LOCUS       sequence-318239-         339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector encoding human CARD8 CARD with a 
            C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pcDNA3.1 6A CARD8 CARD-FLAG WT
FEATURES             Location/Qualifiers
     source          1..339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(281..305)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             313..336
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcctcctt tctcaggtgc agcctttgtg aaggagaacc accggcaact ccaagccagg
       61 atgggggacc tgaaaggggt gctcgatgat ctccaagaca atgaggttct tactgagaat
      121 gagaaggagc tggtggagca ggaaaagaca cggcagagca agaatgaggc cttgctgagc
      181 atggtggaga agaaagggga cctggccctg gacgtgctct tcagaagcat tagtgaaagg
      241 gacccttacc tcgtgtccta tcttagacag cagaatttgg acccagcttt cttgtacaaa
      301 gtggttgata tcgactacaa ggacgatgac gacaagtag
//
