LOCUS       sequence-318220-         303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding codon-optimized human NLRP1 
            CARD (dimer interface mutation) with a C-terminal mCherry tag for 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry...
FEATURES             Location/Qualifiers
     source          1..303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggacgctc ctcagctgct gcatttcgtg gaccagtacc gggagcagct gatcgctaga
       61 gtgacctccg tggaagtggt gctggacaag ctgcacggac aggtgctgag tcaggagcag
      121 tacgagaggg tgctggcaga gaacaccagg cctagccaga tgcggaagct gttcagcctg
      181 agccagtctt gggacaggaa gtgcaaggac ggcctgtacc aggctctgaa ggagacacac
      241 ccccacctga tcatggagct cgcggagaag ggaagcaaga agggcctgct gcctctgtct
      301 agc
//
