LOCUS       sequence-318004-       13050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 24xgp41 peptide array (MoonTag peptide array), followed by
            kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide 
            array), linker and 24 repeats of PP7 hairpins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis 
            LD, Tanenbaum ME
  TITLE     Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
            in mRNA Decoding.
  JOURNAL   Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 
            10.1016/j.cell.2019.05.001.
  PUBMED    31178119
REFERENCE   2  (bases 1 to 13050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001."
COMMENT     Sequence Label: 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7...
FEATURES             Location/Qualifiers
     source          1..13050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1002..1046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1062..1106
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1122..1166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1182..1226
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1242..1286
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1302..1346
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1362..1406
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1422..1466
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1482..1526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1542..1586
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1602..1646
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1662..1706
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1782..1826
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1842..1886
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1902..1946
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1962..2006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2022..2066
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2082..2126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2142..2186
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2202..2246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2262..2306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2322..2366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2382..2426
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             5046..5102
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5118..5174
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5190..5246
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5262..5318
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5334..5390
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5406..5462
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5478..5534
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5550..5606
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5622..5678
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5694..5750
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5766..5822
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5838..5894
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5910..5966
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5982..6038
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6054..6110
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6126..6182
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6198..6254
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6270..6326
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6342..6398
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6414..6470
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6486..6542
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6558..6614
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6702..6758
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(9058..9075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9064..9288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9334..9762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9421..9440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9631..9652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9771..9791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9776..10105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9956..10091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10018..10037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10153..10200
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10219..10593
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10723..10844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10760..10779)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10814..10833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10893..10909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10893..10909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10906..10928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10917..10933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10941..10947,10948..10965,10966..10971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10986..11007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11124..11141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11295..11883)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11375..11394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12054..12845,12846..12914))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12677..12696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12915..13019)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgcgtacggg caagaatgag caggaactgc
     1021 tggaactgga caaatgggca agcctgggga gcggctccgg aaagaacgag caggaactgc
     1081 tggaactgga caagtgggct tctctgggct ccgggtccgg gaaaaacgaa caggagctgc
     1141 tggagctgga taagtgggcc agcctgggct ccgggtccgg gaagaatgaa caggaactgc
     1201 tggaactgga taaatgggca tctctgggca gcgggtccgg aaagaatgaa caggagctgc
     1261 tggaactgga caagtgggca tccctgggga gcgggtccgg aaagaatgaa caggaactgc
     1321 tggaactgga taagtgggca tccctgggaa gcggctccgg aaaaaacgag caggagctgc
     1381 tggagctgga caagtgggcc tctctgggct ccggctccgg gaagaatgaa caggagctgc
     1441 tggaactgga taagtgggca tctctggggt ccggaagcgg taaaaacgag caggaactgc
     1501 tggaactgga caagtgggca agcctggggt ctggctccgg caaaaacgaa caggaactgc
     1561 tggaactgga caaatgggcc agcctgggct ccgggtccgg gaaaaatgag caggaactgc
     1621 tggaactgga taaatgggcg agcctgggca gcgggtccgg aaaaaacgaa caggagctgc
     1681 tggagctgga caaatgggcg agtctgggct ccggaagcgg caagaatgaa caggaactgc
     1741 tggagctgga caaatgggcc agcctgggca gcggctccgg aaagaatgag caggaactgc
     1801 tggagctgga caaatgggca tctctgggct ccggctccgg gaaaaacgag caggaactgc
     1861 tggagctgga caagtgggct tccctgggca gcggctccgg aaaaaatgaa caggaactgc
     1921 tggaactgga caagtgggcc agcctggggt ccgggtctgg gaagaacgag caggaactgc
     1981 tggagctgga caagtgggca tctctggggt ctggctctgg caagaacgag caggaactgc
     2041 tggaactgga caagtgggcg agcctgggca gcgggtctgg caagaatgaa caggagctgc
     2101 tggaactgga caaatgggcc agcctggggt ctgggtctgg aaagaatgag caggaactgc
     2161 tggagctgga caagtgggca tctctgggct ctgggtctgg caagaacgag caggagctgc
     2221 tggagctgga taagtgggca tccctggggt ccgggtccgg caagaatgaa caggagctgc
     2281 tggagctgga taaatgggca tccctgggct ctgggtctgg gaagaatgag caggagctgc
     2341 tggaactgga taagtgggcg agcctgggca gcgggtctgg caagaatgag caggaactgc
     2401 tggagctgga caagtgggct tctctgcacc ggtcagcagt ggaggacagc acgctgcaag
     2461 tagtggtacg ggtgcggccc cccacccctc gggagctgga cagtcagcgg cggccagtgg
     2521 ttcaggtggt ggacgagcgg gtgctggtgt ttaaccctga ggagcccgat ggagggttcc
     2581 ctggcctgaa atggggtggc acccatgatg gccccaagaa gaagggcaaa gacctgacgt
     2641 ttgtctttga ccgggtcttt ggcgaggcgg ccacccaaca ggacgtgttc cagcacacca
     2701 cgcacagcgt cctggacagc ttcctccagg gctacaactg ctcagtgttt gcctacgggg
     2761 ccaccggggc tgggaagaca cacaccatgc tgggaaggga gggggacccc ggcatcatgt
     2821 acctgaccac cgtggaactg tacaggcgcc tggaggcccg ccagcaggag aagcacttcg
     2881 aggtgctcat cagctaccag gaggtgtata atgaacagat ccatgacctc ctggagccca
     2941 aggggcccct tgccatccgc gaggaccccg acaagggggt ggtggtgcaa ggactttctt
     3001 tccaccagcc agcctcagcc gagcagctgc tggagatact gaccaggggg aaccgtaacc
     3061 gcacgcagca ccccactgat gccaacgcga cttcctcccg ctcccatgcc atcttccaga
     3121 tctttgtgaa gcagcaggac cgggttccag gactgaccca ggctgtccag gtggccaaga
     3181 tgagcctgat tgacctggct ggctcagagc gggcatccag cacccatgcg aagggggagc
     3241 ggctgcggga gggggccaac atcaaccgct ctctgctggc gctcatcaac gtcctcaatg
     3301 ccttggccga tgcaaagggc cgcaagaccg ctgtgcccta cgcggacagc gcactgaccc
     3361 gcctgctcaa agactccctc gggggcaact gccgcacagt gatgatcgct gccatcagcc
     3421 cctccagcct gacctacgag gacacgtata ataccctcaa atatgccgac cgggccaagg
     3481 agatcaggct ctcgctgaag agcaatgtga ccagcctgga ctgtcacatc agccagtatg
     3541 ctaccatctg ccaacagctc caggctgagg tagccgctct gaggaagaag ctccaagtgt
     3601 atgagggggg aggccagccc ccaccacagg acctcccagg atctcccaag tcgggaccac
     3661 caccagaaca ccttcccagc tcccccttgc caccccaccc tcccagccag ccctgcaccc
     3721 cagagctccc tgcagggcct agagcccttc aagaggagag tctggggatg gaggcccagg
     3781 tggagagggc catggaaggg aactcttcag accaggagca gtccccagag gatgaggatg
     3841 aaggcccagc tgaggaggtt ccaacccaga tgccagagca gaaccccaca catgcactgc
     3901 cagagtcccc tcgcctgacc ctgcagccca agccagtcgt gggccacttc tcagcacggg
     3961 aactggatgg ggaccgttct aagcggttgg ccctaaaggt gctgtgcgtt gcccagcggc
     4021 agtactccct gctccaagca gccaacctcc tgacgcccga catgatcaca gagtttgaga
     4081 ccctacagca gctggtgcaa gaggaaaaaa ttgagcctgg ggcagaggcc ttgaggactt
     4141 caggcctggc caggggggca cctctggctc aggagctgtg ttcagagtca aagcctccag
     4201 gatacactgg ccctgtgacc cggactatgg cgaggcgact gagtggcccc ctgcacaccc
     4261 tgggaatccc gcctggaccc aactgcaccc cagcccaggg gtcccgatgg cccatggaga
     4321 agaagaggag gagaccaagc gccttggagg cagacagtcc catggcccca aagcggggca
     4381 ccaagcgcca gcgccagtcc ttcctgccct gcctaaggag agggtctctg cctgacaccc
     4441 aaccttcaca ggggcccagc acccccaaag gagaaagggc ctcctccccc tgccattccc
     4501 ctcgcgtttg cccagccaca gtcatcaaaa gccgggtgcc cctgggccct tccgccatgc
     4561 agaactgctc caccccgctg gctctgccca ctcgagacct caatgccacc tttgatctct
     4621 ctgaggagcc tccctcaaag cccagtttcc atgaatgcat tggctgggac aaaatacccc
     4681 aggagctgag caggctggac cagcccttca tccccagggc acctgtgccc ctgttcacca
     4741 tgaagggccc caagccaaca tcttccctcc ctgggacctc tgcctgcaag aagaagcgcg
     4801 ttgcgagttc ctcagtctcc catggccgca gccgcatcgc ccgcctcccc agcagcactt
     4861 tgaagaggcc agctgggccc cttgtactcc cagagctgcc cttgagtccc ctgtgcccta
     4921 gcaaccggag gaatggaaag gacctcatca gggtggggag agcactctca gcagggaacg
     4981 gcgtcaccaa ggtgtccgat atctgaatcg atggaggtgg atctggaggt ggaggttctg
     5041 gaggagaaga acttttgagc aagaattatc atcttgagaa cgaagtggct cgtcttaaga
     5101 aaggttctgg cagtggagaa gaactgcttt caaagaatta ccacctggaa aatgaggtag
     5161 ctagactgaa aaaggggagc ggaagtgggg aggagttgct gagcaaaaat tatcatttgg
     5221 agaacgaagt agcacgacta aagaaagggt ccggatcggg tgaggagtta ctctcgaaaa
     5281 attatcatct cgaaaacgaa gtggctcggc taaaaaaggg cagtggttct ggagaagagc
     5341 tattatctaa aaactaccac ctcgaaaatg aggtggcacg cttaaaaaag ggaagtggca
     5401 gtggtgaaga gctactatcc aagaattatc atcttgagaa cgaggtagcg cgtttgaaga
     5461 agggttccgg ctcaggagag gaactgctct cgaagaacta tcatcttgaa aatgaggtcg
     5521 ctcgattaaa aaagggatcg ggcagtggtg aggaactact ttcaaagaat taccacctcg
     5581 aaaacgaagt agctcgatta aagaaaggtt cagggtcggg tgaagaatta ctgagtaaaa
     5641 attatcatct ggaaaatgag gtagcgagac taaaaaaggg gagtggttct ggcgaagagt
     5701 tgctatcgaa aaattatcat cttgagaacg aagttgctag gctcaaaaag ggctcaggct
     5761 caggcgagga gttgctctcg aaaaactacc acttggaaaa tgaggtcgcg aggttgaaaa
     5821 aggggagcgg gtcgggcgag gagttattga gcaaaaacta tcatttagag aacgaagtcg
     5881 cgcgcttaaa gaaaggctcg ggctcgggcg aagaactctt atcgaagaac taccacctcg
     5941 aaaatgaggt cgccaggttg aaaaagggca gtggcagcgg ggaggaactc ttgagcaaga
     6001 actaccactt ggagaatgag gtcgcgagat tgaagaaagg gtcggggagc ggcgaggaat
     6061 tgctcagcaa gaattatcat ttggagaacg aagtcgccag gctcaagaaa ggctcggggt
     6121 cgggggagga attgttgagt aaaaactacc acttggaaaa tgaagtcgcc aggctcaaaa
     6181 aagggagtgg gagcggcgaa gagttattga gcaaaaatta ccacttggag aacgaagtgg
     6241 caaggctcaa gaaagggagc ggcagcgggg aggagctctt atcgaagaac taccacttag
     6301 agaatgaagt cgcccgcttg aagaaaggct cggggagcgg ggaagagctc ttgagcaaga
     6361 actaccactt ggaaaatgag gtggcgcgct tgaagaaagg gagcgggagc ggggaagagt
     6421 tactatctaa gaattatcat ctcgagaacg aggtggctcg actaaagaag ggctccggca
     6481 gtggggagga actcctgtcg aagaactatc atcttgaaaa tgaggttgca agacttaaaa
     6541 aggggtccgg atcaggtgag gaactactca gtaagaatta ccacctggaa aacgaagttg
     6601 cacgtttgaa gaaaggatca ggatcaggcg aagaactgct ctcaaaagat tatcatttgg
     6661 aaaatgaggt tgcacgttta aaaaagggaa gtggcagtgg tgaggaactt ctgtcgaaaa
     6721 attatcatct cgagaatgaa gtagcccgac ttaaaaaggg ttctggctcg ggtcagcggc
     6781 cgcagcgagc tcattcggga gaacatgcac atcaagctat acatggaggg caccgtggac
     6841 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     6901 atcagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacg
     6961 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     7021 aagcagtcct tccctcaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     7081 gtgctcaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     7141 atcagagggg tcaacttcac atccaacggc cctgtcatgc agaagaaaac actcggctgg
     7201 gaggccttca ccgagacgct gtaccccgcc gacggcggcc tggaaggtag aaacgacatg
     7261 gccctcaagc tcgtgggcgg gagccatctc atcgcaaaca tcaagaccac atacagatcc
     7321 aagaaacccg caaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     7381 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     7441 tactgcgacc tcccaagcaa actggggcac aaactcaatg ctagctaatg atcagttgta
     7501 caggtaccgg atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct
     7561 ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc
     7621 gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat atggcgtcgc
     7681 tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct gcagtattcc
     7741 cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga tatggcgtcg
     7801 ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc tgcagtattc
     7861 ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg atatggcgtc
     7921 gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg ctgcagtatt
     7981 cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac gatatggcgt
     8041 cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg gctgcagtat
     8101 tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga cgatatggcg
     8161 tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct ggctgcagta
     8221 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag acgatatggc
     8281 gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc tggctgcagt
     8341 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
     8401 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
     8461 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
     8521 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
     8581 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
     8641 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
     8701 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
     8761 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
     8821 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
     8881 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
     8941 gcagtattcc cgggttcatt agatctcgcg aagggcgaat tcacgaggcg cgcccggatc
     9001 gcgatcgcaa tcttaattaa ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     9061 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9121 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9181 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9241 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9301 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9361 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9421 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9481 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9541 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9601 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9661 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9721 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9781 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9841 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9901 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    10021 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
    10081 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
    10141 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
    10201 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
    10261 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
    10321 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
    10381 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
    10441 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
    10501 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
    10561 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
    10621 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    10681 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
    10741 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10801 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
    10861 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    10921 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    10981 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    11041 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    11101 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    11161 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    11221 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    11281 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    11341 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    11401 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    11461 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    11521 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    11581 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    11641 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    11701 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    11761 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    11821 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    11881 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    11941 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    12001 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    12061 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    12121 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    12181 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    12241 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    12301 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    12361 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    12421 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    12481 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    12541 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    12601 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    12661 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    12721 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    12781 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    12841 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    12901 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    12961 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    13021 acatttcccc gaaaagtgcc acctgacgtc
//
