LOCUS       sequence-318311-        8835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin 
            resistant. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Rovira-Clave X, Drainas AP, Jiang S, Bai Y, Baron M, Zhu B, Dallas 
            AE, Lee MC, Chu TP, Holzem A, Ayyagari R, Bhattacharya D, McCaffrey 
            EF, Greenwald NF, Markovic M, Coles GL, Angelo M, Bassik MC, Sage J,
            Nolan GP
  TITLE     Spatial epitope barcoding reveals clonal tumor patch behaviors.
  JOURNAL   Cancer Cell. 2022 Nov 14;40(11):1423-1439.e11. doi: 
            10.1016/j.ccell.2022.09.014. Epub 2022 Oct 13.
  PUBMED    36240778
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2022.09.014"; journalName: "Cancer Cell"; date: 
            "2022-11-14- 14"; volume: "40"; issue: "11"; pages: "1423-1439.e11"
COMMENT     Sequence Label: pEpicVector3mC_Puro-T2A-mCherry-HA-FLAG-AU1
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1210..1806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1210..1229)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1706..1726
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1816..1869
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1870..2577
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2015..2033)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2283..2302
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2608..2634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2608..2634
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2635..2658
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2680..2704)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2722..3310
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2775..2795)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3193..3204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3398..3631
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3703..3824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3740..3759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3794..3813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3864..3999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4019..4038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4020..4038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4048..4065)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4048..4064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4057..4079)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4206..4661
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4293..4312)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4503..4524
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4687..4791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4792..4860,4861..5652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5010..5029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5823..6411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6312..6331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6565..6582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6699..6720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6735..6740,6741..6758,6759..6765)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6773..6789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6778..6800
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6797..6813
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6797..6813
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6832..6852
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6834..6852
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6880..7106
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7107..7287
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7334..7459
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7952..8185
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8370..8414
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8680..8797
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    8815..8835
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac aagtttgtac aaaaaagcag
     1201 gctgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     1261 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     1321 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     1381 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     1441 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     1501 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     1561 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     1621 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     1681 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     1741 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     1801 ggtgccggaa gcggagaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat
     1861 cccggcccca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1921 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1981 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2041 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2101 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2221 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2281 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2341 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2401 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2461 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2521 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagggt
     2581 ggcagcggcg gcggcggctc tggcggctac ccctacgacg tgcccgacta cgccgactac
     2641 aaagacgatg acgacaagga cacctacaga tacatctaaa cccagctttc ttgtacaaag
     2701 tggtgataat cgaattccga taatcaacct ctggattaca aaatttgtga aagattgact
     2761 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2821 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2881 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2941 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3001 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3061 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3121 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3181 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3241 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3301 gcctccccgc atcgggaatt cccgcggttc gctttaagac caatgactta caaggcagct
     3361 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3421 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3481 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3541 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3601 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     3661 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3721 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3781 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3841 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3961 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     4021 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     4081 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4141 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4201 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4261 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4321 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4381 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4441 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4501 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4561 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4621 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4681 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4801 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4861 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4921 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4981 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5041 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5101 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5161 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5221 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5281 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5341 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5401 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5461 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5521 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5581 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5641 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5701 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6241 gagagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6661 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6721 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6781 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6841 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     6901 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6961 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     7021 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     7081 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     7141 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7201 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7261 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     7321 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     7381 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     7441 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     7501 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     7561 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     7621 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     7681 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7741 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7801 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     7861 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7921 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7981 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     8041 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     8101 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     8161 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     8221 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     8281 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     8341 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     8401 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     8461 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     8521 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     8581 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     8641 ccattcgatt agtgaacgga tctcgacggt atcgctagct tttaaaagaa aaggggggat
     8701 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8761 agaattacaa aaacaaatta caaaaattca aaattttact agtgattatc ggatcaactt
     8821 tgtatagaaa agttg
//
