LOCUS       sequence-318174-        6785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DRT type 1 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG009 (DRT type 1)
FEATURES             Location/Qualifiers
     source          1..6785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4463..4510
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4730..5389)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5304..5323
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5390..5492)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5884..5901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6018..6563)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6098..6117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagtcctta aacacgacaa aacctgtgat acttaccatg gattcctcta tgaaggaaag
       61 gtagtatagc cattttgggt gatacataca gtgaatgtca ttgctgtagt tgaagtgagt
      121 aagagcgctt aagattaagt tgagagaaaa tgaaactact tgataaaaag tattacaacc
      181 tcgagcccaa atatgagtac cttaaggact catttatttt aggactggca tggaaaaaaa
      241 cagatagttt tgtaagaact cacaattggt atgcagatat tttagagctg gacaagtgtg
      301 cgtttgatat tagtgatgaa gtcactaatt ggtcaaacga gatctcaaag aacgctcttt
      361 ccaaaagtga tattgaattg ataccggctc caaaaggagc aagctggttc attaatcaag
      421 gtaaatggac taccaataaa gataatagaa agataaggcc tttggctaac atatctatta
      481 gggatcagtc ttttgctaca gcagtaacaa tgtgccttgc tgatgctata gaaacaagac
      541 agaaagactg ttcgttgagc aatcttggct atgctgagca tgtaaagaac aaggttgtta
      601 gttacggaaa taggcttgtc tgcgattggg acaatgaaag ggcaagattt cgttggggag
      661 gaagtgaata ttataggaag ttctcttccg attatcgaag ctttctacaa agacctatct
      721 atataggcag ggaaacagta aataaagtta gcggaattga tgatgtatat atcatcagtt
      781 tagatctgaa aaattttttc ggttctataa aaataaacct tctgttagaa aaaatcaaaa
      841 aaatatccgc tgatcattat gcagctaaat tcataaatga taatgaattt tggactttgg
      901 cgaatcggat tttaagttgg gattggcctg aagaatcttt atctttactt gagagtttgg
      961 atataaaaga aaaaaatgtt ggtcttcccc agggattagc ttctgctggt gctctggcga
     1021 atgcatatct cattgagttt gatgaatctt taatttctaa gcttcgtact aagatagaag
     1081 acagccaaat aatactgcat gattattgtc gatatgtcga tgatattaga ttagtgattt
     1141 caggagaagc actagaaagt aataagatta aggaatctat tcatgcatta gttcagggca
     1201 ttcttgatga gacattggct caaaatccgt cagataatga accatattta aaaattaacg
     1261 atagcaagac ttatattctt gagctttcag acattgacaa cggaagtggg cttacaaatc
     1321 gaatcaatga aattcagcat gaagtaggag cttcgagtat cccagagcgt aacggactcg
     1381 ataataatat cccggcactt caacaattat tactgaccga acaggataat ttttccgagg
     1441 atgttgatag tttatttccc gggtttaaaa atgataagtc gataaaggta gaatctgtac
     1501 gtagattttc tgcccatagg ctggaaaaaa gtttggctaa aaaaagcaag ctaatttcac
     1561 ctgaggagag gaaacaattt gataatgaaa cctcactgat tgcaaaaaaa ttattaaaag
     1621 cttggctaaa agatccatca attatggtta tcttccgcaa agcgatagct atcaatccta
     1681 atctagatgc ttatagcacc attcttgaaa ttattttttc aagaatacaa cgcaatcgtg
     1741 ataaacgaga taaatatata atgctgtatc ttctttctga tatatttcgt agcgtcattg
     1801 atgtctatcg aaacctagaa tcagaatacg tcgacgatta tcaaaaattg atgggtgaag
     1861 ttacattgtt tgcccaaaaa atactttcct gcaaatcttt tattccaaat tacgcatatc
     1921 agcaagcatt attttatctc gcagtgatca ataaaccatt tatagctagt aataaagctt
     1981 cttttgatct tgcaaggctt caatgcgtct taattaaaca gcatttagaa ccgttgaata
     2041 gtagtgatgg atacctattt gaggtatctg ctcaaatcag taaagactac cgagcaaatg
     2101 ccgcttttct actttctcat acaaatagta acaaagtagt agacttaatt atcgaaaaat
     2161 ttgctttccg aggaggtgaa ttctggaatg caatttggaa agaaattgtt aggatgcaag
     2221 ataaagatag gattaacgaa tttagatggg ccatatcaaa atatgagtca aagccaaata
     2281 gttcggagca ctatctttca tcagtgatca gtttcaagga aaacccattt agatatgaac
     2341 atgcgcttct caagctaggt gtagcattag ttgaactctt tgatgataca gagaaaaacg
     2401 tatggcaacc tgatggtaag cagtattctc cacatgaaat aaaagtaaaa ttagaaggta
     2461 actcaacctc atggggtgaa ttatggcgtc caaattttag tatttcatgc tcgatagata
     2521 agaaaggtga acctggtaaa gacccacgct atataagccc tgagtggttg gcaaattatc
     2581 cacagactca aaatgatgaa caaaaaatct attgggtttg cagtgtgcta agaagtgctg
     2641 ctttaggcaa tgtagattat actcaaagaa atgatttaaa acttgataaa gctaagtatg
     2701 atggtatcca ttctcagttt tacaagcgac gtatgggaat gttacataca ccagagtcaa
     2761 ttgttggttc atatggaact ataacagatt ggtttgcaag ttttcttcag catggattgc
     2821 aatggccagg tttttcttct tcgtatataa gccaagaaga tatattgtca attactaata
     2881 ttattgagtt taaaaactgt ttattggaac ggctaggcta cttaaataag cagatatgta
     2941 tttcatcgaa tgttccaacc ttaccgactg ttgtcaacag gcctgaatta gcatctaacc
     3001 attttagaat tgttacggtt cagcagttat ttcctaagga tactaatttc catccttctg
     3061 acgtgacttt ggctaatccc gatgtgcgct ggaagcacag agagcacctt gcggaaatct
     3121 gtaagctaac ggagcaaact ttaaatgcaa aacttaaaac tgagtctagg gaacatacaa
     3181 gcacagctga tctaatcgtt ttttctgagt tagcagttca cccagaagat gaagatatag
     3241 ttagagcact ggcatttaga accaaagcca tcattttttc cggctttgtc ttctgtgaac
     3301 aagatggccg aatagttaac aaagctcgtt ggattattcc agactcttca gagtctggga
     3361 cccaatggcg tgtccgtgat caggggaaac atcatatgac cagtgatgaa gtggctcttg
     3421 gcattcaagg atatagacca tcccaacata ttatttcaat tgagggtcac cctgagggac
     3481 catttaaatt aactggtgcg atttgctacg atgcaacaga tataaagctt gcggcagatc
     3541 tgagagattt gactgacatg tttgtcattg cagcatacaa taaagatgta gacacatttg
     3601 ataatatggc ttcagcacta caatggcata tgtatcagca tattgttatt acgaatacgg
     3661 gagaatatgg aggctcaact atgcaagccc cgtacaaaga gaaatatcat aaattgattt
     3721 ctcatgctca tgggactggt caaatagcaa ttagtactgc tgatatagat ttagcagcat
     3781 tcaggcggaa gctacaaata tataaaaaga ccaaaaccca gcctgctgga tacaatagaa
     3841 aacattaagg atttttatgg atactttagt taagttagct acaattattt ctccattaat
     3901 tagtgctgga gtagctattt gggcaatttt ggttgctaaa aaaaccatca gtgaaagcaa
     3961 agaaattgcc aagaaaacca tcgctgatac ggcctaccaa gcatatttgc aattagccat
     4021 ggagaaccca caattttcga aaggctacag cgcagattgt agacaggagc gagaccctat
     4081 gtatgatcaa tatgtttggt acgtggctag gatgatattc tgctttgaga aaatcatcga
     4141 ggttgaagta aacttaaaag atagttcttg ggcaaatacg ttggaaaaac atttgaagtt
     4201 tcattctgaa cattttaaga aaacgaatgt tgtcgaagag gctctctata ttccccctat
     4261 tttggatctc ataagatgtg cagctaacta ataacttatc ccaataggat tatattccac
     4321 acgataagcc cactggaaaa tgtaacatcc caagatagtt tttgggattg tttcccagtg
     4381 ggcggaaagt atcatgatag ttgtcacccc cggtggagct gcaaagattt ttatggggtg
     4441 ggtgttacat tgcgcgcaat aactagcata accccttggg gcctctaaac gggtcttgag
     4501 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
     4561 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
     4621 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
     4681 caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     4741 cctgccactc atcgcaatac tgttgtaatt catttaacat tctgccgaca tggaagccat
     4801 cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     4861 aatatttgcc catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     4921 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     4981 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     5041 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     5101 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     5161 tcgccatacg gaactctgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     5221 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     5281 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     5341 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     5401 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     5461 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     5521 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     5581 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
     5641 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
     5701 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
     5761 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
     5821 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
     5881 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     5941 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     6001 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     6061 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6121 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     6181 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     6241 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     6301 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     6361 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     6421 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     6481 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     6541 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     6601 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     6661 tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     6721 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     6781 gttgc
//
