LOCUS       sequence-318205-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding human NLRP1 UPA-CARD 
            (filament-deficient mutant) with a C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(788..812)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             820..843
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtccccct tgggagtcct cctgaaaatg atccataatg ccctgcgctt cattcccgtc
       61 acctctgtgg tgttgcttta ccaccgcgtc catcctgagg aagtcacctt ccacctctac
      121 ctgatcccaa gtgactgctc cattcggaag gccatagatg atctagaaat gaaattccag
      181 tttgtgcgaa tccacaagcc acccccgctg accccacttt atatgggctg tcgttacact
      241 gtgtctgggt ctggttcagg gatgctggaa atactcccca aggaactgga gctctgctat
      301 cgaagccctg gagaagacca gctgttctcg gagttctacg ttggccactt gggatcaggg
      361 atcaggctgc aagtgaaaga caagaaagat gagactctgg tgtgggaggc cttggtgaaa
      421 ccaggagatc tcatgcctgc aactactctg atccctccag cccgcatagc cgtaccttca
      481 cctctggatg ccccgcagtt gctgcacttt gtggaccagt atcgagagca gctgatagcc
      541 cgagtgacat cggtggaggt tgtcttggac aaactgcatg gacaggtgct gagccaggag
      601 cagtacgaga gggtgctggc tgagaacacg aggcccagcc agatggagaa gctgttcagc
      661 ttgagccagt cctgggaccg gaagtgcaaa gatggactct accaagccct gaaggagacc
      721 catcctcacc tcattatgga actctgggag aagggcagca aaaagggact cctgccactc
      781 agcagcgacc cagctttctt gtacaaagtg gttgatatcg actacaagga cgatgacgac
      841 aagtag
//
