LOCUS       sequence-318339-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi rodA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S(-10AC1)_Psyn-sgRNArodA
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..650
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        672..747
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(2190..2204,2205..2210,2211..2227,2228..2234)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    2210..2226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2242..2258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2277..2288
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(2295..2321,2322..2324,2325..4811,4812..4814,
                     4815..6401)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6571..7362
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7485..8073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7974..7993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagttgtggt tttactggtt ttgtttatat tagataagtt ttccatttat agctccttga
       61 ataccggatt ttttatctat aaagtatata tatcacaatt ttgatttaaa agtaaattct
      121 ttttttaaaa atacaaaaat tttttataaa ttatatagac catgatattg tattaatact
      181 aattgatttt aatttaaggt tgaactaaaa taaacatagt tttgtagaaa ataatttttc
      241 attattttgc tacttgatat tggatcgtaa aaatattagg gctttacaaa gttcttttaa
      301 aagagaattt agtaaagccc taagtctttt aataaaaaat tttttgtaaa aaagttggca
      361 aaaatagttt ttgctatata tttatatata agaaaaaatt caatactcaa ggagataaaa
      421 aatggaaaac ttaaacaata atccacaaaa aattaatcaa ataaataagc aaactaattt
      481 tattggtttt gaaaatttta tagaacttga taatcaagtt aatcaaacta aagaaaaaag
      541 taaattaagt aagataggta agaaattacc cggaataagc agtcaagtct agtgcggccg
      601 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt gttaatctac
      661 ctaatatacc agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      721 cttgaaaaag tggcaccgag tcggtgcttt ttttggcgcg ccaaataatg cccgtgtccc
      781 tgttgaatag ggacacggtt ttttaacaat ttttttattc ctaggcctta gtggccggta
      841 cccggggatc cttattactg gccgctttct agcctggata cttgtctggc tagttgcatt
      901 agagagtcag ctagagccct agggctagct gtttgtgtat tcggagccag tgtagttttt
      961 ctttttacta acgatacggg taataattga ttgcctttta cagcctgacc ttgagacaat
     1021 tgcaaaagtc tgtccacaga ggtttgacct aataatctaa aatcttgttt gatagtagta
     1081 agtggaggta tgtagcaact agaatcttct gtatcatcat aacccacaac tgaaatatca
     1141 gccccaactc taagtccact ttcagtaata gctcgcatag ctcctaaagc catttgatca
     1201 ttagcaacta acatagcagt agggacaatg ccttcattta acatttgcat tgtttgctga
     1261 aatcctgaca tagcagacca atctccttct ctttctgcta ttggttgtat ttgatttctt
     1321 gttaaatact tgtgccatcc tgctaatctc aatctggcac ttactgaact taagggacct
     1381 gcaagtagtg caatttgttg atggcctaat gcaacaaggt gttcaactcc taaccttgtt
     1441 ccatcttcgt gagaaaaaat tatagagttt attggggttt ggtccgacac atccaaaaac
     1501 aatgctggaa cattagtaca tgctgcttca actgcaatcg catcttggtc atcaagagga
     1561 taattaatta taagtccact aacacgttga gctaataaat tatgtactgc tgccttacaa
     1621 gcctctacac ctgatctctc aaccattgat acaacaacgc ttgcaccaag ttgatcagct
     1681 ctcgatttaa ttgcggctac aatttgacta ggcgcatgta atgcaagtga tgatgtggca
     1741 actccaatta aaagtgattg tttccccgca agttgctgag caacgcgatt tggtatataa
     1801 ttcaactctg ccatagctgc ttcaaccttc tctcttgttt ttgcagatac atgacttgct
     1861 tggttcacaa ctctcgaaac cgtttggtag ctaaccccgg catattcagc aacatcatac
     1921 aaagtaacag gtttcatgga tcattcctcc atgataaaat ttaaatttct gactttggca
     1981 aatccttttg cctgttaaaa agaatagcac aaaaattaaa aaaaaagaaa cgcaaatagc
     2041 aatagcatta aaaataatta ttattattta tttctttgat taaaatcaaa agcctttaat
     2101 gtattttctt gatctttcat gcgcttaacg gtaaaatcca aggtattaag ttcaccttcc
     2161 acttttctct ctctatcttc tctagaaaat cataaaaaat ttatttgctt tgtgagcgga
     2221 taacaattat actagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag
     2281 aggagaaatt acatatggat aagaaatact caataggctt agctatcggc acaaatagcg
     2341 tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg
     2401 gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg
     2461 gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga
     2521 agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata
     2581 gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc
     2641 atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct
     2701 atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt
     2761 tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc
     2821 ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat
     2881 ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat
     2941 tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg
     3001 gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt
     3061 ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag
     3121 ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt
     3181 tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc
     3241 ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa
     3301 aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa
     3361 aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta
     3421 tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg
     3481 aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact
     3541 tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca
     3601 atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg
     3661 cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat
     3721 ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga
     3781 caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg
     3841 agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa
     3901 aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa
     3961 atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg
     4021 atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg
     4081 atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct
     4141 tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac
     4201 ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt
     4261 atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg
     4321 gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc
     4381 tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac
     4441 aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag
     4501 gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc
     4561 cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa
     4621 attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc
     4681 ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc
     4741 tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt
     4801 atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg
     4861 tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag
     4921 tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta
     4981 agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt
     5041 ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg
     5101 atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga
     5161 ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac
     5221 gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg
     5281 ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt
     5341 atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat
     5401 atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag
     5461 agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata
     5521 aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca
     5581 agaaaacaga agtccagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt
     5641 cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata
     5701 gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga
     5761 agttaaaatc cgttaaagag ttactaggga tcacaattct ggaaagaagt tcctttgaaa
     5821 aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca
     5881 ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta
     5941 gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt
     6001 tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac
     6061 aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat
     6121 tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca
     6181 aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga
     6241 cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat
     6301 atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt
     6361 atgaaacacg cattgatttg agtcagctag gaggtgacta ataaaagctt gatcagatct
     6421 cagctttttt ttgaagtgcc tggcagtaag ttgaaaaaat ttgaaataaa cttggactat
     6481 gttaataaaa aaatacaaat taacgaacaa aaagatttga attattatat aatttagtta
     6541 aaagcaattt taaatgaggg aggtttccat atgagggaag cggtgatcgc cgaagtatcg
     6601 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     6661 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     6721 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     6781 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     6841 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     6901 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     6961 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     7021 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     7081 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     7141 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     7201 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     7261 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     7321 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgacgtcg ccggagcggt
     7381 cgagttctgg accgaccggc tcgggttctc atgaccaaaa tcccttaacg tgagttttcg
     7441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcactaga tccccggcaa
     8161 gttcatcctt agggttttca tatctcttaa aaagtcaatt tctgcttgat taatttcttg
     8221 tggattattg tttttgcggt ttttcatttt ttatctcctt gagtattgaa ttttttctta
     8281 tatataaata tatagcaaaa actatttttg ccaacttttt tacaaaaaat tttttattaa
     8341 aacacttagg gctttgctaa attctctttt aaaagaactt agtaaagccc tatggattta
     8401 agaactgatt atacttacgt agtaaaaata ctacagatat taatagtata gaccatatca
     8461 aaatcataat caatttatat ttatctaaaa ttgtagtcca tacaaaatac aaaaaaatta
     8521 ggtttttcta aattctattt taaaagaact tagtaacacc taatctctaa attacttaca
     8581 aaattctaaa attagatttt tagttcttca tatttcttta aaagtttttt aagaaaatct
     8641 ttttgatttt caaaaatttc atgcatcata aaactagtaa atttggattt ttttttgtaa
     8701 aaatcataac tttcttgggt tttaagttga aatcttaatg gttttattgg attttgccta
     8761 gattttttta acgccggact ttctttatct tttataaaat tataagaatt tttaatacca
     8821 ttttctatta aataatcttc ttttataagc ccctcttgca gtgcagttgc taatcttaaa
     8881 tacttatacg cttgagtttt tgctacatca aagtctttta taaaatcaag aaaagtttta
     8941 taaccatcaa atttataata ttgattatct tttatttctt ttaaaatttt aatattttcc
     9001 accctgtgga atatttcttt tttgaaattg tcatttaact tttcctttag tgtcttgtaa
     9061 taagaaattt tactttcatt aatcattaaa ttttgttcaa cataagtatc tatctttttt
     9121 gttaaaactt taacaacacg ttttttttcc ataaaaattt tcctttattt ttgcaacatt
     9181 ttccttaatt gatataattg ttactatttt agatttttta atatccactt aagaattaat
     9241 ccctcactag aatatttttt attatagaat tcatatacat gtttttctaa atctataaat
     9301 ttatcaacaa ttgctttatt atagggtgta tctatctttt cttttcttaa taatcgtctc
     9361 atattcttta aataacaaca aacactgccg gttttaaacc taaattctat gtaatatatt
     9421 tttgaaaaag tatatacatt ttcaaccccg tttatttcat actttacaaa aacattattt
     9481 attggttttc tataaccata gtaaatgcct aaaaatttgt ccccgtcttt cataggatat
     9541 agataaagtt tttgaatctc tgatttatct aataattttt caaaaaaaat tatacaccgc
     9601 ttttgtttgt tactaactac tctatgcaat gcctttaata tttttgtgtg atatatattt
     9661 ttatcacctt ttttttcttt cagcaggaag atactttctt ttggaaattt aattttttgt
     9721 tcatttattc ttgcagcctt aagtccgatt ttcatattta atccttatat agcctttcta
     9781 tttaattctt ttaaactcaa taaacttttt tggtttttca ttagttctaa taattcgcaa
     9841 taatatgtgc aaaacacttt attgtattct attttctttt ggttattcaa atattctttt
     9901 ataattggct ttagaagttc aatatttact tcttttttta gttgttcaat aaggatattg
     9961 aaaatgtttg tttttaaatc ttgataattt tgttgagaat tttctttttt tctttcaatt
    10021 gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact ttcaataata
    10081 aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc taattgttta
    10141 gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc ctttaatttt
    10201 tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt tagtaattta
    10261 atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc aatatttaaa
    10321 ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt acatttattg
    10381 aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc ttttatatta
    10441 tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa tctatcttta
    10501 aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa gtatttatag
    10561 attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc ggtccccaaa
    10621 tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc taatttgtaa
    10681 aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac tatttctttt aaggttttca
    10741 ttaaagtaat agagtatgtt tttttgtgtg tatttcttat atgttttatt tatgtacgct
    10801 agtgttgaga taaggactac taatttatac tgtaatttat tgtagc
//
