaaa tgataattta caaagtgtat tttgattaca
      781 tgtactcaaa aaggttgaaa tttttcatac cagttttccg gaaatccatc tgatatcatt
      841 atcgtatttt tctttttaaa aatgttttca aaaaaaaaca aaatatagct ggttatttgg
      901 caccctctaa ttaccatttt cctgtcacac cacactcttt tccttcttcc acttctttcc
      961 gttttcagcc gcttccaacc aaaccgatat gaaagccgag caacaacgat cgattccacc
     1021 cggctcggcg accttcccgt cgcaggtgag actcagaaaa ctagagaaac cgctcaacta
     1081 actcttgata tccgatttca ttcttttctc ttttcttttt gttgaacttt tccatatttc
     1141 cagatgccac ggccaccacc aagcaccgaa caaggaatca caacggaatc ggagcttgcg
     1201 aagaaagctc aaatcactcc gaacgacgtt ttagcacttc cgggaatcac tcaaggtatc
     1261 ttgctccctt tggatttcag tttaaaacat aaatttagga ttcttatgct ccccatctgc
     1321 gaacatctat aacatcgagt tcaccaagtt ccaaatccgt gatctggaca ctgagcaagt
     1381 gcttttcgag atcgccaaac cggagaacga tcaggagaat gatgagtcgc cacaggagtc
     1441 ggcaagatac gtgcgttata gatttgctcc aaactttttg aaactcaaaa cggtcggagc
     1501 aactgtggaa ttcaaagtag gagacatccc aatccatcat ttccgaatga tcgaacgtca
     1561 cttcttcaaa gatcgccttc tgaagtgttt tgactttgaa ttcggattct gtattccgaa
     1621 ttcacgaaac aactgtgaac atatctatga gttccctcaa ctctctcaac aactcagtga
     1681 gtcattattc taaaaagtac aattcaaaag actaatctct ttttcagtgg acgacatgat
     1741 caacaatcca aacgagactc gttctgacag cttctatttc gtcgataaca aactcgtcat
     1801 gcacaacaaa gccgactact catatgatgc ataaatattt aatacaaaaa tgttctggat
     1861 aattattctg tcgaatagaa aaaaaactcc aaatgtgatt aaattccaat aattcctgtc
     1921 tagtttgttc cttccccttc cccttctcat gttcaatgca ttcctaagct tttcagttcc
     1981 ccccttgttt ctatattttt tcgctgtcct gtcacactcg ctaaaaacac taatcacacg
     2041 gaaatctgtt ttcaataaaa actccaactt taactcattt tcaatttcaa ctgaaagatt
     2101 ttttcattag agaatgtcta gaactaggcc cgggctacgt aatacgactc acttaaggcc
     2161 ttgactagag ggtaccagag ctcacctagg caggaacagc tatgaccatg attacgccaa
     2221 gctatcaact ttgtatagaa aagttgaaat ttttcaggcg ggaagtggct tcggtgcgtt
     2281 caacaaacgc gcttttgact cgctagccgg atccggcttc agcggttttg acaaaagagc
     2341 tttcgattca ctggctggac aaggttttac tggatttgag aaacgcgcct ttgatacagt
     2401 ggtacggttt tcggtggaaa ttccagtttt tcgaattttc aataattttc agagcacgtc
     2461 aggctttgac gatttcaaat tgtgaagagc tcactgatat ttaaaatttg gttcaattca
     2521 agcacatatt tttgaggtaa gcaactgatt ttaatgttgt taatcaaatg aaaagtgaaa
     2581 tttttggaat aatttttttt tgttttcagg tagctccaac gaacgacaca cacaagccca
     2641 gtcccccagt attcctgacg acagcccctg ctcacacact ttttattata attccttttt
     2701 tttcaaactt ttttttcaaa ttttatactt taatctcaaa aaacctattc atacgaacac
     2761 aagtaccggt gtgcgtgtgt gagaataaga atttatttta aaaaaataaa gaatgtcgtc
     2821 ctgttttttc atatttattg aagaaaatgg agctggaaaa aaatcaattg taaaaaacag
     2881 aaaaacgtgc tgataaaatg cattgtacgc aaactgtgga ttttgagaac taacgtagtc
     2941 aagcaaaaat ctctggaatt ttatcgattt ttaacggata aactgttgtt ttttgtaact
     3001 gtgcatgaaa aaaaatctca ggcaaaaata ttttctgaaa aaaaaagagt ttaaacacaa
     3061 atgcaagtcc acaaaaaact ccaatttgaa attcgctcca aaaacgcaaa acacgccaag
     3121 cgctcgtgct ggcgcatttg ccgatcgctt ccaaaaaggc tggcgcactt ctcgtgtccg
     3181 aggatattcg aattttacac gtcattttca gccgtttttc accgaatttt cgctgaaaat
     3241 gacaagtttg tacaaaaaag caggctcgtc atcgattgag atcgaatctt cagagtaagt
     3301 ttatctaaaa agtttttcat tcaaaatgtg taaaaattca tttaaaataa cccaaaaatc
     3361 attaatcctc gatattttca gtgtaatccg tctcatcgaa caattcctca aagaatcgaa
     3421 tttgcaccga actctggcga ttttgcaggt attttcctgc atttttcaac tgggaaaatg
     3481 aaagaaaatc gataatttca ggaagaaacc aacgtttcgc tcaatacagt cgactcaatt
     3541 gatggatttt gtaatgaaat tacgtctgga aattgggata acgtgttgaa aaccgttcaa
     3601 tcgttgaaat tacccgccaa gaaacttatc gatctgtatg aacatgtaag atatgggaaa
     3661 gaaggaaaaa accgagattt tacttgaaaa attgaatttt tcgcgggatt ttcaccaaaa
     3721 attgttgaat attcattatt tcacgctgta aaacaaaaaa aaaaaaaatc aaaaactacg
     3781 ttgaaatcgc gtttttaagc gaattttctt cagaattgcc agattttaac cccaaatttt
     3841 gcagttttta aataaaattt caccttttcg gctcaaattg tagattttcc tgaaaattta
     3901 gtacaaaaaa caatttcctc gtaaattttt caaattttca ggtaataatc gagcttgtgg
     3961 agctccgtga gctggcgacc gcccgtctcg tcgcccgtca gactgatcca atgattttgc
     4021 taaaacaaat tgatccggat cgattcgccc gcctggaatc tcttatcaac cgcccgtatt
     4081 tcgatggaca agaggtttat ggagatgtta gtaaagagaa acgacgctcc gtaattgcac
     4141 aaactctttc atcagaagtt catgtagttg ctccgtcaag acttttgagt ttacttggac
     4201 aatcacttaa atggcaactt catcaaggac ttcttccacc tggaactgca attgatttat
     4261 ttagaggaaa agctgcacag aaagagcaaa ttgaagaaag atatccgact atgatggcta
     4321 gaagcattaa attctcaacg agtacggatt gaaattgctt taaaatttga aaaattgatt
     4381 aaaaagtgca ttttttaagc tttgaccgac ttaaaattag atttctgagc ctattttctg
     4441 agaattggaa tttttttcat tgtgaaagtt caaagaatag cgtgaatgat tagaaaatat
     4501 tgtaaaattt caattttttc ctataaaaaa ggattttttt aggaatcaaa aattgcaaaa
     4561 tgatgcccta aaattcgaaa ataaatataa aaattggcgg tttctcaaaa atctagaatt
     4621 ccgaccttaa ttattaattt ttttacaata tttttttttg aaaaaaatcc agaaaatttg
     4681 aatttcgtag tttgtagtct aggcctctat caattaaatt ttcgattttt tgagtaaaat
     4741 ttcgaattta ctattatttg gaccaaaatt gtattttttt ttcagaatta aatttataaa
     4801 attttcagaa attaaattta taaaaaaaaa aaaaaattaa aaaaaaaaaa aaaaattaaa
     4861 aattaaaaat taaaaaatta agtttttttt actcaaaatt ttgcactgaa attcgaaaat
     4921 ctaaaaatcc gacctaaagt ctgtattttt tcaacaaaaa ttcagaaaaa actcaaaaac
     4981 ttgtattttg tagccagtca accactttct aaaatatcaa atttgaattt ttcaagcatt
     5041 tttacattga aaaatctaat ttttcgagtg aaaccacttg aaaatctgaa aattgaatta
     5101 atttctgaac tttagagatt ttttgtcaaa attttagata aagggatttt taaacaaaaa
     5161 attgattttt taatcgaaaa atactgggat ttatgggttt tttaaaagaa aaacgggggt
     5221 ttgaatgaaa aatcgcctga aatcttagaa aattaattaa aaaactatga tttaattcca
     5281 aaaattaccg aaaatatcaa attttccatt tttaaaccct aaattctttc agaaagttat
     5341 cccgaatcgg cagttttctc accggacgct aattatcttg tttctggctc aaaagacgga
     5401 tttatcgaag tttggaatta tatgaatgga aaattgagaa aggatttgaa atatcaggta
     5461 gattttttag aatttttggg tttttgaagt agaaaatcat aaaaatctag ggtttttatg
     5521 aattgttttg aagaaaaatt gcaaaaattc cacaaaatgg aagaaaaata actttggaag
     5581 cgcatttttc gcaaaaaaac cgaaattttt gcgtaaaatt tcaaaattgc aataaaaatt
     5641 ccacaaacat caaatttctt aaatttttta taaaaaattg gatggaaaca ctcttgaatt
     5701 tagaaaaaaa atcagttttt ctcatctaaa acttcaaaat ttcggtgtaa tcccattaaa
     5761 attgccacaa aattcggaaa tttcacctga aatagagtga ataatttaaa atgttcagaa
     5821 attcatattt tgtcatttta aaagcattaa aacaaatcaa aaatctattt tttttggttg
     5881 gaaaagttca aaattctgga gaaattacat taaaattttc gtaaaatatg gtaaataaac
     5941 gaaaatgttg agaaattaaa gaaaagttac aatttttagc ttaaaaattc aacattttga
     6001 ggaaatgcca cctaaaaaag tgactaatcg aaaatgttga gaaattaaaa ttgccaccat
     6061 ttatttatat aaactactct aaaattacaa ttttcatgtt aaaaattaat aaaaaatcta
     6121 ctttttccaa actacagtaa ccttaccgta tacctacagt acctgaacat tgccccccac
     6181 cagctcccaa cccaatacct cctcaaaaac ttacacctca atttttcata aactacagta
     6241 accctaccaa aaaagcacaa aaaaaaatct acattcattt tccaacaatt ttcaatattt
     6301 tcaggcccaa gataatctaa tgatgatgga tgcagcagtg agatgtatat catttagtcg
     6361 tgattctgaa atgctcgcca caggctcaat tgacggaaaa attaaagttt ggaaagtgga
     6421 aactggtgat tgtcttcgaa gatttgatag agctcacacg aaaggagttt gtgctgttcg
     6481 attctcaaaa gataattcac atattttgtc gggtggaaat gatcatgttg tgaggtacga
     6541 ctacctgcct gcctaccgcc taaatttttt gtgaagtttc ttcaaaaaat ccagaaaaaa
     6601 aaacaatttt catacgattt tttcccttaa aattgtgaat tttcatgctt tttagcccca
     6661 aaagtcatta tttgagaaaa aatttcatac aaaaaagttt tgagaaatac acaatttttt
     6721 aaatgtaatt ttcaaatttt caattttcaa ctagaaaatt cacaaaactt gtaaattttg
     6781 gaccaaaaca tttatacaat tacttttttt gaatctaata actacaataa ctacaaattt
     6841 tgttcagagt tcatggaatg aagagcggaa aatgtttgaa ggaaatgcgt gggcactcga
     6901 gttatattac agatgtcaga tattccgatg agggaaatca tattgtgagt ttttgaaaag
     6961 tgcagtttgt agtctaattt tcatttattt tctttttaaa aaacgcatca atttttaaat
     7021 attttgggac aaaatccgaa aactgtacta atttgtagtt tgtaaaatta aaaaaaaaac
     7081 gcaaaaaaat gttttcaaaa atgcttagaa taaaaataaa atttctaaaa aattgaacaa
     7141 aataaacatt taaaaattca aaaagtttga aaaaatgcaa agttttttaa tgcaaaaaat
     7201 atttaatgtt taaacaaatt taaaaaatgt tgtaagaata tgtttagaga cagtttttaa
     7261 atgttaaatt gcaaaaaaaa aaagcaaaaa aaaatttgaa aaatgtaaca gaaaatttta
     7321 attaaaaaag tcgtgttttt ttcaaacctg caattctttg taattttatt tataaagaga
     7381 aacaaatcaa attgtatatt acgaaaaacg atactaaaat tcgaaaattt gcggtttttt
     7441 gcgtaaaaaa tacggttcct gaaaatgggt gtgcaccttt aaggagtact gtagttctaa
     7501 actttcattg ctgcaaaatt tatatctatt ttttgaagtt ttacaataaa aataacaatt
     7561 ttcattaaat aactacaatt aaacagctaa aaacatataa gaaatcgcga gaaaatcggg
     7621 aaaaaattgt agtttgtagt atgttagccg caatcaaatg catgttatct ctgtatttct
     7681 tttctcacac aaatcccaca tatttctatg tttacacacg tcattttgtg cactttttct
     7741 gacgtttccc ctccatattg attttgcttt ttactgttta gaaacgttgc agaaatccgg
     7801 aaaaattgat tttttttcta tatatatata tttttttcca agtttcaaat ttaaatttca
     7861 gaaaaaaaat ttagacttaa aaaaattaaa aatcccaaat aatttccaga tctcatgttc
     7921 aacagatggt tcaattcgtg tttggcatgg aaaatcgggt gaatgccttt caacattccg
     7981 tgttggaagt gaagattatc caatattaaa tgtgattcca attccgaaaa gtgatccacc
     8041 acaaatgatt gtatgcaatc gatccaatac cctctacgtt gttaatattt ctggacaggt
     8101 aacatgattt tgaaaaaaag tttaagaact gaaaaatgga ataaaaatat ttaagagcat
     8161 ttttaaatgt aaaattaaca aaaaaagcgc ctaagaatgt ttccaaaaca gtaaaaaaaa
     8221 ggtttaaaaa aatgcaaaaa aaaatttaag atgcttttaa ttacacaaaa aatgaaagta
     8281 aaaaaaatta aaatattgaa aaatgtatat ttgtttgaaa aggtacattt ttcaatgcaa
     8341 aaaatgtttc taaaaattaa aaaaatatgt ttaaattgtg taaagtgtaa attaaaaaat
     8401 aaaattatga aaaagtattg tttcatactt tttctaaatt ttggttgaaa aatttaaaat
     8461 tccagctttt tattcacatt atgtgttcta aaaatctttc cccaattttc gggaccgttt
     8521 tccagtatat ttccattttt caaaaagatt tttaactgaa attcatgttt tcaatgctaa
     8581 aaatcaataa aaaaaatttc aggtggttcg tacgatgacg tcaggaaagc gtgagaaagg
     8641 agacttcatc aactgcattt tatcgccgaa aggttaattt tgacccgaaa attgaaaaaa
     8701 aaaaccgatt tcaaaattct aattttccca tttttttctt taaaataatt tctcctctgg
     8761 aaaggctcgt agagcagaaa aagaattaaa ttagaaacgg atttttgttc aaatttcccc
     8821 cgttttcccc ttcaaaactt gcaattcagt ttcttacagg agaatgggca tacgcaattg
     8881 cagaggacgg tgtcatgtac tgttttatgg tattatcggg taccctggaa actactcttc
     8941 ccgtaagttt agcctctttt tgaactcaaa ttttagttta aaaagcgaga aaaagtacaa
     9001 ggaaaatgag aaatcaagtg catgaattga taagaatagt atggtgaggg tgtggttgat
     9061 gactagaaaa aatcgttttt ttttaatcat acacagattt tcttaaaata aaaacatcaa
     9121 aaatgtgtcg gactaatttt gtaaaataaa atttaatcga aaaatgaggt aaagttgaat
     9181 tttttgactc caaaaacctc tatttctacg ctcaattttg taaaaaaaaa ttcagggtaa
     9241 tttttatttc aatgtctaca aattttgggg gtattgctac tgaaaaagta ccgaaaaatt
     9301 atttttttcg caaaaaaccg ttatttctga aaaaattctg aaatttattc ttcaaaatgt
     9361 taaaaatacg cagaatattt cagtaccaaa aaactaaaaa aaaaaacaaa atatcacaaa
     9421 aaaacgaaat aaatcgaatt gcgaatttcc ccaactctcg ttagattccc tatttgaatt
     9481 ccttccaaaa tgaaaactcc ccgtccaatc agcgtcttcg aacactccgc ccactcattc
     9541 tgattggttc aataatggac ggctatagaa aatctataga aaatcacttg atttatctga
     9601 aaccaacatt aatttcagct gaaaaattag agaaagggct caaaattggc ctgaaagttg
     9661 ccaattttta ttaaaaaaat tggcaacttt caggcaaatt tttatatatt ttaaaattct
     9721 aattagtcct catttcgata ttccacgttt ccgaattttg atattttttt ttcaaaatat
     9781 atattcctca ttaaaaatgt tctagttttt tttacaacat ccctcttgcc tccaaaattt
     9841 ttcgattttc tcccattcct ccctcactcg gaagaatgtt cttcgactga cgtagtggta
     9901 gtagcagccc ataacacata cgtagattaa ttttatagat agtatagggt tttcctacac
     9961 cgatattcat tccattgttc acttttcgaa ttgtttcata ttgcgatttt aaaggcgcat
    10021 ggcttgaaaa gtatgaagtt tagaaatttt acagaaaaaa cgaaaaaaaa atccgaaaaa
    10081 accagaattt cctatctcct ccctcactaa cctcaataaa ttcccagttt gaattccctc
    10141 catattgtga actgcaaaac caatcagcga tttgctctgc ccacttttca accaatcaga
    10201 taaagtgggc ggggttcgaa gtctctgggg gcgtgttacg gagctaccta tttcatcatg
    10261 ctcttcccag acgtgggcaa cggttgccat tcggcaattt tttggtcgac aagttcggca
    10321 aatctgcaaa ttgccggttt gtcgatttgc cggaaacagc cgcccacccc tggttcctcc
    10381 aacatttttc attaaaaatt tgtgtaaaac ttcagaaaat ctctcgactt tcctttaaaa
    10441 agaaacacta atttcagctg aaacactcga aaatggccta aaagttggaa attgccgatt
    10501 ttcagaccat ttttgagcat tttctctaag atttttgtag tttgtagtct ttctgccaat
    10561 ttgtttaatt cagtcttccc ctctctccaa ttaaacaatt cccgttcaca tatttttttc
    10621 aatatgaccg cactcggtac acttttctgt ctgcatctct actctctctc tctctctctc
    10681 acggaaaatt catttttttt gttgtttttc tcttccgtgg tccgtctaga agatgctagg
    10741 aatactaaat atatatattt cgtcaatttt ttttccgccc gcccgtctct cgcccaacgt
    10801 gctaggcaac cggctttttt ttgttgaaaa ttttgaagat ttcgatgaaa aatgtagatt
    10861 ttttttcggg tttctgagct gaaaatcact gataaagctc acaactttat cagaaaatgt
    10921 ttgatttttt tttcaaattt tgggtcccgc cacgatcaac ctgatgacgg atactgtagt
    10981 cttgaaaatt aggttttatt gaatttttag gaaaagttta aatattcagc tgaaattcga
    11041 aaattgcagt ctgagccaca atcaattttt tttcaaaagc gccccattct tggatcttgc
    11101 aacgaagatt ttttaggtta ctgtagaaac aaaaaattgt aggttgtagt catgaatatt
    11161 tttcgacttt ttcaaaataa ataagttcaa ataaaatgga aattcagcga aaaaaaagta
    11221 aatttttatg agaaaaatga agaaaattta aaaaaaaaca aaaaaatttt ttcgctataa
    11281 aaaaaattag aaacgaatat tttcaagaaa attcaataat tttgattgaa aattggagaa
    11341 aaaagttcca gttttttgct atttttctta ttttttattg aaaaaaaaat ttaaaaatcg
    11401 ataattttcc aggtgacaga acgtcttcca attggtttgg ctcatcatcc acatcagaat
    11461 ctcatcgctt catatgcaga agatggactt ttaaaattat ggaccgataa cccagctttc
    11521 ttgtacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
    11581 actatcagtc aaaataaaat cattatttgg agctctagag cgtcgactaa gttggcagca
    11641 tcacccgacg cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa
    11701 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
    11761 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
    11821 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
    11881 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
    11941 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
    12001 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
    12061 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
    12121 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
    12181 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
    12241 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
    12301 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
    12361 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
    12421 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
    12481 cttaatgaat tacaacagta ctgcgatgag tggcaggggg ggcgtaaacg ccgcgtggat
    12541 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa
    12601 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg
    12661 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
    12721 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
    12781 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
    12841 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag
    12901 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
    12961 acggatggtg atccccctgg ccagtgcacg tctgctgtcg gataaagtct cccgtgaact
    13021 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
    13081 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
    13141 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
    13201 ccagtctgca ggtcgataca gtagaaatta cagaaacttt atcacgttta gtaagtatag
    13261 aggctgaaaa tccagatgaa gccgaacgac ttgtaagaga aaagtataag agttgtgaaa
    13321 ttgttcttga tgcagatgat tttcaggact atgacactag cgtatatgaa taggtagatg
    13381 tttttatttt gtcacacaaa aaagaggctc gcacctcttt tccttatttc tttttatgat
    13441 ttaatacggc attgaggaca atagcgagta ggctggatac gacgattccg tttgagaaga
    13501 acatttggaa ggctgtcggt cgagctcgaa ttctacaggt cactaatacc atctaagtag
    13561 ttgattcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
    13621 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aactttatta
    13681 tacatagttg ataattcact ggccgtcgtt ttacactcga gacgtacggt gcgcgcgatg
    13741 cattcgaaga tctgcccact agtgagtcgt attataagtg caagtaagat cagtgtttgt
    13801 ttcgatatca gtctgtttcg taacggtctt ctgtataact acaaaaaaga ataaaaaacc
    13861 gtatcacaac tgagaaaaaa gacaaaactt attgaacagt gttcctttca aaaaattatt
    13921 ttgtaaaaaa tcgagttttt ctccaaaata acgcacgcaa tcaaaaacaa gaaaactaac
    13981 gtgttctcct gggcgtggaa ctccttatca agattaccat tattgtctgt cagaattgat
    14041 atttcaatta tttcatatct agacaaaccc gggtgtcttg catttattag tactgtgagt
    14101 ttacatacaa acgaggctta ctcagataac atagagtcac ctctaattag caaaaaccca
    14161 tttctaatta gctatttcga ccagttctaa agtcaccgta attagaaatg gtttttgtag
    14221 cttttctaat tagaaatgac cccgagataa gtgaaatatg gcattttctg ggttttctag
    14281 taagaaatga tcccgcacgt agtgattccg cgttacaagc caaattcaaa ttaaaaaagt
    14341 atattacaca attcaagtta cattattttc aggaaacaat ggcgaagagc aaagcagaga
    14401 ttcaaaagga ggaaagagac aacagatatg aacgaagact tcttgaaaag agaaagagac
    14461 atgaggtgag taaccatgat aataaataat caagtaaggg ctatagattt ataaattctg
    14521 gagttaacag tttcgaaaaa aagtagtttt catatgtgcg aaggaatatc aaaaataggg
    14581 gaatagccta aacgagtgag aaaattaaat gtttgttatt ttttcaaaaa tgtctgcagg
    14641 gccgttatcc tttcaaatgc actctatcaa acatagatca tatccatcat atcttatcca
    14701 gaatttatat catatccaag attgagagcg tttcaaggaa aatctcaaac aaccatacaa
    14761 ttttgtagat cgacaacaag gcctccactt cttcatcgat ggcctctgca gcacctgcac
    14821 caccaagttt gtatttttca tattaatata aagtgagcca cgcatagtcg tttttaggat
    14881 ttttgccaga tcgatgtcgc gcaaacagac tgtttttttt tctttgaaag ccgtgcgtga
    14941 catgggccac ggattgtaac gccgcgctgg atggataaaa atttattcag ttttttagcc
    15001 tttacaaaat atggttcgac tactttcttt atttttacat ttttataggt gctcattgag
    15061 ctgagatatt ttgggtataa tcggaaaatc gctgattttg gtcatttttc tgcactaaag
    15121 attacacaaa aagttcagta tttagattca ttgttgaaaa ttggggatga tctacagata
    15181 catatcgccg tgttcaaaaa cacacgtgct gtgacgtcgt gactggggaa aaccctggcg
    15241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    15301 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
    15361 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    15421 gtgcaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    15481 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    15541 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
    15601 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    15661 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    15721 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    15781 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    15841 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    15901 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    15961 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    16021 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    16081 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    16141 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    16201 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    16261 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    16321 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    16381 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    16441 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    16501 agcgtgggtc tcgcggtatc attgcagcac tggggtcaga tggtaagccc tcccgtatcg
    16561 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    16621 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    16681 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    16741 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    16801 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    16861 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    16921 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    16981 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    17041 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    17101 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    17161 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    17221 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    17281 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    17341 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    17401 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt
    17461 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    17521 ttgagtgagc tgataccagc ggataacaat ttcacagggc cctgtcggat tatgggctct
    17581 tcttttatcc tctactttct ttctagaaaa tgcaaatgtt gagtcagaaa ttgctgaatg
    17641 gagtacaact gcaatgggtt tagcttatgt ttctggaaca attactggtg gaattgtgat
    17701 tgaaaagtaa ggttatattt catttcaata aatgttacca cagtataaca aaaacaagtt
    17761 acattgttca gagtatgtct gaatgtatta ttattattgt atttgtgcat cagtactgta
    17821 gttgtactga agaagggttt aaaataatct caacgttttt aagacgagtt agtgagaata
    17881 cggtcgcagt taggatgttt ggacatagtt ttattagata atcgtgaact aactcactat
    17941 aaaaacccgc taactcattg aataagatag ttttaaacgt tcaaaatttg aagacaaaat
    18001 tgtatgaata agtttacaga gttggtcgac gcaagcttct ccttctcttc acatttctca
    18061 ataatttagc tctaattgca ttcgtattct tcgcaaaaat acgtattctg attgacccga
    18121 tgaaatacgg atgcctagtt gcattgatca tctacggtta tacttatggg tgaggttttt
    18181 caaatatatt ttgatttaat cgaataatga aatttcagaa ctggagttgg tccaatttcg
    18241 tggtttatat cctctgaact ggtacctcaa aaacatcgta gtattgctca atctgtggca
    18301 tattcaatga atactgtaat ggtggttatc agtacattta cagtattacc tctatattcc
    18361 gttattggta gttatgcttt ccttattcta tattcaattc cttcatttat aagtatgctt
    18421 atattatttc gctacttacc ggaaaccaaa ggacgagaga ttcatgatat tgtgaatgaa
    18481 cttaaaaaaa aatgaaaacc tttgaaaacg ttcatacggc cgaaatttta cttgatcgag
    18541 ccaattgaac ttttgttaag acttacagaa aagtgagaga ttttgttaca aaaaagtgag
    18601 aaaaaagcga aaatgaacag gaaaaagaag aaaaatacac ccgaagtatc atataaaaat
    18661 taaaaagatt gtttgacctg gcggaacttt tttgcaaaaa ctctgaatat ccatggcaca
    18721 tgcttcgtgc aaaacaggat ccaaatgaat atcaacaaga gattcttgaa gacgacgagc
    18781 cacttgctca gagcattgct tatctcgaat gagacccttg ttaaaatcat ttttcaaaca
    18841 ttatatgaca ttgtcgaaat gtcctcctga ttccatgatg gtagcaaact cacttcgt
//
