LOCUS       sequence-318227-         291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector encoding a HRV 3C-cleavable 
            His-MBP-tagged NLRP1 CARD .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pDB.His.MBP.3C NLRP1 CARD WT
FEATURES             Location/Qualifiers
     source          1..291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctgcact ttgtggacca gtatcgagag cagctgatag cccgagtgac atcggtggag
       61 gttgtcttgg acaaactgca tggacaggtg ctgagccagg agcagtacga gagggtgctg
      121 gctgagaaca cgaggcccag ccagatgcgg aagctgttca gcttgagcca gtcctgggac
      181 cggaagtgca aagatggact ctaccaagcc ctgaaggaga cccatcctca cctcattatg
      241 gaactctggg agaagggcag caaaaaggga ctcctgccac tcagcagctg a
//
