LOCUS       sequence-318178-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi mreB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10TC)_Psyn-sgRNAmreB
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..85
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        107..182
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(1625..1639,1640..1645,1646..1662,1663..1669)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1645..1661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1677..1693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1712..1723
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1730..1756,1757..1759,1760..4246,4247..4249,
                     4250..5836)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNKKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6006..6797
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6920..7508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7409..7428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttacccggaa taagcagtca agtctagtgc ggccggaatt ctaaagatct ttgacagcta
       61 gctcagtcct aggtataata ctagtgatat ctattgcaac aacagagttt tagagctaga
      121 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      181 gctttttttg gcgcgccaaa taatgcccgt gtccctgttg aatagggaca cggtttttta
      241 acaatttttt tattcctagg ccttagtggc cggtacccgg ggatccttat tactggccgc
      301 tttctagcct ggatacttgt ctggctagtt gcattagaga gtcagctaga gccctagggc
      361 tagctgtttg tgtattcgga gccagtgtag tttttctttt tactaacgat acgggtaata
      421 attgattgcc ttttacagcc tgaccttgag acaattgcaa aagtctgtcc acagaggttt
      481 gacctaataa tctaaaatct tgtttgatag tagtaagtgg aggtatgtag caactagaat
      541 cttctgtatc atcataaccc acaactgaaa tatcagcccc aactctaagt ccactttcag
      601 taatagctcg catagctcct aaagccattt gatcattagc aactaacata gcagtaggga
      661 caatgccttc atttaacatt tgcattgttt gctgaaatcc tgacatagca gaccaatctc
      721 cttctctttc tgctattggt tgtatttgat ttcttgttaa atacttgtgc catcctgcta
      781 atctcaatct ggcacttact gaacttaagg gacctgcaag tagtgcaatt tgttgatggc
      841 ctaatgcaac aaggtgttca actcctaacc ttgttccatc ttcgtgagaa aaaattatag
      901 agtttattgg ggtttggtcc gacacatcca aaaacaatgc tggaacatta gtacatgctg
      961 cttcaactgc aatcgcatct tggtcatcaa gaggataatt aattataagt ccactaacac
     1021 gttgagctaa taaattatgt actgctgcct tacaagcctc tacacctgat ctctcaacca
     1081 ttgatacaac aacgcttgca ccaagttgat cagctctcga tttaattgcg gctacaattt
     1141 gactaggcgc atgtaatgca agtgatgatg tggcaactcc aattaaaagt gattgtttcc
     1201 ccgcaagttg ctgagcaacg cgatttggta tataattcaa ctctgccata gctgcttcaa
     1261 ccttctctct tgtttttgca gatacatgac ttgcttggtt cacaactctc gaaaccgttt
     1321 ggtagctaac cccggcatat tcagcaacat catacaaagt aacaggtttc atggatcatt
     1381 cctccatgat aaaatttaaa tttctgactt tggcaaatcc ttttgcctgt taaaaagaat
     1441 agcacaaaaa ttaaaaaaaa agaaacgcaa atagcaatag cattaaaaat aattattatt
     1501 atttatttct ttgattaaaa tcaaaagcct ttaatgtatt ttcttgatct ttcatgcgct
     1561 taacggtaaa atccaaggta ttaagttcac cttccacttt tctctctcta tcttctctag
     1621 aaaatcataa aaaatttatt tgctttgtga gcggataaca attataacag attcaattgt
     1681 gagcggataa caatttcaca cagaattcat taaagaggag aaattacata tggataagaa
     1741 atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
     1801 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
     1861 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
     1921 caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
     1981 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
     2041 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
     2101 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
     2161 ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
     2221 tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact
     2281 atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag
     2341 tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa
     2401 tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt
     2461 gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt
     2521 acagctttca aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga
     2581 tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga
     2641 tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg
     2701 ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc
     2761 agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga
     2821 tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga
     2881 tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac
     2941 ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag
     3001 aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt
     3061 gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg
     3121 gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa
     3181 aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa
     3241 taaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt
     3301 gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca
     3361 gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt
     3421 aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga
     3481 agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa
     3541 agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac
     3601 cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga
     3661 tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg
     3721 aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag attttttgaa
     3781 atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt
     3841 taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat
     3901 tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt
     3961 tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc
     4021 acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat
     4081 cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac
     4141 tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt
     4201 ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca
     4261 aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg
     4321 tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag
     4381 acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga
     4441 acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac
     4501 tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga
     4561 tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc
     4621 tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc
     4681 ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact
     4741 tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa
     4801 gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa
     4861 cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga
     4921 aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg
     4981 caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtcc agacaggcgg
     5041 attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa
     5101 agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct
     5161 agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact
     5221 agggatcaca attctggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc
     5281 taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt
     5341 tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa
     5401 tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa
     5461 gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca
     5521 ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga
     5581 tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga
     5641 acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt
     5701 taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga
     5761 tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca
     5821 gctaggaggt gactaataaa agcttgatca gatctcagct tttttttgaa gtgcctggca
     5881 gtaagttgaa aaaatttgaa ataaacttgg actatgttaa taaaaaaata caaattaacg
     5941 aacaaaaaga tttgaattat tatataattt agttaaaagc aattttaaat gagggaggtt
     6001 tccatatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6061 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     6121 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     6181 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6241 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     6301 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6361 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     6421 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     6481 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     6541 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     6601 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     6661 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     6721 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     6781 aggtagtcgg caaataatga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     6841 ttctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7561 ctcacatgtt ctttcctgca ctagatcccc ggcaagttca tccttagggt tttcatatct
     7621 cttaaaaagt caatttctgc ttgattaatt tcttgtggat tattgttttt gcggtttttc
     7681 attttttatc tccttgagta ttgaattttt tcttatatat aaatatatag caaaaactat
     7741 ttttgccaac ttttttacaa aaaatttttt attaaaacac ttagggcttt gctaaattct
     7801 cttttaaaag aacttagtaa agccctatgg atttaagaac tgattatact tacgtagtaa
     7861 aaatactaca gatattaata gtatagacca tatcaaaatc ataatcaatt tatatttatc
     7921 taaaattgta gtccatacaa aatacaaaaa aattaggttt ttctaaattc tattttaaaa
     7981 gaacttagta acacctaatc tctaaattac ttacaaaatt ctaaaattag atttttagtt
     8041 cttcatattt ctttaaaagt tttttaagaa aatctttttg attttcaaaa atttcatgca
     8101 tcataaaact agtaaatttg gatttttttt tgtaaaaatc ataactttct tgggttttaa
     8161 gttgaaatct taatggtttt attggatttt gcctagattt ttttaacgcc ggactttctt
     8221 tatcttttat aaaattataa gaatttttaa taccattttc tattaaataa tcttctttta
     8281 taagcccctc ttgcagtgca gttgctaatc ttaaatactt atacgcttga gtttttgcta
     8341 catcaaagtc ttttataaaa tcaagaaaag ttttataacc atcaaattta taatattgat
     8401 tatcttttat ttcttttaaa attttaatat tttccaccct gtggaatatt tcttttttga
     8461 aattgtcatt taacttttcc tttagtgtct tgtaataaga aattttactt tcattaatca
     8521 ttaaattttg ttcaacataa gtatctatct tttttgttaa aactttaaca acacgttttt
     8581 tttccataaa aattttcctt tatttttgca acattttcct taattgatat aattgttact
     8641 attttagatt ttttaatatc cacttaagaa ttaatccctc actagaatat tttttattat
     8701 agaattcata tacatgtttt tctaaatcta taaatttatc aacaattgct ttattatagg
     8761 gtgtatctat cttttctttt cttaataatc gtctcatatt ctttaaataa caacaaacac
     8821 tgccggtttt aaacctaaat tctatgtaat atatttttga aaaagtatat acattttcaa
     8881 ccccgtttat ttcatacttt acaaaaacat tatttattgg ttttctataa ccatagtaaa
     8941 tgcctaaaaa tttgtccccg tctttcatag gatatagata aagtttttga atctctgatt
     9001 tatctaataa tttttcaaaa aaaattatac accgcttttg tttgttacta actactctat
     9061 gcaatgcctt taatattttt gtgtgatata tatttttatc accttttttt tctttcagca
     9121 ggaagatact ttcttttgga aatttaattt tttgttcatt tattcttgca gccttaagtc
     9181 cgattttcat atttaatcct tatatagcct ttctatttaa ttcttttaaa ctcaataaac
     9241 ttttttggtt tttcattagt tctaataatt cgcaataata tgtgcaaaac actttattgt
     9301 attctatttt cttttggtta ttcaaatatt cttttataat tggctttaga agttcaatat
     9361 ttacttcttt ttttagttgt tcaataagga tattgaaaat gtttgttttt aaatcttgat
     9421 aattttgttg agaattttct ttttttcttt caattgactt ctctaattta cgctttatat
     9481 tgtttaaatc gctatattta tgactttcaa taataaaatg aggcttaaat ttgtaatttt
     9541 cgtatatttt ttggaaactt gtttctaatt gtttagaatt atattcattt ttttcaaatt
     9601 ctttttgagt gttgcataga atttccttta atttttcttg tttttctttc atacaagatt
     9661 tagtaaaatg tatattttta ttttttagta atttaatttc tattattttt atgattttaa
     9721 gtattttgat tatattatct ttatcaatat ttaaattgaa taaaattgta agaaaaattg
     9781 aaagaatttt tttacataaa aagttacatt tattgaagta atttcttact tgatactttt
     9841 ctatttcatt aatctttctt tcttctttta tattattttt attatttaaa cactccttta
     9901 aatctacact cccattttta gtaaatctat ctttaaggcc ccttgaaact ctattttgaa
     9961 atcttaaatc ttttttttct tgaaagtatt tatagatttt ttggtaacat tctttttttg
    10021 aaaaattaag ttgatagtat atttcggtcc ccaaatttac ccctaagtgt ttgtgataat
    10081 tagttgtgac tttaaattct ttttctaatt tgtaaaggta attttgcagt gtttttagtt
    10141 taacgggtgt ttgactattt cttttaaggt tttcattaaa gtaatagagt atgttttttt
    10201 gtgtgtattt cttatatgtt ttatttatgt acgctagtgt tgagataagg actactaatt
    10261 tatactgtaa tttattgtag caagttgtgg ttttactggt tttgtttata ttagataagt
    10321 tttccattta tagctccttg aataccggat tttttatcta taaagtatat atatcacaat
    10381 tttgatttaa aagtaaattc tttttttaaa aatacaaaaa ttttttataa attatataga
    10441 ccatgatatt gtattaatac taattgattt taatttaagg ttgaactaaa ataaacatag
    10501 ttttgtagaa aataattttt cattattttg ctacttgata ttggatcgta aaaatattag
    10561 ggctttacaa agttctttta aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa
    10621 ttttttgtaa aaaagttggc aaaaatagtt tttgctatat atttatatat aagaaaaaat
    10681 tcaatactca aggagataaa aaatggaaaa cttaaacaat aatccacaaa aaattaatca
    10741 aataaataag caaactaatt ttattggttt tgaaaatttt atagaacttg ataatcaagt
    10801 taatcaaact aaagaaaaaa gtaaattaag taagataggt aagaaa
//
