LOCUS       sequence-318172-        6749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the tmn defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG026 (tmn)
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4421..4468
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4688..5347)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5262..5281
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5348..5450)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5842..5859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5976..6521)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6056..6075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgctggcat gtttttacgg agtgacgctt tcattaacct gtacacgaac ttctattccg
       61 gcatcatgac aggcctgcag ccactgcgcc acttccagcg gatcgccctc ccggcgtacc
      121 actctgcctt ctttattcca taactgcaga caggtgctgc cgtcgagacg caccacaaaa
      181 tccccacggc aggcctgata ggggtttgag ggccaaccgt acgaaaacgt acggtaagag
      241 gaaaattatc gtcttaaaaa tcgatttatg ctatcacagt cgtctcttca ggtaagtacg
      301 gttgcctttg cctgctttct tctcgtctgg ttaagttaag aaattcagag atccatgctt
      361 gagataaaag cggaataaaa ccagtaaaat gtaactaaaa caacaacgga attgtatcaa
      421 tgataatgtc cacaccgtgg ctgacaccga tcgttgccga tagtgatcat gctgaggcaa
      481 atgcagtgag ctatgaagca ctgactccga cagaactcga ctcagataaa gcaggctgtt
      541 atatcagcgc gcttaattat gcttatgaac atccggatat ccggaatatt gctgttaccg
      601 ggccgtatgg ggcagggaaa agctcagtat taaaaacatg gtgcaaagct cacaatggga
      661 cactgcgggt gttaaccgtt tctcttgctg attttgatat gcagagacat gtggatgaaa
      721 gtaatgggga cagcagtagt gacgaaggga cgaaaaatac tggtagtgtt gaaaaatcta
      781 ttgaatacag tattctgcaa caaatactct acaaaaataa aaagcatgag cttccctgtt
      841 cccgcattga ccgtatatca gatgtgactg cgggacaaat attgcggtct gcgtcttttc
      901 tgacaggaac cattttactg agtggagctg ctttattttt ccttgcgccg gattacgtta
      961 caacaaagct atctttgccg ggagcattcg cccgttacct tcttgaatgc ccgtttgggg
     1021 tgcgtgtgtc cggtgcagtg gcatctgtga tgggatcgtt atgcctgctt ttgaaccagt
     1081 tacatcgtat cggtatattt gacaggaaag taagtcttga taaagtggac cttctgaaag
     1141 gcgctgttac aacccgggca tcatcacctt ctttacttaa tgtctatatt gatgaaattg
     1201 tctatttttt tgattcgact aaatatgatg tagtgatatt cgaagatctt gaccgtttta
     1261 acaatggccg gattttcgtg aaattgcggg aaatcaatca aattattaat aactgccttt
     1321 ctgacagaaa acctgtaaaa tttatttatg ctgtcagaga tggtattttc aactcagcag
     1381 agtcaagaac gaaattcttt gattttgtta tgcctgttat tccagtgatg gataaccaga
     1441 atgcttatga gcattttgtt aaaaaattca aagaagaaga gataaataat aacttaagcg
     1501 aatgtatttc tcgtattgcg acatttattc ccaatatgcg tgtaatgcat aatattacaa
     1561 atgagtttcg actctatcag aatttagtca atagtcggga aaatctggcc aaactacttg
     1621 ccatgatagc atataaaaat ctctgtgcgg aagattatca tggtatagat agtaaaaaag
     1681 gtgttcttta tcattttatt caaagctact tagaccatga aattcagaat gaattattac
     1741 attctgcaaa taacgaactt gaggatatgg cacagtcact tgtagcgata acaaatgaaa
     1801 aactcgcaaa ccgggaaaat ctgcgcgaag aactgctcat gccttacctt agtaaaaatt
     1861 atagcggcgc gcttgttttt tatacagaag gaaggcaaat aagtcttgat gatttgatac
     1921 aagatgaaga tgaatttctc atgcttttag ataaggaaaa tattcaggtc gttaccccct
     1981 ataacagaca aaattttctc atgataaatc agcgggatac agaaaaactg aagcagcagt
     2041 atgaaaaacg atgccattta attgaaacta aatctgttga taatataacc agagtgaaaa
     2101 ataatatttc cagtctggag tcattgagga ccgaaattct ttccggaact gtagctgata
     2161 tagcagaaaa gatgacaaat gaaggctttg ttgcctggat aaagaagaaa gaggatacag
     2221 gtgtcctgac gattcagtcg gaacatgaac agattgattt tatatttttt ctgttatcaa
     2281 gtggttattt atcaacagat tacatgtcct atcgctcaat cttcattccc ggagggctga
     2341 gtgagacaga taatttattt cttaaggatg ttatgtctgg taaaggtccg gaaaaaacat
     2401 tctcattcca tcttgataac gttaataata ttgttgaacg actcaaaaag ctgggggttc
     2461 tgcagcgtga caatgctcaa catcctgctg ttatcagatg gctgattgat aatgaccctg
     2521 ataccctgaa aaacaatata atggcattac tgagtcagac gggtagccag cgtgtggtta
     2581 gtttgctgat gttgatgcag aacgatttca caacgtatgt tcgcctgcgt tacctggaga
     2641 tttttatgtc agatgaacat atactgaaca gattgctggc acatttatgt gcgtcagaag
     2701 aacgcacacc cgagcaaaag ttttttgttc aggaaatagc ggcacacctg ttatgcctga
     2761 ctgaaaaatc aaatatctgg caatcggttg agattaataa acgtatcggt gagcttatag
     2821 attcctcccc aattcttatt actgctgtgc caaaaggata tggtgatgcg ttttttgaag
     2881 tgttgaaaga taatacactt tcagtttcat atattccagg tgatgtggga gacgagaagt
     2941 gttctgttat caggaaaatt gcgggtgcag gattattcaa atattccgtc agtaatctta
     3001 aaaatgttta tctttgcctg acgcaagaca agaatgaaga aagaatgtca ttctctcttt
     3061 atccgtttca ttgtctcgag tccctggcta tttctgaatt aacagaaatt ctgtggacta
     3121 acatagaaga ttttatttta tcggtattta ttgaatcgga agagattgat cgtattcctg
     3181 aattgctgaa ttcttctgaa gtctcaatga ctgttgttga acagattata gccaaaatgg
     3241 atttttgtat aaataatctg gatgatatta ttaatcgttc agagtgtgcg gacaataatg
     3301 cttcagggag aaatatctat agcatgctgt tgcagcatga caggattttt ccatcctttg
     3361 ataatattat tcatttattg catgatacat caattaatac ttccggtgaa cttgttcagt
     3421 gggtaaatga gaaacacttt gaatttgaac catctgatat agtcataaat gatacaggaa
     3481 tatttaataa ttttatttct gaattaattt gctcgccagt catttcagaa gaagctttac
     3541 tgaaagtact gagtaattta aacgttgtta ttatcgatgt gcctgaaaac attccattgc
     3601 gaaatgctga actgttatgt tcagagaaaa aactggcacc gacagttaat gtctttacgg
     3661 tgttgtttaa tgctctcagt gaaaatgttg atgatattaa caggatgaat actctgcttg
     3721 gtaaccttat tgcccagcgt cctgagatta ttacccagga gccagaagat attttttata
     3781 tcgagggtga ctttgatgaa gaactggcaa gcgaactttt tcgtcacaag ctaatcggta
     3841 tgaatataaa agttgccgct ttacgctggt tgcgtgataa caaaccggga attcttgata
     3901 agagctacct gctgtcatta gatattctgg cagaactgag tccctggatg ggtgacgatg
     3961 atctgcgcct gacactgctt aaacgttgtc tggttgccgg ggatgctggc aaagacgcgc
     4021 tttgcgtggt gctgaacagt tttgctgatg agagctatca tggactgtta ccacatgaca
     4081 ggttcaggaa aatccctcac tccgtggatt tgtgggaagt ggccgaatta atcagcaatc
     4141 ttggatttat tcagccgcca aaaatggggt cagggcgtga tgaacacaaa attgttatta
     4201 ctcccgtacg ctatgtccgt gatgttgagt tttatgactg agcatcattg atacggtgtt
     4261 ttaattgcct taaatacaaa aataaaaaca gattaatgct taatgtgcat taatctgttt
     4321 tagttatcaa tggctgttaa ttattgttaa ttttacatta atctttcttt ttcttcagga
     4381 agatccgaaa actcctggtc acggatcttc ctcgcaataa ctagcataac cccttggggc
     4441 ctctaaacgg gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt
     4501 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     4561 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     4621 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
     4681 aaaaaaatta cgccccgccc tgccactcat cgcaatactg ttgtaattca tttaacattc
     4741 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     4801 ccttgtcgcc ttgcgtataa tatttgccca tagtgaaaac gggggcgaag aagttgtcca
     4861 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     4921 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     4981 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     5041 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     5101 ccagctcacc gtctttcatc gccatacgga actctggatg agcattcatc aggcgggcaa
     5161 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     5221 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     5281 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     5341 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     5401 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     5461 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     5521 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     5581 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     5641 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     5701 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     5761 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     5821 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     5881 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     5941 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     6001 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     6061 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     6121 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     6181 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     6241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     6301 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     6361 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     6421 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     6481 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     6541 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
     6601 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga
     6661 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
     6721 ctaacttaca ttaattgcgt tgcgcaacc
//
