LOCUS       sequence-318126-       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi rodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0526-sgRNArodA
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2232..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2312..2331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2943..3734)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(join(3904..5490,5491..5493,5494..7980,7981..7983,
                     7984..8010))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             8017..8028
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    8047..8063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8071..8077,8078..8094,8095..8100,
                     8101..8115))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    8079..8095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        complement(9558..9633)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gataatataa tcaaaatact taaaatcata aaaataatag aaattaaatt actaaaaaat
       61 aaaaatatac attttactaa atcttgtatg aaagaaaaac aagaaaaatt aaaggaaatt
      121 ctatgcaaca ctcaaaaaga atttgaaaaa aatgaatata attctaaaca attagaaaca
      181 agtttccaaa aaatatacga aaattacaaa tttaagcctc attttattat tgaaagtcat
      241 aaatatagcg atttaaacaa tataaagcgt aaattagaga agtcaattga aagaaaaaaa
      301 gaaaattctc aacaaaatta tcaagattta aaaacaaaca ttttcaatat ccttattgaa
      361 caactaaaaa aagaagtaaa tattgaactt ctaaagccaa ttataaaaga atatttgaat
      421 aaccaaaaga aaatagaata caataaagtg ttttgcacat attattgcga attattagaa
      481 ctaatgaaaa accaaaaaag tttattgagt ttaaaagaat taaatagaaa ggctatataa
      541 ggattaaata tgaaaatcgg acttaaggct gcaagaataa atgaacaaaa aattaaattt
      601 ccaaaagaaa gtatcttcct gctgaaagaa aaaaaaggtg ataaaaatat atatcacaca
      661 aaaatattaa aggcattgca tagagtagtt agtaacaaac aaaagcggtg tataattttt
      721 tttgaaaaat tattagataa atcagagatt caaaaacttt atctatatcc tatgaaagac
      781 ggggacaaat ttttaggcat ttactatggt tatagaaaac caataaataa tgtttttgta
      841 aagtatgaaa taaacggggt tgaaaatgta tatacttttt caaaaatata ttacatagaa
      901 tttaggttta aaaccggcag tgtttgttgt tatttaaaga atatgagacg attattaaga
      961 aaagaaaaga tagatacacc ctataataaa gcaattgttg ataaatttat agatttagaa
     1021 aaacatgtat atgaattcta taataaaaaa tattctagtg agggattaat tcttaagtgg
     1081 atattaaaaa atctaaaata gtaacaatta tatcaattaa ggaaaatgtt gcaaaaataa
     1141 aggaaaattt ttatggaaaa aaaacgtgtt gttaaagttt taacaaaaaa gatagatact
     1201 tatgttgaac aaaatttaat gattaatgaa agtaaaattt cttattacaa gacactaaag
     1261 gaaaagttaa atgacaattt caaaaaagaa atattccaca gggtggaaaa tattaaaatt
     1321 ttaaaagaaa taaaagataa tcaatattat aaatttgatg gttataaaac ttttcttgat
     1381 tttataaaag actttgatgt agcaaaaact caagcgtata agtatttaag attagcaact
     1441 gcactgcaag aggggcttat aaaagaagat tatttaatag aaaatggtat taaaaattct
     1501 tataatttta taaaagataa agaaagtccg gcgttaaaaa aatctaggca aaatccaata
     1561 aaaccattaa gatttcaact taaaacccaa gaaagttatg atttttacaa aaaaaaatcc
     1621 aaatttacta gttttatgat gcatgaaatt tttgaaaatc aaaaagattt tcttaaaaaa
     1681 cttttaaaga aatatgaaga actaaaaatc taattttaga attttgtaag taatttagag
     1741 attaggtgtt actaagttct tttaaaatag aatttagaaa aacctaattt ttttgtattt
     1801 tgtatggact acaattttag ataaatataa attgattatg attttgatat ggtctatact
     1861 attaatatct gtagtatttt tactacgtaa gtataatcag ttcttaaatc catagggctt
     1921 tactaagttc ttttaaaaga gaatttagca aagccctaag tgttttaata aaaaattttt
     1981 tgtaaaaaag ttggcaaaaa tagtttttgc tatatattta tatataagaa aaaattcaat
     2041 actcaaggag ataaaaaatg aaaaaccgca aaaacaataa tccacaagaa attaatcaag
     2101 cagaaattga ctttttaaga gatatgaaaa ccctaaggat gaacttgccg gggatctagt
     2161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2881 gggattttgg tcatgagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     2941 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     3001 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     3061 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     3121 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     3181 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     3241 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     3301 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     3361 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     3421 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     3481 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     3541 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     3601 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     3661 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     3721 accgcttccc tcatatggaa acctccctca tttaaaattg cttttaacta aattatataa
     3781 taattcaaat ctttttgttc gttaatttgt atttttttat taacatagtc caagtttatt
     3841 tcaaattttt tcaacttact gccaggcact tcaaaaaaaa gctgagatct gatcaagctt
     3901 ttattagtca cctcctagct gactcaaatc aatgcgtgtt tcataaagac cagtgatgga
     3961 ttgatggata agagtggcat ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat
     4021 tgttgtatca aaatatttaa aagcagcggg agctccaaga ttcgtcaacg taaataaatg
     4081 aataatattt tctgcttgtt cacgtattgg tttgtctcta tgtttgttat atgcactaag
     4141 aactttatct aaattggcat ctgctaaaat aacacgctta gaaaattcac tgatttgctc
     4201 aataatctca tctaaataat gcttatgctg ctccacaaac aattgttttt gttcgttatc
     4261 ttctggacta cccttcaact tttcataatg actagctaaa tataaaaaat tcacatattt
     4321 gcttggcaga gccagctcat ttcctttttg taattctccg gcactagcca gcatccgttt
     4381 acgaccgttt tctaactcaa aaagactata tttaggtagt ttaatgatta agtctttttt
     4441 aacttcctta tatcctttag cttctaaaaa gtcaatcgga tttttttcaa aggaacttct
     4501 ttccagaatt gtgatcccta gtaactcttt aacggatttt aacttcttcg atttcccttt
     4561 ttccacctta gcaaccacta ggactgaata agctaccgtt ggactatcaa aaccaccata
     4621 tttttttgga tcccagtctt ttttacgagc aataagcttg tccgaatttc tttttggtaa
     4681 aattgactcc ttggagaatc cgcctgtctg gacttctgtt ttcttgacaa tattgacttg
     4741 gggcatggac aatactttgc gcactgtggc aaaatctcgc cctttatccc agacaatttc
     4801 tccagtttcc ccattagttt cgattagagg gcgtttgcga atctctccat ttgcaagtgt
     4861 aatttctgtt ttgaagaagt tcatgatatt agagtaaaag aaatattttg cggttgcttt
     4921 gcctatttct tgctcagact tagcaatcat tttacgaaca tcataaactt tataatcacc
     4981 atagacaaac tccgattcaa gttttggata tttcttaatc aaagcagttc caacgacggc
     5041 atttagatac gcatcatggg catgatggta attgttaatc tcacgtactt tatagaattg
     5101 gaaatctttt cggaagtcag aaactaattt agattttaag gtaatcactt taacctctcg
     5161 aataagttta tcattttcat cgtatttagt attcatgcga ctatccaaaa tttgtgccac
     5221 atgcttagtg atttggcgag tttcaaccaa ttggcgtttg ataaaaccag ctttatcaag
     5281 ttcactcaaa cctccacgtt cagctttcgt taaattatca aacttacgtt gagtgattaa
     5341 cttggcgttt agaagttgtc tccaatagtt tttcatcttt ttgactactt cttcacttgg
     5401 aacgttatcc gatttaccac gatttttatc agaacgcgtt aagaccttat tgtctattga
     5461 atcgtcttta aggaaacttt gtggaacaat ggcatcgaca tcataatcac ttaaacgatt
     5521 aatatctaat tcttggtcca catacatgtc tcttccattt tggagataat agagatagag
     5581 cttttcattt tgcaattgag tattttcaac aggatgctct ttaagaatct gacttcctaa
     5641 ttctttgata ccttcttcga ttcgtttcat acgctctcgc gaatttttct ggcccttttg
     5701 agttgtctga ttttcacgtg ccatttcaat aacgatattt tctggcttat gccgccccat
     5761 tactttgacc aattcatcaa caacttttac agtctgtaaa ataccttttt taatagcagg
     5821 gctaccagct aaatttgcaa tatgttcatg taaactatcg ccttgtccag acacttgtgc
     5881 tttttgaatg tcttctttaa atgtcaaact atcatcatgg atcagctgca taaaattgcg
     5941 attggcaaaa ccatctgatt tcaaaaaatc taatattgtt ttgccagatt gcttatccct
     6001 aataccatta atcaattttc gagacaaacg tccccaacca gtataacggc gacgtttaag
     6061 ctgtttcatc accttatcat caaagaggtg agcatatgtt ttaagtcttt cctcaatcat
     6121 ctccctatct tcaaataagg tcaatgttaa aacaatatcc tctaagatat cttcattttc
     6181 ttcattatcc aaaaaatctt tatctttaat aatttttagc aaatcatggt aggtacctaa
     6241 tgaagcatta aatctatctt caactcctga aatttcaaca ctatcaaaac attctatttt
     6301 tttgaaataa tcttctttta attgcttaac ggttactttt cgatttgttt tgaagagtaa
     6361 atcaacaatg gctttcttct gttcacctga aagaaatgct ggttttcgca ttccttcagt
     6421 aacatatttg acctttgtca attcgttata aaccgtaaaa tactcataaa gcaaactatg
     6481 ttttggtagt actttttcat ttggaagatt tttatcaaag tttgtcatgc gttcaataaa
     6541 tgattgagct gaagcacctt tatcgacaac ttcttcaaaa ttccatgggg taattgtttc
     6601 ttcagacttc cgagtcatcc atgcaaaacg actattgcca cgcgccaatg gaccaacata
     6661 ataaggaatt cgaaaagtca agattttttc aatcttctca cgattgtctt ttaaaaatgg
     6721 ataaaagtct tcttgtcttc tcaaaatagc atgcagctca cccaagtgaa tttgatgggg
     6781 aatagagccg ttgtcaaagg tccgttgctt gcgcagcaaa tcttcacgat ttagtttcac
     6841 caataattcc tcagtaccat ccattttttc taaaattggt ttgataaatt tataaaattc
     6901 ttcttggcta gctcccccat caatataacc tgcatatccg ttttttgatt gatcaaaaaa
     6961 gatttcttta tacttttctg gaagttgttg tcgaactaaa gcttttaaaa gagtcaagtc
     7021 ttgatgatgt tcatcgtagc gtttaatcat tgaagctgat aggggagcct tagttatttc
     7081 agtatttact cttaggatat ctgaaagtaa aatagcatct gataaattct tagctgccaa
     7141 aaacaaatca gcatattgat ctccaatttg cgccaataaa ttatctaaat catcatcgta
     7201 agtatctttt gaaagctgta atttagcatc ttctgccaaa tcaaaatttg atttaaaatt
     7261 aggggtcaaa cccaatgaca aagcaatgag attcccaaat aagccatttt tcttctcacc
     7321 ggggagctga gcaatgagat tttctaatcg tcttgattta ctcaatcgtg cagaaagaat
     7381 cgctttagca tctactccac ttgcgttaat agggttttct tcaaataatt gattgtaggt
     7441 ttgtaccaac tggataaata gtttgtccac atcactatta tcaggattta aatctccctc
     7501 aatcaaaaaa tgaccacgaa acttaatcat atgcgctaag gccaaataga ttaagcgcaa
     7561 atccgcttta tcagtagaat ctaccaattt ttttcgcaga tgatagatag ttggatattt
     7621 ctcatgataa gcaacttcat ctactatatt tccaaaaata ggatgacgtt catgcttctt
     7681 gtcttcttcc accaaaaaag actcttcaag tcgatgaaag aaactatcat ctactttcgc
     7741 catctcattt gaaaaaatct cctgtagata acaaatacga ttcttccgac gtgtatacct
     7801 tctacgagct gtccgtttga gacgagtcgc ttccgctgtc tctccactgt caaataaaag
     7861 agcccctata agattttttt tgatactgtg gcggtctgta tttcccagaa ccttgaactt
     7921 tttagacgga accttatatt catcagtgat caccgcccat ccgacgctat ttgtgccgat
     7981 agctaagcct attgagtatt tcttatccat atgtaatttc tcctctttaa tgaattctgt
     8041 gtgaaattgt tatccgctca caattgaatc tattataatt gttatccgct cacaaagcaa
     8101 ataaattttt tatgattttc tagagaagat agagagagaa aagtggaagg tgaacttaat
     8161 accttggatt ttaccgttaa gcgcatgaaa gatcaagaaa atacattaaa ggcttttgat
     8221 tttaatcaaa gaaataaata ataataatta tttttaatgc tattgctatt tgcgtttctt
     8281 tttttttaat ttttgtgcta ttctttttaa caggcaaaag gatttgccaa agtcagaaat
     8341 ttaaatttta tcatggagga atgatccatg aaacctgtta ctttgtatga tgttgctgaa
     8401 tatgccgggg ttagctacca aacggtttcg agagttgtga accaagcaag tcatgtatct
     8461 gcaaaaacaa gagagaaggt tgaagcagct atggcagagt tgaattatat accaaatcgc
     8521 gttgctcagc aacttgcggg gaaacaatca cttttaattg gagttgccac atcatcactt
     8581 gcattacatg cgcctagtca aattgtagcc gcaattaaat cgagagctga tcaacttggt
     8641 gcaagcgttg ttgtatcaat ggttgagaga tcaggtgtag aggcttgtaa ggcagcagta
     8701 cataatttat tagctcaacg tgttagtgga cttataatta attatcctct tgatgaccaa
     8761 gatgcgattg cagttgaagc agcatgtact aatgttccag cattgttttt ggatgtgtcg
     8821 gaccaaaccc caataaactc tataattttt tctcacgaag atggaacaag gttaggagtt
     8881 gaacaccttg ttgcattagg ccatcaacaa attgcactac ttgcaggtcc cttaagttca
     8941 gtaagtgcca gattgagatt agcaggatgg cacaagtatt taacaagaaa tcaaatacaa
     9001 ccaatagcag aaagagaagg agattggtct gctatgtcag gatttcagca aacaatgcaa
     9061 atgttaaatg aaggcattgt ccctactgct atgttagttg ctaatgatca aatggcttta
     9121 ggagctatgc gagctattac tgaaagtgga cttagagttg gggctgatat ttcagttgtg
     9181 ggttatgatg atacagaaga ttctagttgc tacatacctc cacttactac tatcaaacaa
     9241 gattttagat tattaggtca aacctctgtg gacagacttt tgcaattgtc tcaaggtcag
     9301 gctgtaaaag gcaatcaatt attacccgta tcgttagtaa aaagaaaaac tacactggct
     9361 ccgaatacac aaacagctag ccctagggct ctagctgact ctctaatgca actagccaga
     9421 caagtatcca ggctagaaag cggccagtaa taaggatccc cgggtaccgg ccactaaggc
     9481 ctaggaataa aaaaattgtt aaaaaaccgt gtccctattc aacagggaca cgggcattat
     9541 ttggcgcgcc aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
     9601 cttattttaa cttgctattt ctagctctaa aactggtata ttaggtagat taacatgatg
     9661 attctaatca taaaaaatca aaatatcaat attgattgca taaaaacaaa aaaaataata
     9721 gaattatagg ttagctagtt aaataggagt aaaatatatg aaaattccta aaaattatat
     9781 tccaggaaca aacccttaca agtctctacc taaaaaacct atcattgaaa acaaggagct
     9841 cgaattcgta atcatgtcat agctgtttcc ggccgcacta gacttgactg cttattccgg
     9901 gtaatttctt acctatctta cttaatttac ttttttcttt agtttgatta acttgattat
     9961 caagttctat aaaattttca aaaccaataa aattagtttg cttatttatt tgattaattt
    10021 tttgtggatt attgtttaag ttttccattt tttatctcct tgagtattga attttttctt
    10081 atatataaat atatagcaaa aactattttt gccaactttt ttacaaaaaa ttttttatta
    10141 aaagacttag ggctttacta aattctcttt taaaagaact ttgtaaagcc ctaatatttt
    10201 tacgatccaa tatcaagtag caaaataatg aaaaattatt ttctacaaaa ctatgtttat
    10261 tttagttcaa ccttaaatta aaatcaatta gtattaatac aatatcatgg tctatataat
    10321 ttataaaaaa tttttgtatt tttaaaaaaa gaatttactt ttaaatcaaa attgtgatat
    10381 atatacttta tagataaaaa atccggtatt caaggagcta taaatggaaa acttatctaa
    10441 tataaacaaa accagtaaaa ccacaacttg ctacaataaa ttacagtata aattagtagt
    10501 ccttatctca acactagcgt acataaataa aacatataag aaatacacac aaaaaaacat
    10561 actctattac tttaatgaaa accttaaaag aaatagtcaa acacccgtta aactaaaaac
    10621 actgcaaaat tacctttaca aattagaaaa agaatttaaa gtcacaacta attatcacaa
    10681 acacttaggg gtaaatttgg ggaccgaaat atactatcaa cttaattttt caaaaaaaga
    10741 atgttaccaa aaaatctata aatactttca agaaaaaaaa gatttaagat ttcaaaatag
    10801 agtttcaagg ggccttaaag atagatttac taaaaatggg agtgtagatt taaaggagtg
    10861 tttaaataat aaaaataata taaaagaaga aagaaagatt aatgaaatag aaaagtatca
    10921 agtaagaaat tacttcaata aatgtaactt tttatgtaaa aaaattcttt caatttttct
    10981 tacaatttta ttcaatttaa atattgataa a
//
