LOCUS       sequence-318124-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-all CRISPRi vector for targeting B. burgdorferi ftsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10TC)_Psyn-sgRNAftsI
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1404..1438
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1460..1535
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2978..2992,2993..2998,2999..3015,3016..3022)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2998..3014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3030..3046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3065..3076
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(3083..3109,3110..3112,3113..5599,5600..5602,
                     5603..7189)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7359..8150
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8273..8861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8762..8781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatttatga ctttcaataa taaaatgagg cttaaatttg taattttcgt atattttttg
       61 gaaacttgtt tctaattgtt tagaattata ttcatttttt tcaaattctt tttgagtgtt
      121 gcatagaatt tcctttaatt tttcttgttt ttctttcata caagatttag taaaatgtat
      181 atttttattt tttagtaatt taatttctat tatttttatg attttaagta ttttgattat
      241 attatcttta tcaatattta aattgaataa aattgtaaga aaaattgaaa gaattttttt
      301 acataaaaag ttacatttat tgaagtaatt tcttacttga tacttttcta tttcattaat
      361 ctttctttct tcttttatat tatttttatt atttaaacac tcctttaaat ctacactccc
      421 atttttagta aatctatctt taaggcccct tgaaactcta ttttgaaatc ttaaatcttt
      481 tttttcttga aagtatttat agattttttg gtaacattct ttttttgaaa aattaagttg
      541 atagtatatt tcggtcccca aatttacccc taagtgtttg tgataattag ttgtgacttt
      601 aaattctttt tctaatttgt aaaggtaatt ttgcagtgtt tttagtttaa cgggtgtttg
      661 actatttctt ttaaggtttt cattaaagta atagagtatg tttttttgtg tgtatttctt
      721 atatgtttta tttatgtacg ctagtgttga gataaggact actaatttat actgtaattt
      781 attgtagcaa gttgtggttt tactggtttt gtttatatta gataagtttt ccatttatag
      841 ctccttgaat accggatttt ttatctataa agtatatata tcacaatttt gatttaaaag
      901 taaattcttt ttttaaaaat acaaaaattt tttataaatt atatagacca tgatattgta
      961 ttaatactaa ttgattttaa tttaaggttg aactaaaata aacatagttt tgtagaaaat
     1021 aatttttcat tattttgcta cttgatattg gatcgtaaaa atattagggc tttacaaagt
     1081 tcttttaaaa gagaatttag taaagcccta agtcttttaa taaaaaattt tttgtaaaaa
     1141 agttggcaaa aatagttttt gctatatatt tatatataag aaaaaattca atactcaagg
     1201 agataaaaaa tggaaaactt aaacaataat ccacaaaaaa ttaatcaaat aaataagcaa
     1261 actaatttta ttggttttga aaattttata gaacttgata atcaagttaa tcaaactaaa
     1321 gaaaaaagta aattaagtaa gataggtaag aaattacccg gaataagcag tcaagtctag
     1381 tgcggccgga attctaaaga tctttgacag ctagctcagt cctaggtata atactagtga
     1441 gtgttattaa gcattctttg ttttagagct agaaatagca agttaaaata aggctagtcc
     1501 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggcgcgcc aaataatgcc
     1561 cgtgtccctg ttgaataggg acacggtttt ttaacaattt ttttattcct aggccttagt
     1621 ggccggtacc cggggatcct tattactggc cgctttctag cctggatact tgtctggcta
     1681 gttgcattag agagtcagct agagccctag ggctagctgt ttgtgtattc ggagccagtg
     1741 tagtttttct ttttactaac gatacgggta ataattgatt gccttttaca gcctgacctt
     1801 gagacaattg caaaagtctg tccacagagg tttgacctaa taatctaaaa tcttgtttga
     1861 tagtagtaag tggaggtatg tagcaactag aatcttctgt atcatcataa cccacaactg
     1921 aaatatcagc cccaactcta agtccacttt cagtaatagc tcgcatagct cctaaagcca
     1981 tttgatcatt agcaactaac atagcagtag ggacaatgcc ttcatttaac atttgcattg
     2041 tttgctgaaa tcctgacata gcagaccaat ctccttctct ttctgctatt ggttgtattt
     2101 gatttcttgt taaatacttg tgccatcctg ctaatctcaa tctggcactt actgaactta
     2161 agggacctgc aagtagtgca atttgttgat ggcctaatgc aacaaggtgt tcaactccta
     2221 accttgttcc atcttcgtga gaaaaaatta tagagtttat tggggtttgg tccgacacat
     2281 ccaaaaacaa tgctggaaca ttagtacatg ctgcttcaac tgcaatcgca tcttggtcat
     2341 caagaggata attaattata agtccactaa cacgttgagc taataaatta tgtactgctg
     2401 ccttacaagc ctctacacct gatctctcaa ccattgatac aacaacgctt gcaccaagtt
     2461 gatcagctct cgatttaatt gcggctacaa tttgactagg cgcatgtaat gcaagtgatg
     2521 atgtggcaac tccaattaaa agtgattgtt tccccgcaag ttgctgagca acgcgatttg
     2581 gtatataatt caactctgcc atagctgctt caaccttctc tcttgttttt gcagatacat
     2641 gacttgcttg gttcacaact ctcgaaaccg tttggtagct aaccccggca tattcagcaa
     2701 catcatacaa agtaacaggt ttcatggatc attcctccat gataaaattt aaatttctga
     2761 ctttggcaaa tccttttgcc tgttaaaaag aatagcacaa aaattaaaaa aaaagaaacg
     2821 caaatagcaa tagcattaaa aataattatt attatttatt tctttgatta aaatcaaaag
     2881 cctttaatgt attttcttga tctttcatgc gcttaacggt aaaatccaag gtattaagtt
     2941 caccttccac ttttctctct ctatcttctc tagaaaatca taaaaaattt atttgctttg
     3001 tgagcggata acaattataa cagattcaat tgtgagcgga taacaatttc acacagaatt
     3061 cattaaagag gagaaattac atatggataa gaaatactca ataggcttag ctatcggcac
     3121 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa
     3181 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt
     3241 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac
     3301 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt
     3361 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca
     3421 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc
     3481 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt
     3541 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga
     3601 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa
     3661 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc
     3721 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa
     3781 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa
     3841 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga
     3901 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc
     3961 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac
     4021 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac
     4081 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga
     4141 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta
     4201 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact
     4261 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca
     4321 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt
     4381 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg
     4441 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat
     4501 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga
     4561 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt
     4621 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg
     4681 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt
     4741 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga
     4801 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     4861 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     4921 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     4981 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     5041 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     5101 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     5161 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     5221 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     5281 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     5341 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     5401 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag
     5461 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     5521 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     5581 aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga
     5641 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga
     5701 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     5761 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     5821 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     5881 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     5941 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     6001 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt
     6061 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     6121 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac
     6181 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     6241 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     6301 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     6361 tattgtcaag aaaacagaag tccagacagg cggattctcc aaggagtcaa ttttaccaaa
     6421 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     6481 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     6541 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattctgg aaagaagttc
     6601 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga
     6661 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     6721 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     6781 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     6841 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     6901 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     6961 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     7021 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     7081 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac
     7141 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactaat aaaagcttga
     7201 tcagatctca gctttttttt gaagtgcctg gcagtaagtt gaaaaaattt gaaataaact
     7261 tggactatgt taataaaaaa atacaaatta acgaacaaaa agatttgaat tattatataa
     7321 tttagttaaa agcaatttta aatgagggag gtttccatat gagggaagcg gtgatcgccg
     7381 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     7441 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     7501 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     7561 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     7621 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     7681 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     7741 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     7801 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     7861 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     7921 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     7981 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     8041 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     8101 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgacgtcgcc
     8161 ggagcggtcg agttctggac cgaccggctc gggttctcat gaccaaaatc ccttaacgtg
     8221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8401 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcactagatc
     8941 cccggcaagt tcatccttag ggttttcata tctcttaaaa agtcaatttc tgcttgatta
     9001 atttcttgtg gattattgtt tttgcggttt ttcatttttt atctccttga gtattgaatt
     9061 ttttcttata tataaatata tagcaaaaac tatttttgcc aactttttta caaaaaattt
     9121 tttattaaaa cacttagggc tttgctaaat tctcttttaa aagaacttag taaagcccta
     9181 tggatttaag aactgattat acttacgtag taaaaatact acagatatta atagtataga
     9241 ccatatcaaa atcataatca atttatattt atctaaaatt gtagtccata caaaatacaa
     9301 aaaaattagg tttttctaaa ttctatttta aaagaactta gtaacaccta atctctaaat
     9361 tacttacaaa attctaaaat tagattttta gttcttcata tttctttaaa agttttttaa
     9421 gaaaatcttt ttgattttca aaaatttcat gcatcataaa actagtaaat ttggattttt
     9481 ttttgtaaaa atcataactt tcttgggttt taagttgaaa tcttaatggt tttattggat
     9541 tttgcctaga tttttttaac gccggacttt ctttatcttt tataaaatta taagaatttt
     9601 taataccatt ttctattaaa taatcttctt ttataagccc ctcttgcagt gcagttgcta
     9661 atcttaaata cttatacgct tgagtttttg ctacatcaaa gtcttttata aaatcaagaa
     9721 aagttttata accatcaaat ttataatatt gattatcttt tatttctttt aaaattttaa
     9781 tattttccac cctgtggaat atttcttttt tgaaattgtc atttaacttt tcctttagtg
     9841 tcttgtaata agaaatttta ctttcattaa tcattaaatt ttgttcaaca taagtatcta
     9901 tcttttttgt taaaacttta acaacacgtt ttttttccat aaaaattttc ctttattttt
     9961 gcaacatttt ccttaattga tataattgtt actattttag attttttaat atccacttaa
    10021 gaattaatcc ctcactagaa tattttttat tatagaattc atatacatgt ttttctaaat
    10081 ctataaattt atcaacaatt gctttattat agggtgtatc tatcttttct tttcttaata
    10141 atcgtctcat attctttaaa taacaacaaa cactgccggt tttaaaccta aattctatgt
    10201 aatatatttt tgaaaaagta tatacatttt caaccccgtt tatttcatac tttacaaaaa
    10261 cattatttat tggttttcta taaccatagt aaatgcctaa aaatttgtcc ccgtctttca
    10321 taggatatag ataaagtttt tgaatctctg atttatctaa taatttttca aaaaaaatta
    10381 tacaccgctt ttgtttgtta ctaactactc tatgcaatgc ctttaatatt tttgtgtgat
    10441 atatattttt atcacctttt ttttctttca gcaggaagat actttctttt ggaaatttaa
    10501 ttttttgttc atttattctt gcagccttaa gtccgatttt catatttaat ccttatatag
    10561 cctttctatt taattctttt aaactcaata aacttttttg gtttttcatt agttctaata
    10621 attcgcaata atatgtgcaa aacactttat tgtattctat tttcttttgg ttattcaaat
    10681 attcttttat aattggcttt agaagttcaa tatttacttc tttttttagt tgttcaataa
    10741 ggatattgaa aatgtttgtt tttaaatctt gataattttg ttgagaattt tctttttttc
    10801 tttcaattga cttctctaat ttacgcttta tattgtttaa atcgct
//
