LOCUS       sequence-318337-       11091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeted integration of SARS-CoV-2 spike HexaPro variant into the 
            AAVS1 genomic safe harbor locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Rivest JF, Goupil C, Doyon Y
  TITLE     Artisanal production of prefusion-stabilized SARS-CoV-2 spikes
  JOURNAL   protocols.io 2021
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "protocols.io 2021"
COMMENT     Sequence Label: AAVS1_puro_CAG_HexaPro
FEATURES             Location/Qualifiers
     source          1..11091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    320..1123
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1130..1155
                     /label=SA
                     /note="splice acceptor site"
     CDS             1179..1232
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1236..1832
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1729..1749
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1848..1969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1859..1878)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1913..1932
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        2360..2663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2666..2941
                     /label=chicken beta-actin promoter
     intron          2942..3954
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3878..3900
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             7762..7785
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7789..7812
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             7819..7842
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7882..7905
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    7944..8168
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8188..9024
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(9101..9118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9272..9860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9352..9371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10031..10822,10823..10891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10654..10673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10892..10996)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11064..11082
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagaa ttcgcccttt gctttctctg accagcattc tctcccctgg gcctgtgccg
      361 ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc
      421 cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc
      481 tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc
      541 tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc
      601 catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc
      661 tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt
      721 gtaggcctgc atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt
      781 tagccacctc tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg
      841 ggtcacctct cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg
      901 tgctagctct tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc
      961 ttcttcctcc aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg
     1021 gatcctgtgt ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg
     1081 gttctgggta cttttatctg tcccctccac cccacagtgg ggcaagcttc tgacctcttc
     1141 tcttcctccc acagggcctc gagagatctg gcggcggaga gggcagagga agtcttctaa
     1201 catgcggtga cgtggaggag aatcccggcc ctagcaccga gtacaagccc acggtgcgcc
     1261 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     1321 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     1381 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     1441 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     1501 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     1561 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     1621 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     1681 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     1741 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     1801 cctggtgcat gacccgcaag cccggtgcct gatctagaca gacatgataa gatacattga
     1861 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     1921 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     1981 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aatgcatcca
     2041 cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     2101 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     2161 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     2221 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     2281 cggaagccgc acgtctcact agttattaat agtaatcaat tacggggtca ttagttcata
     2341 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2401 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2461 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
     2521 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2581 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2641 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
     2701 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     2761 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     2821 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     2881 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     2941 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
     3001 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     3061 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
     3121 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
     3181 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
     3241 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     3301 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     3361 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     3421 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     3481 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     3541 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     3601 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     3661 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     3721 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     3781 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     3841 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     3901 ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     3961 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattgcg gccgtctcag
     4021 gccgagttcg gtaccgccac catgttcgtg ttcctggtgc tcctgcctct ggtgagcagc
     4081 cagtgcgtga acctgaccac ccgaacccag ctcccaccag cctacaccaa cagctttaca
     4141 cggggcgtgt actaccctga caaggtgttc agatctagcg tcctgcacag cactcaggac
     4201 ctcttcctgc cgttcttcag caacgtgaca tggttccacg ccatccacgt gagcggcaca
     4261 aacggaacca agcggtttga taaccccgtc ctgccattca atgatggagt ttacttcgcc
     4321 agtaccgaga agagtaacat catccggggc tggatcttcg gcaccaccct ggatagcaaa
     4381 acacagagcc tcctgatcgt gaacaatgcc acgaacgtcg tgatcaaggt gtgcgagttc
     4441 cagttttgca atgatccttt cctgggtgtg tactaccaca agaacaacaa gagctggatg
     4501 gaaagcgagt tcagagtcta cagcagcgcc aacaactgca cattcgagta cgtctctcag
     4561 ccttttctga tggaccttga ggggaaacaa ggcaacttca agaacctgag agaattcgtg
     4621 ttcaagaaca tcgacggcta cttcaaaatc tactccaagc acacacccat caacctggtc
     4681 cgggacctcc ctcagggctt cagcgccctg gaacccctgg tcgacctgcc cataggcatc
     4741 aacataacgc ggttccaaac cctgctggcc ctgcatagat cctacctgac tcctggcgac
     4801 agcagcagcg gatggaccgc cggagctgca gcctactatg tgggctacct gcaacctaga
     4861 accttcctgc tgaagtacaa cgagaacggc acaatcacag acgccgtcga ctgcgccctg
     4921 gaccctctct ctgagacaaa gtgcaccctg aagtccttca ccgtggaaaa gggcatctac
     4981 cagaccagca acttccgggt gcagcctaca gagagcatcg tgcgatttcc aaacattacc
     5041 aacctctgcc ccttcggcga ggtgtttaac gccacaagat ttgcctccgt ttacgcctgg
     5101 aatagaaaga gaatcagcaa ttgtgtggcc gactactccg tgctgtataa cagcgcctct
     5161 ttcagcacct tcaagtgcta cggcgtttcc ccaacaaagc tgaatgacct gtgcttcacc
     5221 aacgtgtacg ccgactcctt cgtaattaga ggcgatgagg tgcggcagat cgcaccaggc
     5281 cagaccggta agatcgctga ctacaactat aagctgcctg atgattttac aggctgcgtg
     5341 atcgcctgga actctaacaa cctggatagc aaggtgggcg gcaactacaa ctacctgtac
     5401 cggctgtttc gcaagtctaa cctgaaacct ttcgagagag acatctccac agagatctac
     5461 caggccggtt ctacaccttg taacggggtg gaaggcttca actgttactt ccctctgcaa
     5521 agctacggct tccagcctac caatggagtc ggctaccagc cataccgggt ggtcgtgctg
     5581 tccttcgagt tactccacgc ccccgccacc gtctgcggtc ctaagaagtc caccaatctg
     5641 gttaagaaca aatgcgtgaa cttcaacttc aacggcctga ccgggaccgg cgtgctgacc
     5701 gaaagcaaca aaaagttcct ccccttccag cagttcggcc gtgatatcgc tgacaccaca
     5761 gatgccgtca gagatccaca gaccctggaa atcctggata ttacaccctg ctccttcgga
     5821 ggagtttctg tgatcacccc cgggaccaat accagcaacc aggtggctgt gctgtaccaa
     5881 gatgttaact gcaccgaggt tcctgtggcc atccacgccg atcagctgac acctacttgg
     5941 agagtgtact ccactggctc caatgtgttc cagaccaggg ccggatgtct gatcggcgcc
     6001 gagcacgtga ataacagtta cgagtgcgac atccctatcg gcgccggcat ctgtgccagc
     6061 taccagaccc agacaaacag ccctgggtct gcttcctctg tagctagcca gagcatcatc
     6121 gcctacacca tgagcctggg cgcagagaac agcgtggcct attccaacaa ctctatcgcc
     6181 attcccacca actttacaat tagcgtcaca acagagatcc tgcccgtgag catgaccaag
     6241 accagcgtgg actgtacaat gtacatctgt ggcgacagca ctgaatgcag caacctgctg
     6301 ctgcaatacg gctccttttg cacccaactg aaccgggcgc tgaccggaat cgccgtggaa
     6361 caggacaaaa atacccagga ggtgttcgcc caagtgaagc agatctacaa gaccccacct
     6421 atcaaggact tcggcggctt taactttagc cagattctcc ctgatccttc taagcctagc
     6481 aagcggagcc ctatcgagga tctgctgttc aacaaggtca ccctggccga tgccggcttt
     6541 atcaaacagt atggcgattg cctgggcgac atagccgcca gagatctgat ctgcgcccag
     6601 aaattcaacg gcctgacagt tctcccacct ctgctgaccg acgagatgat cgctcagtac
     6661 acctctgccc tgctggctgg caccatcaca tctgggtgga catttggcgc cggccctgcc
     6721 ctgcagatcc cctttcccat gcagatggcc tatagattca acggaatcgg cgtgacccag
     6781 aacgtgctgt atgaaaacca gaagctgatc gctaaccagt tcaattctgc catcggcaag
     6841 atccaggact ccctctcctc tacccctagc gccctgggca aactgcagga cgtggtgaat
     6901 cagaacgccc aagccctgaa caccctggtg aagcagctca gcagcaattt tggcgccatc
     6961 agctctgtgc tgaacgatat cctgtctaga ctggaccctc cagaagccga agtccagatc
     7021 gatagactga tcacaggcag actgcagtcc ctgcaaacct acgtgaccca acagctgatc
     7081 agggccgctg aaataagagc cagcgccaat ctcgccgcta ccaagatgtc cgagtgtgtg
     7141 ctgggacagt ctaaacgcgt tgacttctgc ggcaaaggct atcacctgat gagcttcccc
     7201 cagagcgcgc cgcacggcgt ggtgttcctg catgtgacat acgtgcctgc ccaagagaag
     7261 aatttcacaa ccgcccctgc catctgccac gacggcaagg cccacttccc aagagagggc
     7321 gttttcgttt ccaatggcac acactggttc gtgacacaaa gaaacttcta cgaaccccag
     7381 attatcacca ccgacaacac cttcgtgagt ggcaattgtg acgtggtcat cggaatcgtg
     7441 aacaacacag tgtacgaccc tctgcaacct gagctggact cttttaagga agagctggac
     7501 aagtacttta aaaaccacac cagccccgat gtggacctgg gcgacatcag tggcattaac
     7561 gccagcgtgg tgaacatcca aaaggaaatc gacagactga acgaggtggc caagaacctg
     7621 aacgagtccc tgatcgacct gcaggagctc ggcaaatacg agcagggatc cggatacatc
     7681 cccgaggccc ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtactgctg
     7741 agcacattcc tgggcagatc cctggaggtg ctgttccagg gcccaggcca tcaccaccat
     7801 caccaccatc atagcgcctg gtcccacccc cagttcgaga agggcggcgg tagtggaggg
     7861 ggcggatctg gcggctcagc ttggagccac ccccagttcg aaaagtgata atgactcgag
     7921 cggccgcgtt taaaccctgc aggctgtgcc ttctagttgc cagccatctg ttgtttgccc
     7981 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8041 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8101 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     8161 ctctatgggt cgacagtact aagctttact agggacagga ttggtgacag aaaagcccca
     8221 tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag
     8281 gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga
     8341 acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg
     8401 gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta
     8461 ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg
     8521 tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat
     8581 aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt
     8641 cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg
     8701 ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta
     8761 ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt
     8821 aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc
     8881 taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag
     8941 gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc
     9001 tacaggggtt cctggctctg ctctaagggc gaattctgca ttaatgaatc ggccaacgcg
     9061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    11041 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
