LOCUS       sequence-31833-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Feeser EA, Wolberger C
  TITLE     Structural and functional studies of the Rap1 C-terminus reveal 
            novel separation-of-function mutants.
  JOURNAL   J Mol Biol. 2008 Jul 11. 380(3):520-31.
  PUBMED    18538788
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2008 Jul 11. 380(3):520-31."
COMMENT     Sequence Label: pMal672H
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="recognition and cleavage site for Genenase(TM) I,
                     a subtilisin BPN' variant engineered for increased 
                     substrate specificity (Carter and Wells, 1987)"
                     /label=Genenase(TM) I site
                     /translation="PGAAHY"
     protein_bind    48..72
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             561..578
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             579..596
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(600..624)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(672..689)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(672..688)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(681..703)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cacataacaa taacaacaac ctcgggccgg gtgcggcaca ctacatcaca agtttgtaca
       61 aaaaagcagg cttcgaaaat ttatattttc aaggtgctat aagaagcgat ttttcgaatg
      121 aagatatata tgataatatt gatcctgata ccataagctt tccaccaaaa attgcaacaa
      181 cagatctttt cctgccattg ttctttcatt ttggcagtac gagacaattt atggataaac
      241 ttcatgaagt tatttcagga gattatgagc catcacaggc tgaaaaactg gtacaggatc
      301 tttgcgatga aactggtata cgtaaaaatt tcagtacgag catattgacg tgtttatccg
      361 gggatctgat ggtctttcct cgctatttct tgaacatgtt taaggacaat gttaatcctc
      421 ctcccaacgt tcctggtatt tggacacatg atgacgacga atcgctaaaa agcaatgatc
      481 aagaacaaat aaggaaactg gttaaaaagc atggaactgg tagaatggaa atgaggaaaa
      541 gattttttga gaaggacctg ttagttcccc ggggttctca ccaccaccac caccactaga
      601 cccagctttc ttgtacaaag tggtgatgta gaattcggat cctctagagt cgacctgcag
      661 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      721 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggnc
      781 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc agctnggctg
      841 ttttggcgga tgagataaga ttttc
//
