LOCUS       sequence-318175-        8292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the hhe defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG028 (hhe)
FEATURES             Location/Qualifiers
     source          1..8292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6009..6056
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6276..6935)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6850..6869
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6936..7038)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7430..7447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7564..8109)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7644..7663)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg cacggattga atctgtttat
       61 gaaatttggc tgctatcaac taatgggcgt taagttgatt gtatgatctg attgataaag
      121 aaggggagac taaaaatctc ctcttctttg cagcagttta ctgcggtctt tttgtgatgc
      181 atcagcataa aacgttttac ttgtggaccc taagaaatgg agaacattat gtcgactgta
      241 gatacctcta cagcagagga actcaatcaa ggaggctcag attttattct gacttccctc
      301 gaggctatgc gtaagaagtt attggacctt acgtctcgaa atcgactttt gaatttccct
      361 atcactcaaa aagggtcttc actacgtatt gttgatgaat taccagaaca gctttatgaa
      421 accctttgct cggaaatccc gatggaattt gctcctgtgc ccgatccaac tagagcgcag
      481 ctgttagagc atggctatct caaagttggg ccagatggta aagatataca gttaagagct
      541 catcctagcg ctaaggattg ggcgcacgtc ttaggaatcc gtacagattt tgatttacca
      601 gatagccata aaacggttgt ttctgattca gatagagagt tgctggaaaa agcccatcag
      661 tttatcttgc aatatgccca aggccagaat ggaaaattaa cagggattcg ttctgaatac
      721 gttaatcaag gtatagcttt gtcagcgttg aaggaggcgt gctgcttagc aggctatgaa
      781 gggcttgagg attttgaacg acaggcaaag gctgggaatg agattagtat atcttcttcc
      841 aatccctctc atgacgataa tcggatacag gctctgcttt atccaaatga actggaagct
      901 tgtttgcgcg ccatctatgg taaggctcaa actgctttgg aggagagtgg cgccaacatc
      961 ttgtatttgg cgttagggtt ccttgagtgg tatgaaagcg attcctctga aaaggcacgt
     1021 tatgcaccgt tatttacaat tccggtgaga tgtgaacgag gaaaattaga tccgaaggat
     1081 ggtctttaca agtttcaact ttattacacg ggtgaagata ttttgcccaa tctctctttg
     1141 aaggaaaaac ttcaggctga ctttggcctc gctcttcctt tgttcaatga agaggaaact
     1201 ccagagtctt attttgcttc ggtgaagaag gttgtagagc agcacaaacc taaatggtct
     1261 gtgaaacgtt atggtgcact tagcttgctc aattttggca agatgatgat gtatcttgac
     1321 ctcgatcctg cccgctggcc ttgtgacaag cgcaatatat tgtctcatga agtaattcgt
     1381 cgctttttca ccagtcagag ctgtggtcaa gagaattccg gcttacctgg tggcttcggt
     1441 cagcatgagt actgcatcga tagttaccct gatattcatg acaaggttcc actaatcgat
     1501 gatgcggata gctcgcagca cagtgcgttg atcgatgcta tccgtggtca aaacttagtc
     1561 attgagggcc ctcctggtag tggcaaatca caaacgatca ccaacttgat tgcagcagct
     1621 ctgctcaacg gtaagaaagt cctgtttgtg gcagagaaga tggctgcact ggaggttgtc
     1681 aaacgtcgct tggatcgtgc ggggctaggt caattttgct tagagttgca cagtcataaa
     1741 actcataagc gcaaggtgct ggatgatatt aatgctcgct tggtgagtca ggcgaccatg
     1801 cctactatgg aagagattga tgctcagatt ttgcgttatg aagatcttaa gcagcagctc
     1861 aatgaatatg ccgcattgat caataaccaa tgggcgcaaa caggcaaaac gatccatcag
     1921 attttgagtg gtgcaacccg ttatcgtcac aaattagata ttgatgcaac agcacttcat
     1981 atcgaaaacc tttccgggaa gcagttggat aaagtgaccc aattacggct gcgtgaccaa
     2041 atagtagaat ttagccgcat ctacaaagag gttcgtgagc aggtgggggc taatgcagaa
     2101 atatatgagc acccttggag cggtgtgaat aacacacaaa ttcaattgtt tgacagcgct
     2161 cgtatagtcg atttgctaca aacttggcag acatcaatta tcgactttca acatagctat
     2221 caagaatatg tagataagtg ggcgttagaa ggcgaaagcc ttaatacgct tcaatatatt
     2281 gagcaattgg tagaagatca gtcgaatctt ccagtgttgt gtggttcaga gcatttccca
     2341 gcacttagtg agctagattc acccgatgcc attgcacggg tgcgtcacta tttagatagg
     2401 ttcgagttgc tacaaggtca ttatgtggcc ttgagccagg ttatcgagcc tcaaaagcta
     2461 cgacttttag aacaaggaca atcgtgtgac tttcctcgtg aagagctgga aaaatatggt
     2521 gcagcagagg atttcacttt acgtgatttg gtcaggtggc ttgaatccat ccaatcaatt
     2581 catgatgagt tatcatctat ttatgcgcaa ttaaacgatt tcaaaaatgc tttgccagat
     2641 ggtattgctt cgtatatcga tgattcgcaa gctggattgc tattctgctc tgagttgttg
     2701 tcgattctgg gtgctttacc gactgagctt attagagttc gagatcctct ttttgatgat
     2761 gatgatatcg atgcagtatt gcgcgactta atgtgtcaaa tcgaaacatt gcgtccttta
     2821 agagatggtc tatctacttt gtatcaattg gaccagttgc cttcccaaga gatgctcgcg
     2881 catgccgttg ctgttatcca gcaaggggga ttatttgcat ggtttaagag tgattggcgt
     2941 agtgccaagg cactgctcat ggcgcaatct cgaaagcctg acactaagtt tgctgagtta
     3001 aaacgctgct cagctgattt gctcaagtat tcggagctgt tacaacggtt tgaacaaagt
     3061 gactttggta atcaacttgg taatgcattc cgagggttgg acaccgactg tgaacaactc
     3121 atgttattgc gtgattggta caagaaggtc cgagcttgtt acgggatagg ttttggaaag
     3181 cgagttgcga taggctctgg attatttaac ctagatggtg agattatcaa aggtgtgcat
     3241 ttaatcgaga aatcgcagat tagctcaaga ttaatgactt tggttaaacg ggtcgagcac
     3301 gaggctaagt tattaccgcg tatttctagc ttgttggaag aacatgcatc ttggttaggt
     3361 gagcaaggtg tattgatgca atcttaccga caggtgcgga atactctcat tgccttgcag
     3421 ggatggttta tcaatccaga tatatcatta gagcagatga ctcattcctc cgagattttg
     3481 caaaacataa acgatcttca gatatccctt gaaaatgact cgttacagtt aggggcgttt
     3541 ttacaattaa ccccattggc ttgcggtgcg tataaaaata atcaactgac gttagacact
     3601 attaacgaca cgctgaattt tgccgagcaa ctggttgata agataaattg cgtatccttg
     3661 gctacccaga tcagacattt ggctagtggt agtgattacg atttactatg tcgtgatggt
     3721 ggagaaatag tttcgaaatg gaatgaacag attaaaaatg ctgagttata tgcgctagaa
     3781 acaaagttag agcggagtca gtggctcaag tcgactgatg gttctcttaa tacattaatc
     3841 gagcgcaacg aaagagcaat acagcaaccc cgttggttga acgggtgggt taactttatt
     3901 cgttgttacg agcagatgca tgaaaatgga ttgcagcgaa tctggagtgc tgtacttgcg
     3961 ggctcgctcc cgattgaaaa agttgaattg ggtttagcat tagcaattca tgaccagctg
     4021 gcgcgggagg ttattcacat ccaccctgaa ttgatgagag tttccggctc acagcgcaat
     4081 gctttgcaga agtcatttaa agagtacgac aaaaaactga ttgaattaca acgtcagcgg
     4141 attgcagcaa aaattgcttg ccgaaatata ccagaaggga attctggtgg taagaaaagt
     4201 gaatatacag aactagcttt gatcaaaaat gagttgggta aaaaaaccag acatattcca
     4261 attaggcaat tggttaaccg tgcatgtaat gcgctggttg caattaaacc ttgtttcatg
     4321 atggggccaa tgtcagcagc tcattaccta gaacctggac gaatggaatt tgatctggtg
     4381 gtgatggacg aagcgtctca ggtgaagcca gaggatgcat tgggtgtcat cgcgaggggc
     4441 aagcaactag tggtcgttgg tgacccgaaa cagctaccac caaccagttt ctttgatcga
     4501 agtgccgacg gagaagatga cgatgatgcc gcggctttaa gtgatactga cagcattttg
     4561 gatgctgctt tgccactgtt tcctatgaga cgtttgcgtt ggcactatcg ttcacgacat
     4621 gaaaagttga ttgcatactc taaccgccat ttttataaca gtgatttggt gatattccct
     4681 tccccaaatg ctgagtctcc agagtatggg attaaattta cctatgtgtc aaaaggtcgg
     4741 ttctccaatc aacacaatat tgaagaagcc caagcagttg ctgaggccgt acttcatcat
     4801 gcgcatcacc ggccgggtga gtcactcggg gtagtggcca tgagttccaa gcaacgcgat
     4861 caaattgagc gcgctatcga tgaattgcgc cgaaatcgcc ctgaatttaa cgatgcaatc
     4921 gatggcttac atgccatgga agagccactt tttgtgaaaa accttgagaa cgttcaaggg
     4981 gatgagcgtg atgtaatctt tatttccttt acctatggac cttctgagca tggtggaaag
     5041 gtttatcaac gctttggacc tatcaattcc gatgttggct ggcgtcgctt gaatgtgctt
     5101 ttcactcgat caaaaaaacg gatgcatgtg tttagttcaa tgcgttctga agatgtattg
     5161 acgagtgaaa ccagtaaact tggtgttatt tcgttgaaag gttttttaca gtttgccgaa
     5221 agtggcaaac tagattccct cacaacgcat accggcaggg ctccagatag tgactttgag
     5281 gttgctgtaa tggaagcact caatcacgct gggtttgagt gtgaacctca ggtaggggtt
     5341 gcaggattct ttattgatct agctgtgaaa gatccaggtt gtcctggccg ttatttaatg
     5401 ggcatagagt gtgatggtgc ggcttatcac tcagctaaat ctgctcgtga tcgtgaccgt
     5461 ttgcgtcaag aggttctgga gcgtttgggt tggagaatta gccgcatttg gtccactgat
     5521 tggttcagta atcctgatga ggttctatct ccgattatcc gtaaactcca tgagcttaaa
     5581 acattggctc cagacgttgt tgtaccttcc tatgaatatg tcgaaacgat tgagtcaagc
     5641 gctgaagtgg cgtctgactc aattgattct cttatgccca atttggggct taaggagcaa
     5701 cttaagtatt ttgccacaca tgtcattgag gttgagcttc ctaatgttga tgctgatcgt
     5761 cgtttgttgc ggcccgcaat gcttgaggct ttgctggaac atcagccttt atcacgttcc
     5821 gagtttgttg aacgaatacc tcattatctg cggcaagcaa cagatgtata cgaagcacaa
     5881 cgctttcttg accgagtctt ggcattaatt gatggcgcag aggctgaagc gaatgatgca
     5941 gcgtttgagt ctgaattggc ataattagtt aaaggtaata agaacagtga caactgtcgg
     6001 cgcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6061 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
     6121 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga
     6181 atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca
     6241 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     6301 caatactgtt gtaattcatt taacattctg ccgacatgga agccatcaca gacggcatga
     6361 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccata
     6421 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     6481 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     6541 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     6601 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     6661 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcatcgc catacggaac
     6721 tctggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     6781 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     6841 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     6901 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     6961 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     7021 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     7081 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     7141 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     7201 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     7261 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct
     7321 tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg
     7381 ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg
     7441 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     7501 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     7561 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     7621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     7681 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     7741 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     7801 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7861 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     7921 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     7981 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     8041 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     8101 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     8161 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
     8221 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     8281 gtcgagatcc cg
//
