LOCUS       sequence-318193-       11042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_P0826L-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..11042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        325..400
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(1843..1857,1858..1863,1864..1880,1881..1887)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1863..1879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1895..1911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1930..1941
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1948..1974,1975..1977,1978..4464,4465..4467,
                     4468..6054)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6224..7015
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7138..7726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7627..7646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt gcggccggaa
       61 acagctatga catgattacg aattcgagct cggcaataga agaatctata gaaagcataa
      121 aaagccttat agaaaacaaa gaatacatac aaaagttaca ttttataaaa aaattaataa
      181 ataaggttta caaaaaatta aattactttt tttaaaaact aaactttgaa agccttgtta
      241 taatataaaa tataataatc aaatattatt caaagttaac agcaatgaag tttataataa
      301 attgaaaatt taaatttctg acttgtttta gagctagaaa tagcaagtta aaataaggct
      361 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttggc gcgccaaata
      421 atgcccgtgt ccctgttgaa tagggacacg gttttttaac aattttttta ttcctaggcc
      481 ttagtggccg gtacccgggg atccttatta ctggccgctt tctagcctgg atacttgtct
      541 ggctagttgc attagagagt cagctagagc cctagggcta gctgtttgtg tattcggagc
      601 cagtgtagtt tttcttttta ctaacgatac gggtaataat tgattgcctt ttacagcctg
      661 accttgagac aattgcaaaa gtctgtccac agaggtttga cctaataatc taaaatcttg
      721 tttgatagta gtaagtggag gtatgtagca actagaatct tctgtatcat cataacccac
      781 aactgaaata tcagccccaa ctctaagtcc actttcagta atagctcgca tagctcctaa
      841 agccatttga tcattagcaa ctaacatagc agtagggaca atgccttcat ttaacatttg
      901 cattgtttgc tgaaatcctg acatagcaga ccaatctcct tctctttctg ctattggttg
      961 tatttgattt cttgttaaat acttgtgcca tcctgctaat ctcaatctgg cacttactga
     1021 acttaaggga cctgcaagta gtgcaatttg ttgatggcct aatgcaacaa ggtgttcaac
     1081 tcctaacctt gttccatctt cgtgagaaaa aattatagag tttattgggg tttggtccga
     1141 cacatccaaa aacaatgctg gaacattagt acatgctgct tcaactgcaa tcgcatcttg
     1201 gtcatcaaga ggataattaa ttataagtcc actaacacgt tgagctaata aattatgtac
     1261 tgctgcctta caagcctcta cacctgatct ctcaaccatt gatacaacaa cgcttgcacc
     1321 aagttgatca gctctcgatt taattgcggc tacaatttga ctaggcgcat gtaatgcaag
     1381 tgatgatgtg gcaactccaa ttaaaagtga ttgtttcccc gcaagttgct gagcaacgcg
     1441 atttggtata taattcaact ctgccatagc tgcttcaacc ttctctcttg tttttgcaga
     1501 tacatgactt gcttggttca caactctcga aaccgtttgg tagctaaccc cggcatattc
     1561 agcaacatca tacaaagtaa caggtttcat ggatcattcc tccatgataa aatttaaatt
     1621 tctgactttg gcaaatcctt ttgcctgtta aaaagaatag cacaaaaatt aaaaaaaaag
     1681 aaacgcaaat agcaatagca ttaaaaataa ttattattat ttatttcttt gattaaaatc
     1741 aaaagccttt aatgtatttt cttgatcttt catgcgctta acggtaaaat ccaaggtatt
     1801 aagttcacct tccacttttc tctctctatc ttctctagaa aatcataaaa aatttatttg
     1861 ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca atttcacaca
     1921 gaattcatta aagaggagaa attacatatg gataagaaat actcaatagg cttagctatc
     1981 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
     2041 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
     2101 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
     2161 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
     2221 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
     2281 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
     2341 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
     2401 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
     2461 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
     2521 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
     2581 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
     2641 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
     2701 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
     2761 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
     2821 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
     2881 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     2941 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     3001 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
     3061 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
     3121 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc
     3181 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
     3241 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat
     3301 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
     3361 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
     3421 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
     3481 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
     3541 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
     3601 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
     3661 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
     3721 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
     3781 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
     3841 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
     3901 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
     3961 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
     4021 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
     4081 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
     4141 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
     4201 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
     4261 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
     4321 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
     4381 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
     4441 cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca
     4501 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
     4561 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
     4621 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
     4681 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
     4741 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
     4801 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
     4861 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
     4921 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
     4981 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
     5041 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
     5101 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
     5161 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
     5221 gtcaatattg tcaagaaaac agaagtccag acaggcggat tctccaagga gtcaatttta
     5281 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
     5341 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
     5401 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tctggaaaga
     5461 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
     5521 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
     5581 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
     5641 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
     5701 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
     5761 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
     5821 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
     5881 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
     5941 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
     6001 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga ctaataaaag
     6061 cttgatcaga tctcagcttt tttttgaagt gcctggcagt aagttgaaaa aatttgaaat
     6121 aaacttggac tatgttaata aaaaaataca aattaacgaa caaaaagatt tgaattatta
     6181 tataatttag ttaaaagcaa ttttaaatga gggaggtttc catatgaggg aagcggtgat
     6241 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     6301 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     6361 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     6421 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     6481 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     6541 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     6601 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     6661 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     6721 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     6781 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     6841 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     6901 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     6961 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgacg
     7021 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcatgacca aaatccctta
     7081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcact
     7801 agatccccgg caagttcatc cttagggttt tcatatctct taaaaagtca atttctgctt
     7861 gattaatttc ttgtggatta ttgtttttgc ggtttttcat tttttatctc cttgagtatt
     7921 gaattttttc ttatatataa atatatagca aaaactattt ttgccaactt ttttacaaaa
     7981 aattttttat taaaacactt agggctttgc taaattctct tttaaaagaa cttagtaaag
     8041 ccctatggat ttaagaactg attatactta cgtagtaaaa atactacaga tattaatagt
     8101 atagaccata tcaaaatcat aatcaattta tatttatcta aaattgtagt ccatacaaaa
     8161 tacaaaaaaa ttaggttttt ctaaattcta ttttaaaaga acttagtaac acctaatctc
     8221 taaattactt acaaaattct aaaattagat ttttagttct tcatatttct ttaaaagttt
     8281 tttaagaaaa tctttttgat tttcaaaaat ttcatgcatc ataaaactag taaatttgga
     8341 tttttttttg taaaaatcat aactttcttg ggttttaagt tgaaatctta atggttttat
     8401 tggattttgc ctagattttt ttaacgccgg actttcttta tcttttataa aattataaga
     8461 atttttaata ccattttcta ttaaataatc ttcttttata agcccctctt gcagtgcagt
     8521 tgctaatctt aaatacttat acgcttgagt ttttgctaca tcaaagtctt ttataaaatc
     8581 aagaaaagtt ttataaccat caaatttata atattgatta tcttttattt cttttaaaat
     8641 tttaatattt tccaccctgt ggaatatttc ttttttgaaa ttgtcattta acttttcctt
     8701 tagtgtcttg taataagaaa ttttactttc attaatcatt aaattttgtt caacataagt
     8761 atctatcttt tttgttaaaa ctttaacaac acgttttttt tccataaaaa ttttccttta
     8821 tttttgcaac attttcctta attgatataa ttgttactat tttagatttt ttaatatcca
     8881 cttaagaatt aatccctcac tagaatattt tttattatag aattcatata catgtttttc
     8941 taaatctata aatttatcaa caattgcttt attatagggt gtatctatct tttcttttct
     9001 taataatcgt ctcatattct ttaaataaca acaaacactg ccggttttaa acctaaattc
     9061 tatgtaatat atttttgaaa aagtatatac attttcaacc ccgtttattt catactttac
     9121 aaaaacatta tttattggtt ttctataacc atagtaaatg cctaaaaatt tgtccccgtc
     9181 tttcatagga tatagataaa gtttttgaat ctctgattta tctaataatt tttcaaaaaa
     9241 aattatacac cgcttttgtt tgttactaac tactctatgc aatgccttta atatttttgt
     9301 gtgatatata tttttatcac cttttttttc tttcagcagg aagatacttt cttttggaaa
     9361 tttaattttt tgttcattta ttcttgcagc cttaagtccg attttcatat ttaatcctta
     9421 tatagccttt ctatttaatt cttttaaact caataaactt ttttggtttt tcattagttc
     9481 taataattcg caataatatg tgcaaaacac tttattgtat tctattttct tttggttatt
     9541 caaatattct tttataattg gctttagaag ttcaatattt acttcttttt ttagttgttc
     9601 aataaggata ttgaaaatgt ttgtttttaa atcttgataa ttttgttgag aattttcttt
     9661 ttttctttca attgacttct ctaatttacg ctttatattg tttaaatcgc tatatttatg
     9721 actttcaata ataaaatgag gcttaaattt gtaattttcg tatatttttt ggaaacttgt
     9781 ttctaattgt ttagaattat attcattttt ttcaaattct ttttgagtgt tgcatagaat
     9841 ttcctttaat ttttcttgtt tttctttcat acaagattta gtaaaatgta tatttttatt
     9901 ttttagtaat ttaatttcta ttatttttat gattttaagt attttgatta tattatcttt
     9961 atcaatattt aaattgaata aaattgtaag aaaaattgaa agaatttttt tacataaaaa
    10021 gttacattta ttgaagtaat ttcttacttg atacttttct atttcattaa tctttctttc
    10081 ttcttttata ttatttttat tatttaaaca ctcctttaaa tctacactcc catttttagt
    10141 aaatctatct ttaaggcccc ttgaaactct attttgaaat cttaaatctt ttttttcttg
    10201 aaagtattta tagatttttt ggtaacattc tttttttgaa aaattaagtt gatagtatat
    10261 ttcggtcccc aaatttaccc ctaagtgttt gtgataatta gttgtgactt taaattcttt
    10321 ttctaatttg taaaggtaat tttgcagtgt ttttagttta acgggtgttt gactatttct
    10381 tttaaggttt tcattaaagt aatagagtat gtttttttgt gtgtatttct tatatgtttt
    10441 atttatgtac gctagtgttg agataaggac tactaattta tactgtaatt tattgtagca
    10501 agttgtggtt ttactggttt tgtttatatt agataagttt tccatttata gctccttgaa
    10561 taccggattt tttatctata aagtatatat atcacaattt tgatttaaaa gtaaattctt
    10621 tttttaaaaa tacaaaaatt ttttataaat tatatagacc atgatattgt attaatacta
    10681 attgatttta atttaaggtt gaactaaaat aaacatagtt ttgtagaaaa taatttttca
    10741 ttattttgct acttgatatt ggatcgtaaa aatattaggg ctttacaaag ttcttttaaa
    10801 agagaattta gtaaagccct aagtctttta ataaaaaatt ttttgtaaaa aagttggcaa
    10861 aaatagtttt tgctatatat ttatatataa gaaaaaattc aatactcaag gagataaaaa
    10921 atggaaaact taaacaataa tccacaaaaa attaatcaaa taaataagca aactaatttt
    10981 attggttttg aaaattttat agaacttgat aatcaagtta atcaaactaa agaaaaaagt
    11041 aa
//
