LOCUS       sequence-31830-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C
  TITLE     Mms2-Ubc13 covalently bound to ubiquitin reveals the structural 
            basis of linkage-specific polyubiquitin chain formation.
  JOURNAL   Nat Struct Mol Biol. 2006 Oct . 13(10):915-20.
  PUBMED    16980971
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2006 Oct . 13(10):915-20."
COMMENT     Sequence Label: MBP-His6-TEV-MMS2
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..19
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             29..52
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(521..538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(521..537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(716..733)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cctcgggatc gagggaagga tcagcggcca ccaccaccac caccaccacc acgactacga
       61 catcccctcc tccgagaacc tgtacttcca gggatccatg tcaaaagtac caagaaattt
      121 taggttgtta gaagaattag aaaagggtga aaaagggttt ggtccagaat catgtagtta
      181 tggtttagct gatagcgacg atattaccat gaccaaatgg aacggcacta ttttagggcc
      241 ccctcatagt aatcatgaaa acagaatcta ttccctttct atagattgtg gcccaaacta
      301 tccagattct cccccaaagg tcactttcat ttccaagata aatctaccat gcgtcaatcc
      361 aactacgggg gaggtccaaa ctgacttcca caccttacgt gactggaaaa gagcctacac
      421 catggaaact ttattacttg atcttagaaa ggaaatggca accccagcga acaagaaatt
      481 gcgtcaaccg aaggaaggag aaacctttta gaagcttggc actggccgtc gttttacaac
      541 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      601 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      661 gcctgaatgg cgaatggcag cttggctgtt ttggcggatg agataagatt ttcagcctga
      721 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
      781 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
      841 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaaacgaaa
      901 gg
//
