LOCUS       sequence-318173-        3942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DRT type 5 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG013 (DRT type 5)
FEATURES             Location/Qualifiers
     source          1..3942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1704..1751
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1971..2630)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2545..2564
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2631..2733)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3125..3142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3259..3804)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3339..3358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
       61 gctaacttac attaattgcg ttgcgcaaag tgaacggatg tatattgagt gcaatgtgat
      121 taactatctg ttgttacaat atttagatag gtgataaaat atgacatcta ccattgattt
      181 ttatgaatct gatttctcag ccacattata cccattaaaa accaatcaaa tattactcaa
      241 gcatcactca caagagatgt cagaatatat ttatcagaag gtcattaatc ctgcatatcc
      301 aacagatagt tttctgtctc agcaaaaagt cttttcgact aaacctaaag gtcatttgag
      361 acgaactgta aaattagatc cagtagctga gtattttatt tatgatgtta tctatcgaaa
      421 caggaagata tttaggccag aagtaagcga gtcgagaaaa agctttggat atatttttag
      481 gaacggtagc aggataccta tccacgtttc ctataatgaa tataaacaaa gcttaaaaaa
      541 atattctgag ctatattctc acagtataca ttttgacata gcatcttatt ttaatagttt
      601 atatcaccat gatataatcc actggtttag ctcaaaagaa ggagttagcc ctgcggatgt
      661 tgaagctctc ggacagtttt ttcgcgaaat taactcagga cgaagtatcg attttatgcc
      721 ccaaggaatt tatccggcaa aaatgatcgg taatgagttt ctaaaattcg ttgatttaca
      781 tggtcgccta aaatctgctc aaatagtaag atttatggat gactttacta tttttgacaa
      841 tgacattgaa acactaaata atgatttcat cagaatacag cagttattag ggcaagtatc
      901 cttaaatata aatccgtcaa aaaccacatt tgacaatgtg atgggagatg tgaatgaaac
      961 cttaactcag atcaagtcat cacttaaaga aatcattacg gaatatgaac atatacctac
     1021 agcctcaggg gtagaggtag tcgagactaa tattgaaatc ataaagcacc ttgatgatga
     1081 acaagttaac aaattaatag acttgctaaa agatgaaaaa atagaagagt ctgatgccga
     1141 tttaattctt ggttttttga gaactcataa tgatagttta ctttctcaga tgccaatgct
     1201 attaggcaga ttcccaaatt taataaaaca tatttatacg atctgttcag gtattaccga
     1261 taaatcagga ttagtaaaaa tattgctcag ctatttaaat actaataata actttttaga
     1321 atatcaattg ttttggattg gagcaatagt tgaagactat ctattaggtg taggtgagta
     1381 tggctccgtt ttacacaagt tatatgagtt atctggtgat tttaaaattg ccagagcaaa
     1441 agtattagag ataccggaac agggttttgg tttcaaagaa ataaggaatg aataccttag
     1501 aaccggacaa tcagattggt tatcatggtc ttcggctatc ggtacgagaa atcttaaatc
     1561 agcagagaga aactatattc ttgattattt ctcaaaaggc tcaccaataa attatcttgt
     1621 tgcatcttgc gtcaagaaac tttaatttaa aagccacctt cttgaaaggt ggctttaaaa
     1681 aataccttta gttcccgcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     1741 ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag
     1801 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     1861 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     1921 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     1981 ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac atggaagcca
     2041 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     2101 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2161 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     2221 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2281 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2341 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     2401 atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     2461 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     2521 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     2581 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     2641 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     2701 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     2761 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     2821 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
     2881 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
     2941 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg
     3001 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     3061 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     3121 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     3181 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     3241 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     3301 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3361 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     3421 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     3481 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     3541 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     3601 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     3661 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     3721 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     3781 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     3841 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     3901 ttgtaattct catgttagtc atgccccgcg cccaccggaa gg
//
