LOCUS       sequence-318197-         363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding human NLRP1 CARD with a 
            C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pcDNA3.1 6A NLRP1 CARD-FLAG WT
FEATURES             Location/Qualifiers
     source          1..363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(305..329)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             337..360
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggatgccc cgcagttgct gcactttgtg gaccagtatc gagagcagct gatagcccga
       61 gtgacatcgg tggaggttgt cttggacaaa ctgcatggac aggtgctgag ccaggagcag
      121 tacgagaggg tgctggctga gaacacgagg cccagccaga tgcggaagct gttcagcttg
      181 agccagtcct gggaccggaa gtgcaaagat ggactctacc aagccctgaa ggagacccat
      241 cctcacctca ttatggaact ctgggagaag ggcagcaaaa agggactcct gccactcagc
      301 agcgacccag ctttcttgta caaagtggtt gatatcgact acaaggacga tgacgacaag
      361 tag
//
