LOCUS       sequence-31813-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, 
            Lieberman J
  TITLE     miR-34a contributes to megakaryocytic differentiation of K562 cells 
            independently of p53.
  JOURNAL   Blood. 2009 Sep 3. 114(10):2181-92.
  PUBMED    19584398
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2009
            Sep 3. 114(10):2181-92."
COMMENT     Sequence Label: pLL3.7_hsa-miR-30c
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        106..354
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     106..126
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     277..296
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     569..585
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     570..586
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(628..661)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
       61 cagtttggtt agtaccgggc ccgctctaga agatctgggc aggaagaggg cctatttccc
      121 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
      181 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      241 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      301 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagg atccccgttc
      361 tgtgtcagtg gtcaggggct gatcaaccct ggaccctgtg ggctataacc atgctgtagt
      421 gtgtgtaaac atcctacact ctcagctgtg agctcaaggt ggctgggaga gggttgttta
      481 ctccttctgc catggaaaac atcagctgaa gaaggaacag tctccactcc gcagatcgga
      541 cggaatgcga cctccctaat cgaattcctc gaggtcgacg gtatcgataa gctcgcttca
      601 cgagattcca gcaggtcgag ggacctaata acttcgtata gcatacatta tacgaagtta
      661 tattaagggt tccaagctta agcggccgcg tggataaccg tattaccgcc atgcattagt
      721 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      781 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      841 tcaataatga cgtatgttcc catagt
//
