LOCUS       sequence-318059-        4087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35s promoter for N-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Vazquez-Vilar M, Quijano-Rubio A, Fernandez-Del-Carmen A, 
            Sarrion-Perdigones A, Ochoa-Fernandez R, Ziarsolo P, Blanca J, 
            Granell A, Orzaez D
  TITLE     GB3.0: a platform for plant bio-design that connects functional DNA 
            elements with associated biological data.
  JOURNAL   Nucleic Acids Res. 2017 Feb 28;45(4):2196-2209. doi: 
            10.1093/nar/gkw1326.
  PUBMED    28053117
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-02-28- 28"; volume: "45"; issue: 
            "4"; pages: "2196-2209"
COMMENT     Sequence Label: pUPD P35S (GB0552)
FEATURES             Location/Qualifiers
     source          1..4087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        620..964
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     908..928
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        complement(1138..1156)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1139..1156)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1174..1190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1174..1190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1187..1209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1198..1214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1222..1228,1229..1246,1247..1252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1267..1288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1405..1422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1576..2164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1656..1675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2335..3126,3127..3195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2958..2977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3196..3300)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3378..3833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3515..3536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3727..3746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3959..3981
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3973..3990
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3974..3990
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3997..4016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3997..4015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4046..4055
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4050..4059
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggagac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
       61 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
      121 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
      181 gagaggccta cgcggcaggt ctgatcaaga cgatctaccc gagtaataat ctccaggaga
      241 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      301 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      361 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
      421 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
      481 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
      541 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
      601 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      661 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
      721 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca
      781 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      841 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      901 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      961 ggactccggt atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat
     1021 ttactattct agtcgaccat cgagaccact catcgcccat cactagtgaa ttcgcggccg
     1081 cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct
     1141 atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1201 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1261 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1321 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1381 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1981 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2461 tgcaatgata ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     2581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     2641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     2701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     2761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     2821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     2881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     2941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3301 cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga
     3361 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact
     3601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     3841 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3901 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3961 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     4021 ttgggcccga cgtcgcatgc tcccggccgc catggcggcc gcgggaattc gatgggcgat
     4081 gagtggt
//
