LOCUS       sequence-31831-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C
  TITLE     Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic 
            enzyme.
  JOURNAL   Nat Struct Mol Biol. 2006 Jul . 13(7):661-2.
  PUBMED    16783373
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2006 Jul . 13(7):661-2."
COMMENT     Sequence Label: Nampt-PET28a
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatgaatgc tgcggcagaa
      121 gccgagttca acatcctgct ggccaccgac tcgtacaagg ttactcacta taaacaatac
      181 ccacccaaca caagcaaagt ttattcctac tttgaatgcc gtgaaaagaa gacagaaaac
      241 tccaaagtaa ggaaggtgaa atacgaggaa acagtatttt atgggttgca gtacattctt
      301 aataagtact taaaaggtaa agtagtgacc aaagagaaaa tccaggaggc caaagaagtg
      361 tacagagaac atttccaaga tgatgtcttt aacgaaagag gatggaacta catccttgag
      421 aaatacgatg gtcatctccc gattgaagta aaggctgttc ccgagggctc tgtcatcccc
      481 agagggaacg tgctgttcac agtggaaaac acagacccag agtgctactg gcttaccaat
      541 tggattgaga ctattcttgt tcagtcctgg tatccaatta cagtggccac aaattccaga
      601 gaacagaaga aaatactggc caaatatttg ttagaaacct ctggtaactt agatggtctg
      661 gaatacaagt tacatgactt tggttacaga ggagtctctt cgcaagagac tgctggcata
      721 ggggcatctg ctcatttggt taactttaaa ggaacagata ctgtggcggg aattgctcta
      781 attaaaaaat actatgggac aaaagatcct gttccaggct attctgttcc agcagcagag
      841 cacagtacca taacggcttg ggggaaagac atgagaagat gctttga
//
