LOCUS       sequence-31818-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, 
            Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz 
            CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M
  TITLE     Y65C missense mutation in the WW domain of the Golabi-Ito-Hall 
            syndrome protein PQBP1 affects its binding activity and deregulates 
            pre-mRNA splicing.
  JOURNAL   J Biol Chem. 2010 Jun 18;285(25):19391-401. doi: 
            10.1074/jbc.M109.084525. Epub 2010 Apr 21.
  PUBMED    20410308
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.084525"; journalName: "J Biol Chem"; date: 
            "2010-06-18- 18"; volume: "285"; issue: "25"; pages: "19391-401"
COMMENT     Sequence Label: pcDNA4-His-Max-A-PQBP1-Y65C
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    75..237
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             250..267
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             271..303
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(307..315,316..330)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt
       61 ttaaacttaa gcttagcgca gaggcttggg gcagccgagc ggcagccagg ccccggcccg
      121 ggcctcggtt ccagaaggga gaggagcccg ccaaggcgcg caagagagcg ggctgcctcg
      181 cagtccgagc cggagaggga gcgcgagccg cgccggcccc ggacggcctc cgaaaccatg
      241 gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      301 ggtcgggatc tgtacgacga tgacgataag gtacctagga tccaaatgcc gctgcccgtt
      361 gcgctgcaga cccgcttggc caagagaggc atcctcaaac atctggagcc tgaaccagag
      421 gaagagatca ttgccgagga ctatgacgat gatcctgtgg actacgaggc caccaggttg
      481 gagggcctac caccaagctg gtacaaggtg ttcgaccctt cctgcgggct cccttactgc
      541 tggaatgcag acacagacct tgtatcctgg ctctccccac atgaccccaa ctccgtggtt
      601 accaaatcgg ccaagaagct cagaagcagt aatgcagatg ctgaagaaaa gttggaccgg
      661 agccatgaca agtcggacag gggccatgac aagtcggacc gcagccatga gaaactagac
      721 aggggccacg acaagtcaga ccggggccac gacaagtctg acagggatcg agagcgtggc
      781 tatgacaagg tagacagaga gagagagcga gacagggaac gggatcggga accgcgggta
      841 tgacaaggca gaccgggaag agggcaaaaa cggcgccacc atcgccggga ggagctggct
      901 ccntatccaa gagcaaaang cagtaagccg aaaggatgaa gagtaaccct gttggaccnt
      961 agntta
//
