LOCUS       sequence-318240-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector encoding a HRV 3C-cleavable 
            His-MBP-tagged CARD8 CARD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pDB.His.MBP.3C CARD8 UPA-CARD WT
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtctctga tgggcatcct gctgcggatc gccagtggga ctcgcctctc catccccatc
       61 acttccaaca cattgatcta ttatcacccc caccccgaag atattaagtt ccacttgtac
      121 cttgtcccca gcgacgcctt gctaacaaag gcgatagatg atgaggaaga tcgcttccat
      181 ggtgtgcgcc tgcagacttc gcccccaatg gaacccctga actttggttc cagttatatt
      241 gtgtctaatt ctgctaacct gaaagtaatg cccaaggagt tgaaattgtc ctacaggagc
      301 cctggagaaa ttcagcactt ctcaaaattc tatgctgggc agatgaagga acccattcaa
      361 cttgagatta ctgaaaaaag acatgggact ttggtgtggg atactgaggt gaagccagtg
      421 gatctccagc ttgtagctgc atcagcccct cctcctttct caggtgcagc ctttgtgaag
      481 gagaaccacc ggcaactcca agccaggatg ggggacctga aaggggtgct cgatgatctc
      541 caagacaatg aggttcttac tgagaatgag aaggagctgg tggagcagga aaagacacgg
      601 cagagcaaga atgaggcctt gctgagcatg gtggagaaga aaggggacct ggccctggac
      661 gtgctcttca gaagcattag tgaaagggac ccttacctcg tgtcctatct tagacagcag
      721 aatttgtag
//
