LOCUS       sequence-318167-        5895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Ec78 retron defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG008 (Ec78 retron)
FEATURES             Location/Qualifiers
     source          1..5895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      444..491
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(711..1370)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1285..1304
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1371..1473)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1865..1882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1999..2544)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2079..2098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttgtggcg attcaagaaa aagtggcggg tggtcacgtt gcggtatata taaggacaat
       61 ggtgctggcg cctacaatgc tgatgatctt ataaaacctg atgaagaaaa tcctgacgac
      121 tacctgctat ttctcactac tggagaggtt gtaccggcta tcggactcac ggggagagcg
      181 cttaaaaaag cgcaggaaac tatccgtgtt tttaacctga acggtgacat aaagttgttt
      241 ggcagtcgca gaactgcagt gcaagcaatc atgcctaatg tcgaatattt gtatactcta
      301 ctcgaagagt ttgacgaaga tgactggaat gaaatgctta gagatgagct cgaaaagata
      361 gaatctgatg aatacaaaac ggccctaaaa catgcatgga ctttcaacca agagttcgca
      421 taatcctaaa gaattcgcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      481 ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag
      541 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
      601 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
      661 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
      721 ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac atggaagcca
      781 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      841 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      901 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
      961 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1021 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1081 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1141 atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1201 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1261 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1321 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1381 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1441 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     1501 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     1561 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
     1621 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
     1681 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg
     1741 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     1801 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     1861 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     1921 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     1981 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     2041 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2101 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     2161 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     2221 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     2281 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     2341 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     2401 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     2461 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     2521 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     2581 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     2641 ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     2701 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
     2761 cgttgcgcaa gcttacgtgt cttgatttaa gttgacttca agactataaa gtctcaagta
     2821 acagtcggtt agcttccttc atgggttggt catgccgggt tgttaagtat ggctgtttgc
     2881 gataagcttt aaatactctt tagcgttgga cggttacgtc tagtcgggtg attagccaga
     2941 ctctaactta ttgaacgtat taagggttgc gaaagtgtcg caacccgaga tcgttcctct
     3001 ctcgggttgc gacactttcg cttcctcaag taaagagtga agcccggcgc aaatgcgccg
     3061 ggccattttc aggtactgtt atgtctgtta ttcgtggatt agctgcggtt ttacgtcaaa
     3121 gtgactccga tatcagcgcc tttcttgtaa ccgccccgag aaagtacaaa gtttacaaaa
     3181 tccctaagcg tacgacggga tttagagtca ttgcccagcc tgccaaaggg ctaaaagata
     3241 tccaacgagc ctttgttcag ctctatagcc tccctgttca tgatgcttca atggcctata
     3301 tgaaagggaa gggaattcgt gataatgctg cagcacatgc tggcaaccag tatctcctaa
     3361 aggcggatct ggaggatttt tttaactcaa ttacaccggc aattttttgg cgttgcattg
     3421 aaatgtcatc tgcgcaaaca cctcaatttg aacctcagga taagcttttt attgaaaaga
     3481 tccttttctg gcaaccgata aagcgtcgca aaaccaaatt gatattgagt gttggtgcgc
     3541 cttcttcacc agtcatatcc aatttctgta tgtatgagtt cgataatcga attcatgcgg
     3601 cttgcaagaa ggtggagata acatacacac gctatgcaga tgatctcacg ttctcgtcta
     3661 atatccctga tgtactgaaa gcagttcctt caacgcttga ggtcttactg aaggatttat
     3721 ttggaagcgc gctcagactt aatcacagca aaacggtttt ttcatcaaaa gcacataacc
     3781 ggcatgtgac tggtataaca ataaataatg aagagacact ttcactcggg cgcgatagaa
     3841 aaagatttat caaacatctg attaaccagt ataagtatgg actccttgat aatgaggata
     3901 aagcttatct gatcgggctg ttagcatttg ccagccatat cgagcctagt ttcatcacac
     3961 ggatgaacga aaaatactca ttagaactca tggaacgcct gagaggacag agatgaccaa
     4021 gcaatatgaa agaaaagcaa agggtggaaa tttactgtca gcattcgaac tttaccaacg
     4081 taatagtgat aaagcgcctg gtctgggtga aatgttagtg ggtgagtggt tcgaaatgtg
     4141 cagggattac attcaggatg gacatgttga tgagtcagga atatttcgtc cagataatgc
     4201 gttctatctt cgccgcctga cgttaaagga ttttcgccgt ttctctcttc tggaaattaa
     4261 actcgaagaa gatctgacag tcattattgg caacaatggt aaagggaaga caagtatctt
     4321 atatgcgatt gcaaaaacgc tgagttggtt cgtcgcgaac atcctgaagg aaggtggtag
     4381 tggacaaagg ttaagcgaaa tgactgacat aaaaaatgac gctgaagaca ggtattcaga
     4441 tgtcagtagc actttcttct ttggcaaagg acttaagagt gtgccgatca gattgtcacg
     4501 ctcagccctt ggtacagccg aaaggcggga cagcgaggtt aagcctgcca aggatttagc
     4561 tgatatatgg cgagtcatca atgaggtgaa tacgatcaac ttgccgacgt tcgctcttta
     4621 caacgttgag cgatcgcaac cgtttaaccg caacataaaa gataataccg gacgcagaga
     4681 agagcgcttt gatgcctata gtcaaacgct cggtggcgca ggacgtttcg atcatttcgt
     4741 tgagtggtac atttacctcc ataagcgtac tgtatcagat atctcaagtt ctattaaaga
     4801 acttgaacaa caggttaatg acttacagcg taccgttgat ggcggtatgg tttcggtaaa
     4861 atcacttctg gaacagatga agtttaagct tagtgaagct atagaaagaa atgatgctgc
     4921 ggtttcctcg agagtgttaa ctgagtctgt tcaaaaaagt attgttgaga aagcaatctg
     4981 ctcggttgtc cctagtatca gcaatatatg ggttgaaatg ataacgggtt ctgatttagt
     5041 caaagttaca aatgatgggc atgatgttac tattgaccaa ttatctgacg ggcagcgtgt
     5101 atttctgtcg ttggtggccg atcttgcgcg aagaatggtt atgctgaatc ccctgctgga
     5161 aaatccatta gagggacgtg gcattgtttt aattgatgaa atagaacttc accttcatcc
     5221 taagtggcag caggaagtta tcctgaacct gcgcagtgca ttccctaaca ttcaatttat
     5281 tattacaaca cacagtccca ttgttctttc tacaattgag aaacgctgta ttcgtgagtt
     5341 tgagcccaac gatgatggcg accaatcatt ccttgattct cccgatatgc aaacaaaggg
     5401 aagtgagaat gctcaaattc ttgagcaggt aatgaacgta cattctacac cgcctggtat
     5461 tgctgaatct cattggttag gtaattttga actattgctt ttagataatt ctggagaact
     5521 tgataaccac tctcaagtgc tttacgacca aatcaaggcg cactttggca tcgatagtat
     5581 tgagttgaag aaagcagata gccttattcg cattaataag atgaagaata aactgaacaa
     5641 gataagggcc gagaagggga aatagtaatg agagagttag cccggctgga gagaccggag
     5701 attcttgacc agtatatagc cggtcaaaat gactggatgg agattgatca gtctgcggta
     5761 tggccgaaat taactgaaat gcagggcgga ttttgtgcct attgcgagtg ccggttgaac
     5821 agatgtcata ttgagcattt caggccaagg ggaaagtttc ctgctctgac gtttatctgg
     5881 aataacctgt ttggt
//
