LOCUS       sequence-318171-        4315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ApeA defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG016 (ApeA)
FEATURES             Location/Qualifiers
     source          1..4315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2087..2134
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2354..3013)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2928..2947
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3014..3116)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3508..3525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3642..4187)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3722..3741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
       61 taatgagtga gctaacttac attaattgcg ttgcgcattt tagaaatatt gtgtaaaact
      121 tcttactctt tactggtcat ccctcagtcg tggaaaaaac acactgttcc atataggttt
      181 tatttgtgat ataatgaaca agttcttatt taagaaacct ataaacatta agcgacggaa
      241 atatatcatg aaaatagtca gcaataccgt ttgggatgga cttaaactgc ctgattatag
      301 ggctcgtttt tttatagaag tttggaagga gattttgtac gtcaacactc cttcatttta
      361 tcaatctaaa atgattaata cgatgtcagg tgccgaggag ttagtcgaag ccattgatga
      421 ttacatacaa gatgataaga gtaaaaaaag cttattatca atgatagaag attacaaagg
      481 taatttaaaa aaagactcta tagcaaaaga cacttttaaa aacttgcatg caacgctgtt
      541 aaaaaaaatt gagactgttc ctgacccaat atctagtaat tatattttag aattaaaaac
      601 aattgttaaa ttagtattat ccaaagaaag tgactattat cacgaactta aaaagcagct
      661 aaaatcatct attttgtcta acgctgattt gaataaaaaa gcccgtttaa tggactccat
      721 ttatcaatta actaaaagct ttattggcta tctcctgtgg aaggggtatt caccaactta
      781 tttatataat agaatggagt atcttacgag aattaaaaat tatggcagta gagacttttc
      841 cgctcaattt aatagttgcc ttgataaatt aactattagg attcatgatt atacagttta
      901 ttttcttatt acccctttgt ctaaatatct gattgaattg aataatatcc ttgatgttag
      961 ctttatcaat cgagaaggta ttattaatga aaaaaactac aataaaattt cacaaggggt
     1021 tgaatcttcg gtattagcca aaattgttgt taatacaaca gactacgttt ccgcggcgtg
     1081 gcaggcaaat gaaaaactgg ataaagtcat agattattta gaaatagaga agccagaata
     1141 taatattaga tattctcctg tatgtcttac agagttttca aatggtagat tcacacaccg
     1201 tcagactata aacataggca gattgaaaca attcattaca agtaaaaatt acagcattct
     1261 tgaaaatata cctaatgagt ccaaggtact cttacgagag tctataaaac tagacagata
     1321 tgatgtactg acaagatctt taaggtattt aagagttgca aaagaatcaa cttcacttga
     1381 gcaaaaattg ctgggcgtat ggatagctct tgaatgtatt ttcgagagca catcaggtaa
     1441 tatcatttct ggaataacta accatatccc tacgttctat agcactcaaa gtctagaaat
     1501 tagaattaga tattctaaag atttattaga agcccgattg aagcctattt cagatagcct
     1561 tttagagatt acagccaatc agaaatctaa atttcgagac ctttctttaa aagaatactt
     1621 tgacatagtg aaaatcgaaa aaaacaggaa taaaattttc gatgagttag tttccaaggg
     1681 ggatgagttt gccgtttttc gactaataaa aatatttgaa tcattcggaa cgtcaaagaa
     1741 aataaatgat agatttaatg atactaaaaa ggatgttgag tctcagcttt atagaattta
     1801 caaggtaaga aataaaataa cccatagagc atactacgga aatattaggc cccaattagt
     1861 ggatcatctt tatagctatt tactaagtgc atatagcaca ctaatttata gtttaagata
     1921 taatgcaata aataaatttg aaccacaaga tatgtttaat gcatatatta tctcgtgcga
     1981 gagtttaata ttcaatgttg aagaagaaaa aaaacttgaa aatataacta tggatgaaat
     2041 aattttatca tagtgaatgt tttctaggtg tcgtattccg caataactag cataacccct
     2101 tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat ttgagaagca
     2161 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     2221 tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga atttctgcca
     2281 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
     2341 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca atactgttgt aattcattta
     2401 acattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
     2461 tcagcacctt gtcgccttgc gtataatatt tgcccatagt gaaaacgggg gcgaagaagt
     2521 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     2581 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2641 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     2701 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2761 atatcaccag ctcaccgtct ttcatcgcca tacggaactc tggatgagca ttcatcaggc
     2821 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2881 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2941 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     3001 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     3061 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     3121 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     3181 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     3241 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     3301 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     3361 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     3421 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     3481 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     3541 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     3601 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     3661 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     3721 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     3781 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     3841 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     3901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     3961 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     4021 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     4081 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     4141 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     4201 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     4261 aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcc
//
