LOCUS       sequence-31803-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, 
            Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz 
            CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M
  TITLE     Y65C missense mutation in the WW domain of the Golabi-Ito-Hall 
            syndrome protein PQBP1 affects its binding activity and deregulates 
            pre-mRNA splicing.
  JOURNAL   J Biol Chem. 2010 Jun 18;285(25):19391-401. doi: 
            10.1074/jbc.M109.084525. Epub 2010 Apr 21.
  PUBMED    20410308
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.084525"; journalName: "J Biol Chem"; date: 
            "2010-06-18- 18"; volume: "285"; issue: "25"; pages: "19391-401"
COMMENT     Sequence Label: pcDNA4-His-Max-A-PQBP1
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    82..244
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             257..274
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             278..310
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(314..322,323..337)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tagcgcagag gcttggggca gccgagcggc agccaggccc
      121 cggcccgggc ctcggttcca gaagggagag gagcccgcca aggcgcgcaa gagagcgggc
      181 tgcctcgcag tccgagccgg agagggagcg cgagccgcgc cggccccgga cggcctccga
      241 aaccatgggg ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
      301 gcaaatgggt cgggatctgt acgacgatga cgataaggta cctaggatcc aaatgccgct
      361 gcccgttgcg ctgcagaccc gcttggccaa gagaggcatc ctcaaacatc tggagcctga
      421 accagaggaa gagatcattg ccgaggacta tgacgatgat cctgtggact acgaggccac
      481 caggttggag ggcctaccac caagctggta caaggtgttc gacccttcct gcgggctccc
      541 ttactactgg aatgcagaca cagaccttgt atcctggctc tccccacatg accccaactc
      601 cgtggttacc aaatcggcca agaagctcag aagcagtaat gcagatgctg aagaaaagtt
      661 ggaccggagc catgacaagt cggacagggg ccatgacaag tcggaccgca gccatgagaa
      721 actagacagg ggccacgaca agtcagaccg gggccacgac aagtctgaca gggatcgaga
      781 gcgtggctat gacaaggtag acagagagag agagcgagac agggaacggg atcgggaccg
      841 cgggtatgac aaggcagacc ggggaagagg gcaaagaacg gcgccaccat cgccngggag
      901 gagctggctc cttatcccaa gagcaagaag gcagtaagcc gaaggatg
//
