LOCUS       sequence-318246-         279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector encoding a HRV 3C-cleavable 
            His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C 
            mutation for cysteine labelling applications).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif 
            H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
  TITLE     Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 
            inflammasomes.
  JOURNAL   Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y.
  PUBMED    33420033
REFERENCE   2  (bases 1 to 279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-8"; volume: "12(1):189. doi"; pages: " 
            10.1038/s41467-020-20320-y"
COMMENT     Sequence Label: pDB.His.MBP.3C CARD8 CARD A452C E490R
FEATURES             Location/Qualifiers
     source          1..279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctttct caggtgcatg ctttgtgaag gagaaccacc ggcaactcca agccaggatg
       61 ggggacctga aaggggtgct cgatgatctc caagacaatg aggttcttac tgagaatgag
      121 aaggagctgg tgcggcagga aaagacacgg cagagcaaga atgaggcctt gctgagcatg
      181 gtggagaaga aaggggacct ggccctggac gtgctcttca gaagcattag tgaaagggac
      241 ccttacctcg tgtcctatct tagacagcag aatttgtag
//
