LOCUS       sequence-318235-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding CARD8 UPA-CARD 
            (filament-deficient mutation) with a C-terminal mCherry tag for 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctctgatgg gcatcctgct gcggatcgcc agtgggactc gcctctccat ccccatcact
       61 tccaacacat tgatctatta tcacccccac cccgaagata ttaagttcca cttgtacctt
      121 gtccccagcg acgccttgct aacaaaggcg atagatgatg aggaagatcg cttccatggt
      181 gtgcgcctgc agacttcgcc cccaatggaa cccctgaact ttggttccag ttatattgtg
      241 tctaattctg ctaacctgaa agtaatgccc aaggagttga aattgtccta caggagccct
      301 ggagaaattc agcacttctc aaaattctat gctgggcaga tgaaggaacc cattcaactt
      361 gagattactg aaaaaagaca tgggactttg gtgtgggata ctgaggtgaa gccagtggat
      421 ctccagcttg tagctgcatc agcccctcct cctttctcag gtgcagcctt tgtgaaggag
      481 aaccaccggc aactccaagc caggatgggg gacctgaaag gggtgctcga tgatctccaa
      541 gacaatgagg ttcttactga gaatgagaag gagctggtgg agcaggaaaa gacagagcag
      601 agcaagaatg aggccttgct gagcatggtg gagaagaaag gggacctggc cctggacgtg
      661 ctcttcagaa gcattagtga aagggaccct tacctcgtgt cctatcttag acagcagaat
      721 ttg
//
