LOCUS       sequence-318166-        2349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector backbone for defense systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG001 (empty vector)
FEATURES             Location/Qualifiers
     source          1..2349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..660
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(67..86)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(880..927)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1176..1721
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1622..1641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1838..1855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2247..2349
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
       61 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      121 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      181 cacattcttg cccgcctgat gaatgctcat ccagagttcc gtatggcgat gaaagacggt
      241 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      301 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      361 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      421 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
      481 gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc
      541 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat
      601 gtcggcagaa tgttaaatga attacaacag tattgcgatg agtggcaggg cggggcgtaa
      661 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
      721 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      781 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      841 cagtgtgacc gtgtgcttct caaatgcctg aggtttcagc aaaaaacccc tcaagacccg
      901 tttagaggcc ccaaggggtt atgctagtta ttgcgaattc tgcagaagct tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc
     1021 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actaacatga gaattacaac
     1081 ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc
     1141 tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta
     1201 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga
     1261 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
     1321 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
     1381 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
     1441 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     1501 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
     1561 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
     1621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     1681 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
     1741 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
     1801 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     1861 ggaatatatc ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc
     1921 acatgaagca cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc
     1981 gctagcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca gtagctgaac
     2041 aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac catcatacac
     2101 taaatcagta agttggcagc atcacccgac gcactttgcg ccgaataaat acctgtgacg
     2161 gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg
     2221 gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat
     2281 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     2341 gaagctaaa
//
