LOCUS       sequence-318194-       11341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(RBSmut)_Psyn-sgRNA500
FEATURES             Location/Qualifiers
     source          1..11341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        552..627
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2070..2084,2085..2090,2091..2107,2108..2114)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2090..2106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2122..2138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(2175..2201,2202..2204,2205..4691,4692..4694,
                     4695..6281)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6451..7242
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7365..7953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7854..7873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgacagcta gctcagtcct aggtataata ctagtggaag agccaaagtc aacacttata
       61 gacaaatatg atctttctaa ccttcacgaa atagcatcag ggggagcacc tttgtctaag
      121 gaagtaggag aggcagttgc aaagaggttt catttacccg gaattaggca aggatatggg
      181 ttaacagaaa ccacatctgc aattttaatt acacctgaag gagatgataa accaggagct
      241 gttgggaagg ttgttccttt ttttgaggct aaagttgttg atttagatac tggaaagact
      301 ttaggagtta atcaaagagg cgagttatgt gtaagaggac ctatgataat gtcaggatac
      361 gtaaataatc cagaagctac aaatgcattg atagataaag atggttggtt acactcaggc
      421 gatatagctt attgggacga agatgaacac ttttttatag ttgatagatt gaaatctttg
      481 attaagtata aaggttatca agtagctcca gcagaattgg aaagtatttt attgcaacac
      541 ccagctcttc cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      601 cttgaaaaag tggcaccgag tcggtgcttt ttttggcgcg ccaaataatg cccgtgtccc
      661 tgttgaatag ggacacggtt ttttaacaat ttttttattc ctaggcctta gtggccggta
      721 cccggggatc cttattactg gccgctttct agcctggata cttgtctggc tagttgcatt
      781 agagagtcag ctagagccct agggctagct gtttgtgtat tcggagccag tgtagttttt
      841 ctttttacta acgatacggg taataattga ttgcctttta cagcctgacc ttgagacaat
      901 tgcaaaagtc tgtccacaga ggtttgacct aataatctaa aatcttgttt gatagtagta
      961 agtggaggta tgtagcaact agaatcttct gtatcatcat aacccacaac tgaaatatca
     1021 gccccaactc taagtccact ttcagtaata gctcgcatag ctcctaaagc catttgatca
     1081 ttagcaacta acatagcagt agggacaatg ccttcattta acatttgcat tgtttgctga
     1141 aatcctgaca tagcagacca atctccttct ctttctgcta ttggttgtat ttgatttctt
     1201 gttaaatact tgtgccatcc tgctaatctc aatctggcac ttactgaact taagggacct
     1261 gcaagtagtg caatttgttg atggcctaat gcaacaaggt gttcaactcc taaccttgtt
     1321 ccatcttcgt gagaaaaaat tatagagttt attggggttt ggtccgacac atccaaaaac
     1381 aatgctggaa cattagtaca tgctgcttca actgcaatcg catcttggtc atcaagagga
     1441 taattaatta taagtccact aacacgttga gctaataaat tatgtactgc tgccttacaa
     1501 gcctctacac ctgatctctc aaccattgat acaacaacgc ttgcaccaag ttgatcagct
     1561 ctcgatttaa ttgcggctac aatttgacta ggcgcatgta atgcaagtga tgatgtggca
     1621 actccaatta aaagtgattg tttccccgca agttgctgag caacgcgatt tggtatataa
     1681 ttcaactctg ccatagctgc ttcaaccttc tctcttgttt ttgcagatac atgacttgct
     1741 tggttcacaa ctctcgaaac cgtttggtag ctaaccccgg catattcagc aacatcatac
     1801 aaagtaacag gtttcatgga tcattcctcc atgataaaat ttaaatttct gactttggca
     1861 aatccttttg cctgttaaaa agaatagcac aaaaattaaa aaaaaagaaa cgcaaatagc
     1921 aatagcatta aaaataatta ttattattta tttctttgat taaaatcaaa agcctttaat
     1981 gtattttctt gatctttcat gcgcttaacg gtaaaatcca aggtattaag ttcaccttcc
     2041 acttttctct ctctatcttc tctagaaaat cataaaaaat ttatttgctt tgtgagcgga
     2101 taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag
     2161 aagagaaatt acatatggat aagaaatact caataggctt agctatcggc acaaatagcg
     2221 tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg
     2281 gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg
     2341 gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga
     2401 agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata
     2461 gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc
     2521 atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct
     2581 atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt
     2641 tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc
     2701 ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat
     2761 ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat
     2821 tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg
     2881 gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt
     2941 ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag
     3001 ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt
     3061 tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc
     3121 ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa
     3181 aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa
     3241 aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta
     3301 tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg
     3361 aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact
     3421 tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca
     3481 atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg
     3541 cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat
     3601 ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga
     3661 caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg
     3721 agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa
     3781 aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa
     3841 atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg
     3901 atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg
     3961 atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct
     4021 tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac
     4081 ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt
     4141 atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg
     4201 gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc
     4261 tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac
     4321 aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag
     4381 gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc
     4441 cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa
     4501 attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc
     4561 ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc
     4621 tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt
     4681 atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg
     4741 tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag
     4801 tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta
     4861 agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt
     4921 ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg
     4981 atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga
     5041 ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac
     5101 gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg
     5161 ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt
     5221 atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat
     5281 atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag
     5341 agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata
     5401 aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca
     5461 agaaaacaga agtccagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt
     5521 cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata
     5581 gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga
     5641 agttaaaatc cgttaaagag ttactaggga tcacaattct ggaaagaagt tcctttgaaa
     5701 aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca
     5761 ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta
     5821 gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt
     5881 tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac
     5941 aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat
     6001 tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca
     6061 aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga
     6121 cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat
     6181 atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt
     6241 atgaaacacg cattgatttg agtcagctag gaggtgacta ataaaagctt gatcagatct
     6301 cagctttttt ttgaagtgcc tggcagtaag ttgaaaaaat ttgaaataaa cttggactat
     6361 gttaataaaa aaatacaaat taacgaacaa aaagatttga attattatat aatttagtta
     6421 aaagcaattt taaatgaggg aggtttccat atgagggaag cggtgatcgc cgaagtatcg
     6481 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     6541 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     6601 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     6661 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     6721 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     6781 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     6841 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     6901 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     6961 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     7021 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     7081 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     7141 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     7201 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgacgtcg ccggagcggt
     7261 cgagttctgg accgaccggc tcgggttctc atgaccaaaa tcccttaacg tgagttttcg
     7321 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7381 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7441 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7501 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7561 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7621 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7681 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7741 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7801 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7861 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7921 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7981 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcactaga tccccggcaa
     8041 gttcatcctt agggttttca tatctcttaa aaagtcaatt tctgcttgat taatttcttg
     8101 tggattattg tttttgcggt ttttcatttt ttatctcctt gagtattgaa ttttttctta
     8161 tatataaata tatagcaaaa actatttttg ccaacttttt tacaaaaaat tttttattaa
     8221 aacacttagg gctttgctaa attctctttt aaaagaactt agtaaagccc tatggattta
     8281 agaactgatt atacttacgt agtaaaaata ctacagatat taatagtata gaccatatca
     8341 aaatcataat caatttatat ttatctaaaa ttgtagtcca tacaaaatac aaaaaaatta
     8401 ggtttttcta aattctattt taaaagaact tagtaacacc taatctctaa attacttaca
     8461 aaattctaaa attagatttt tagttcttca tatttcttta aaagtttttt aagaaaatct
     8521 ttttgatttt caaaaatttc atgcatcata aaactagtaa atttggattt ttttttgtaa
     8581 aaatcataac tttcttgggt tttaagttga aatcttaatg gttttattgg attttgccta
     8641 gattttttta acgccggact ttctttatct tttataaaat tataagaatt tttaatacca
     8701 ttttctatta aataatcttc ttttataagc ccctcttgca gtgcagttgc taatcttaaa
     8761 tacttatacg cttgagtttt tgctacatca aagtctttta taaaatcaag aaaagtttta
     8821 taaccatcaa atttataata ttgattatct tttatttctt ttaaaatttt aatattttcc
     8881 accctgtgga atatttcttt tttgaaattg tcatttaact tttcctttag tgtcttgtaa
     8941 taagaaattt tactttcatt aatcattaaa ttttgttcaa cataagtatc tatctttttt
     9001 gttaaaactt taacaacacg ttttttttcc ataaaaattt tcctttattt ttgcaacatt
     9061 ttccttaatt gatataattg ttactatttt agatttttta atatccactt aagaattaat
     9121 ccctcactag aatatttttt attatagaat tcatatacat gtttttctaa atctataaat
     9181 ttatcaacaa ttgctttatt atagggtgta tctatctttt cttttcttaa taatcgtctc
     9241 atattcttta aataacaaca aacactgccg gttttaaacc taaattctat gtaatatatt
     9301 tttgaaaaag tatatacatt ttcaaccccg tttatttcat actttacaaa aacattattt
     9361 attggttttc tataaccata gtaaatgcct aaaaatttgt ccccgtcttt cataggatat
     9421 agataaagtt tttgaatctc tgatttatct aataattttt caaaaaaaat tatacaccgc
     9481 ttttgtttgt tactaactac tctatgcaat gcctttaata tttttgtgtg atatatattt
     9541 ttatcacctt ttttttcttt cagcaggaag atactttctt ttggaaattt aattttttgt
     9601 tcatttattc ttgcagcctt aagtccgatt ttcatattta atccttatat agcctttcta
     9661 tttaattctt ttaaactcaa taaacttttt tggtttttca ttagttctaa taattcgcaa
     9721 taatatgtgc aaaacacttt attgtattct attttctttt ggttattcaa atattctttt
     9781 ataattggct ttagaagttc aatatttact tcttttttta gttgttcaat aaggatattg
     9841 aaaatgtttg tttttaaatc ttgataattt tgttgagaat tttctttttt tctttcaatt
     9901 gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact ttcaataata
     9961 aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc taattgttta
    10021 gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc ctttaatttt
    10081 tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt tagtaattta
    10141 atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc aatatttaaa
    10201 ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt acatttattg
    10261 aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc ttttatatta
    10321 tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa tctatcttta
    10381 aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa gtatttatag
    10441 attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc ggtccccaaa
    10501 tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc taatttgtaa
    10561 aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac tatttctttt aaggttttca
    10621 ttaaagtaat agagtatgtt tttttgtgtg tatttcttat atgttttatt tatgtacgct
    10681 agtgttgaga taaggactac taatttatac tgtaatttat tgtagcaagt tgtggtttta
    10741 ctggttttgt ttatattaga taagttttcc atttatagct ccttgaatac cggatttttt
    10801 atctataaag tatatatatc acaattttga tttaaaagta aattcttttt ttaaaaatac
    10861 aaaaattttt tataaattat atagaccatg atattgtatt aatactaatt gattttaatt
    10921 taaggttgaa ctaaaataaa catagttttg tagaaaataa tttttcatta ttttgctact
    10981 tgatattgga tcgtaaaaat attagggctt tacaaagttc ttttaaaaga gaatttagta
    11041 aagccctaag tcttttaata aaaaattttt tgtaaaaaag ttggcaaaaa tagtttttgc
    11101 tatatattta tatataagaa aaaattcaat actcaaggag ataaaaaatg gaaaacttaa
    11161 acaataatcc acaaaaaatt aatcaaataa ataagcaaac taattttatt ggttttgaaa
    11221 attttataga acttgataat caagttaatc aaactaaaga aaaaagtaaa ttaagtaaga
    11281 taggtaagaa attacccgga ataagcagtc aagtctagtg cggccggaat tctaaagatc
    11341 t
//
