LOCUS       sequence-318333-       11495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive control for FRET in plant ER / nuclear envelope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 11495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: p35S::SP-mCherry-GFP-HDEL
FEATURES             Location/Qualifiers
     source          1..11495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    60..84
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1384..2013
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2447..3514
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3580..3774
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4010..4032)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4118..4258
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4173..4192
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4273..4290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4444..5032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4524..4543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5276..6067)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6595..6619
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6712..6967)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6987..7538)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7580..7759)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(join(8121..8834,8835..8837,8838..8840))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8124..8834,8835..8837,8838..8840))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    8848..8872
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(8889..9599,9600..9602,9603..9605))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8889..9599,9600..9602,9603..9605))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(9621..10328)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9896..9915)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(10395..10419)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(10518..10862)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10554..10574)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cttagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
       61 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat
      121 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
      181 gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct
      241 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc
      301 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa
      361 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg
      421 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg
      481 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg
      541 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga
      601 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc
      661 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg
      721 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca
      781 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc
      841 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc
      901 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac
      961 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg
     1021 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag
     1081 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc
     1141 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct
     1201 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     1261 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     1321 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     1381 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     1441 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     1501 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     1561 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     1621 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     1681 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     1741 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     1801 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     1861 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     1921 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     1981 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     2041 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     2101 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     2161 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     2221 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     2281 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     2341 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
     2401 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc
     2461 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     2521 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     2581 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     2641 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     2701 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     2761 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     2821 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     2881 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     2941 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     3001 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     3061 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     3121 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
     3181 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     3241 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     3301 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     3361 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     3421 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     3481 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
     3541 tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg
     3601 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
     3661 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
     3721 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
     3781 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
     3841 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
     3901 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
     3961 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     4021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     4081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     4141 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     4201 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     4261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4861 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5101 ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat
     5161 aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaatc
     5221 gcttgagtta acgccggcga agcggcgtcg gcttgaacga atttctagct agacattatt
     5281 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
     5341 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
     5401 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     5461 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     5521 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     5581 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     5641 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     5701 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     5761 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     5821 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     5881 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     5941 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     6001 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     6061 cccccatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     6121 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     6181 tagactgtac cccaaaaaaa catgtcataa caagaagcca tgaaaaccgc cactgcgccg
     6241 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgacggcag ttacgctact
     6301 tgcattacag cttacgaacc gaacgaggct tatgtccact gggttcgtgc ccgaattgat
     6361 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
     6421 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
     6481 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
     6541 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
     6601 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
     6661 tttaatgtac tgaattaacg ccgaattgaa ttatcagctt gcatgccggt cgatctagta
     6721 acatagtaga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     6781 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     6841 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     6901 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     6961 aacgatctgc ttgactctag gggtcatcag atttcggtga cgggcaggac cggacggggc
     7021 ggcaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     7081 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     7141 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     7201 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     7261 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca gggacccgcg
     7321 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     7381 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     7441 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     7501 gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatcccct cgatcgagtt
     7561 gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc
     7621 atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa
     7681 taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctcagtggc
     7741 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
     7801 cgggggtcat aacgtgactc ccttaattct catgtatgat aattcgaggg tacccgggga
     7861 tcctctagag ggcccgacgt cgcatgcctg caggtcactg gattttggtt ttaggaatta
     7921 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     7981 ctcaacacat gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca
     8041 cattattctg gagaaaaata gagagagata gatttgtaga gagagactgg tgatttttgc
     8101 ggactctagc atggccgcgg ttacttgtac agctcgtcca tgccgagagt gatcccggcg
     8161 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     8221 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     8281 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     8341 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     8401 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     8461 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     8521 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     8581 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     8641 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     8701 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     8761 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     8821 tcctcgccct tgctcaccat tgatatcacc actttgtaca agaaagctgg gtgtcataat
     8881 tcatcatgct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     8941 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     9001 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     9061 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     9121 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     9181 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     9241 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     9301 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     9361 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     9421 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     9481 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     9541 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     9601 accatacctc cactgccacc cttgtacagc tcgtccatgc cgccggtgga gtggcggccc
     9661 tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac
     9721 ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc
     9781 ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc
     9841 ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc
     9901 ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg
     9961 aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag
    10021 ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg
    10081 tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc
    10141 caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg
    10201 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
    10261 atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg
    10321 ctcaccatgg ctggattggg caggagatag agggaaagag ctaagaagag agcgagtgtg
    10381 aaggctttca ttgtagcctg cttttttgta caaacttgtg atatcactag tgcggccgcc
    10441 tgcaggtcga ctagaatagt aaattgtaat gttgtttgtt gtttgttttg ttgtggtatt
    10501 gttgtaaaaa taccggagtc ctctccaaat gaaatgaact tccttatata gaggaagggt
    10561 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    10621 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    10681 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    10741 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    10801 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    10861 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    10921 cgtgctccac catgttgacg aagattttct tcttgtcatt gagtcgtaaa agactctgta
    10981 tgaactgttc gccagtcttc acggcgagtt ctgttagatc ctcgatctga atttttgact
    11041 ccatggcctt tgattcagta ggaactactt tcttagagac tccaatctct attacttgcc
    11101 ttggtttatg aagcaagcct tgaatcgtcc atactggaat agtacttctg atcttgagaa
    11161 atatatcttt ctctgtgttc ttgatgcagt tagtcctgaa tcttttgact gcatctttaa
    11221 ccttcttggg aaggtatttg atctcctgga gattattact cgggtagatc gtcttgatga
    11281 gacctgccgc gtaggcctct ctaaccatct gtgggtcagc attctttctg aaattgaaga
    11341 ggctaatctt ctcattatcg gtggtgaaca tggtatcgtc accttctccg tcgaactttc
    11401 ttcctagatc gtagagatag agaaagtcgt ccatggtgat ctccggggca aaggagatca
    11461 gcttggctct agtcgaccat atgggagagc tcaag
//
