LOCUS       sequence-318195-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding RFP (for example, to normalize 
            plasmid-based protein expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pLEX307 RFP
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..675
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1..675
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(131..149)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     593..616
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
       61 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      121 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      241 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      361 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      421 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
      481 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
      541 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
      601 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
      661 cactccaccg gcgcc
//
