LOCUS       sequence-31807-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Carey BW, Markoulaki S, Beard C, Hanna J, Jaenisch R
  TITLE     Single-gene transgenic mouse strains for reprogramming adult somatic
            cells.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):56-9.
  PUBMED    20010831
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):56-9."
COMMENT     Sequence Label: mCol.4F2A targeting construct
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(354..407)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgattctggt gggttagcga gttggaaagg ataaagtcta ggtccaggag gtcgttgaac
       61 tcctcggtct ccctccgggc taggagggcc gggttgttac tgctgcaagc tgcaccagct
      121 ccgccactct ccaggtctgt ggccaccgtc gccgccaggt cgtaggggcg gccgggaagt
      181 gggggaagtc gcttcatgtg agagagttcc tcacgccaac ggttagtcgg ggcacctgct
      241 ggacgcagtg tcttctccct tcccgccggg ccggacgcga acgtggagaa ggacgggagc
      301 agagcgtcgc tgacagccat gtcagactcg ccaggtggct gcctcatctc gagtgggccg
      361 ggattttcct ccacgtcccc gcatgttaga agacttcccc tgccctcgcc ggagccgcat
      421 gccatgtgcg acaggggcag tgtgccgtta atggccgtgc cgggcaccgg gccgctctgg
      481 tagtgctggg ccatgtgcag tctactgggc gcagcgggct ccggcacctc ggcgccgggg
      541 aggtacatgc tgatcatgtc ccggaggtcc ccggcctggc agggcgccct ggagtgggag
      601 gaagaggtaa ccacgggggg gctggagctg gcctcggact tgaccacaga gcccatggag
      661 cccagcgcca taccgggggt gccctgctgc gagtaggaca tgctgt
//
