LOCUS       sequence-318087-        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Exression of ATI QconCAT protein in E. coli expression strains 
            (BL21). Encodes a concatemer of tryptic peptides from wheat 
            amylase/trypsin inhibitors. Used as standard for LC-MS-based 
            quantification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Sielaff M, Curella V, Neerukonda M, Afzal M, El Hassouni K, Distler 
            U, Schuppan D, Longin CFH, Tenzer S
  TITLE     Hybrid QconCAT-Based Targeted Absolute and Data-Independent 
            Acquisition-Based Label-Free Quantification Enables In-Depth 
            Proteomic Characterization of Wheat Amylase/Trypsin Inhibitor 
            Extracts.
  JOURNAL   J Proteome Res. 2021 Jan 28. doi: 10.1021/acs.jproteome.0c00752.
  PUBMED    33507751
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Proteome 
            Res. 2021 Jan 28. doi: 10.1021/acs.jproteome.0c00752."
COMMENT     Sequence Label: pET26b(+)-ATI_QconCAT_1
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcgg gcaaatacgt tgttgatacc agcaaactga ccgcggcgag cattaccgcg
       61 gtgtgccgtc tgccgattgt tgttgatgcg agcggtgacg gcgcgtacgt gtgcaaagat
      121 tgctgccagc aactggcgga catcagcgag tggtgccgta gccacaacag cggtccgtgg
      181 agctggtgca acccggcgac cggctataag ctgcagtgcg ttggtagcca agtgccggag
      241 gcggttctgc gtgactgctg ccaacaactg gcggacatca acaacgaatg gtgccgtagc
      301 gtttaccagg agctgggtgt gcgtgaatat gttgcgcagc aaacctgcgg tgtgggcatt
      361 gttggcagcc cggtgagcac cgagccgggt aacaccccgc gtgattacgt tctgcagcaa
      421 acctgcggca ccttcacccc gggtagcaaa ctgccggaat ggatgaccag cgcgagcatc
      481 tttagcccga tgaagccgta cctggcgaaa ctgtattgct gccaggagct ggcggaaatt
      541 ccgcagcaat gccgtctgac cgcggcgagc atcaccgcgg tgtgcaagct gccgatcgtt
      601 attgacgcga gcggtggccg ttactttatt gcgctgccgg tgccgagcca accggttgat
      661 ccgcgtagcg gtaacgtggg cgagagcggt ctgattgacc tgccgggttg cccgcgtgaa
      721 tatgtggcgc agcaaacctg cggcatcagc attagcggta gcgcggttag caccgaaccg
      781 ggcaacaccc cgcgtgattg ctgccaacag ctggcggaca tcagcgaatg gccgcgtatg
      841 gcggttaccc tgtggagcat ttggtatgcg cgtagccaca atagcggccc gtggagctgg
      901 tgcgatccgg cgaccggtta caagctgtac tgctgccaag agctggcgga aatcagccag
      961 caatgccgta ttgaaacccc gggcagcccg tacctggcga aacagcaatg ctgcggcgag
     1021 ctggcgaaca tcccgcaaca gtgccgtatt gaaatgccgg gtccgccgta tctggcgaag
     1081 caggagtgct gcgaacaact ggcgaacatc ccgcagcaat gccgtaccag catttacacc
     1141 tgctatgagg gtgtgggcct gccggttgat ccgctgcagg gttgccacta ctatgtgacc
     1201 agccaaacct gcggttttgt gccgctgctg ccgatcgaag ttatgaaatt aactgcggcg
     1261 agcgtgccgg cggtttgcaa ggtgccggcg ctgccgggtt gccgtccggt tctgaaactg
     1321 cagtgcgtgg gtaaccaagt tccggaagcg attcagcgtg actgctgcca acaactggcg
     1381 catatcagcg aatggtgccg catcggtgac tacgcgggta tcaagctcga g
//
