LOCUS       sequence-318342-       17422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 
            under 35S promoter/Plant transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 17422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD639
FEATURES             Location/Qualifiers
     source          1..17422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(199..570)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            603..712
                     /note="incP origin of transfer"
     primer_bind     935..954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1188..1205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1393..2043)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2508..2532
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2656..2835
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2688..2871
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2892..3686
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2946..2965)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3556..3575
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4076..4328
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4877..4898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4913..4918,4919..4936,4937..4943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4951..4967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4956..4978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4975..4991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4975..4991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5515..5860
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5882..5906
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7608..7632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7661..7750
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7784..8036
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8465..8717)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9185..10210)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9185..10210)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10220..10564)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10706..10723)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10706..10722)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10715..10737)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10862..10881)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11072..11093
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11340..11364
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12960..14108)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14407..15201)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15393..16160
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(16783..17422,1..72)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
       61 tcggaaaggc gctcggtctt gccttgctcg tcggtgatgt acttcaccag ctccgcgaag
      121 tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg
      181 ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt
      241 gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc
      301 gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc
      361 caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat
      421 tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc
      481 cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc
      541 cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
      601 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
      661 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
      721 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
      781 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
      841 ggaaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1201 gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt
     1261 ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt
     1321 ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg
     1381 cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga caacgagcct
     1441 ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc
     1501 ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac
     1561 ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca cggccccaac
     1621 agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc
     1681 gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc
     1741 ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt
     1801 cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg tatgattctc
     1861 cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta
     1921 aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac
     1981 gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct gcaactttgt
     2041 catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc agtgataaag
     2101 aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt gaaacccaac
     2161 atacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt
     2221 atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt caccgcccac
     2281 tatggcattc tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc
     2341 gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctcgctggc cggcgccaga
     2401 tctggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
     2461 gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa
     2521 tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatcatga
     2581 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta
     2641 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt
     2701 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
     2761 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc
     2821 ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc accggatctg
     2881 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     2941 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3001 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3061 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3121 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3181 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3241 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3301 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3361 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     3421 gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3481 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3541 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3601 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3661 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     3721 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3781 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3841 ggagttcttc gcccacggga tctctgcgga acaggcggtc gaaggtgccg atatcattac
     3901 gacagcaacg gccgacaagc acaacgccac gatcctgagc gacaatatga tcgggcccgg
     3961 cgtccacatc aacggcgtcg gcggcgactg cccaggcaag accgagatgc accgcgatat
     4021 cttgctgcgt tcggatattt tcgtggagtt cccgccacag acccggatga tccccgatcg
     4081 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     4141 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     4201 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     4261 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     4321 actagatcgg gcctcctgtc aatgctggcg gcggctctgg tggtggttct ggtggcggct
     4381 ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt
     4441 ccggtggtgg ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg
     4501 gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg
     4561 attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac gtttccggcc
     4621 ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag
     4681 tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta ccttccctcc
     4741 ctcaatcggt tgaatgtcgc ccttttgtct ttggcccaat acgcaaaccg cctctccccg
     4801 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4861 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4921 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4981 acagctatga ccatgattac gccaagcttg catgcctgca ggtccccaga ttagcctttt
     5041 caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat
     5101 caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa
     5161 agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag atatatttct
     5221 caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca
     5281 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
     5341 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     5401 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     5461 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     5521 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     5581 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     5641 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     5701 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     5761 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     5821 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc tagagttatc
     5881 aacaagtttg tacaaaaaag ctgccaccat gaagcaaagc tttgtggggg tttcttcaca
     5941 aacagaggct gagacaagag ccagagagag tgacacacag acacacccac aaaggaacct
     6001 cagtcactgt ccccaaaaac atcctcaaaa cttgcaaaga acccaaaatg gtgggtctgt
     6061 tgggggggca tcaatggcaa cagggatgat ggtgaggtca tcaccattca cagtgtctca
     6121 gtggcaggag ctggagcatc aagctttgat ctttaagtat ctaatggcag gactacccgt
     6181 cccacctgat cttgtgcttc ctattcagaa gagctttgaa tctatgtctt ctaggttctt
     6241 ccaccatcct accatgggtt actgttcctt ctatgggaag aaggtggacc cggagccagg
     6301 tagatgccgg aggactgatg gcaagaaatg gaggtgctcc aaagacgcct acccggactc
     6361 caagtactgt gagcgccaca tgcaccgtgg ccgcaaccgt tctagaaaac cggtcgaatc
     6421 acaaactact acacagtctt cttcgactgt gacttcacta actgtcactg gaggcagtgg
     6481 cagtggagcc gggaccttcc agaaccttcc cctgcaaaac ttcaataacc cccaaggcgg
     6541 cccggggtcc ggaaccaacc aatcccaatt ccatatggag cccattccct atggcattcc
     6601 taataaagat tacaggtatc ttcatggact taaacccgag gtaggcgaac atagtttctt
     6661 ctctgaagct tcaggaagca acaggggtct tcagatggac tcgcctttgg acagcacatg
     6721 gcctctgatg ccatcacggg tctcatcatt ccccccatca aaatcaagtg aaaactccat
     6781 cttgcagagt gactaccccc agcattcgtt cttcagcagc gaatttgcgt cgggagaggt
     6841 tgtgaaacag gagggtcagt ctctccgtcc tttctttgac gagtggccta aaaccagaga
     6901 ctcctggtcc ggccttgagg aagagaggtc caaccagacc tcattctcca caacccagct
     6961 ctcaatttcg atcccaatgg cctcctctga cttctcaaca accagctctc ggtctcccca
     7021 cgataacgcg gccgctgcca tgcagcccat gatggcagcc tattacccca gcaacgtcac
     7081 cactgatcac attcagcagt atcttgatga aaacaagtca ttgattctga agattgttga
     7141 gagccagaat tcaggaaaat tgactgaatg tgcagagaac caggcaagac tacagagaaa
     7201 cctcatgtac ctggctgcaa tcgctgattc tcaaccccaa ccacccacca tgcatgctca
     7261 gttccctcct agtggcattg ttcagccagg agctcactac atgcagcacc aacaagctca
     7321 acaaatgaca ccacagtcgc tcctggctgc acgctcctcc atgctgtact cccaacaacc
     7381 attttcggcc ctgcaacaac aacaagccat ccatagccag cttggcatgg gctctggtgg
     7441 aagtgcagga cttcacatgc tgcaaagcga ggggagtaat ccaggaggca atggaacact
     7501 ggggactgat atgggaaatg cagaagggcg aggagggaac tcaggaggtc agggtgggga
     7561 tggaggtgag actctttact tgaaagctgc tgaagatggg aattgacgtt cagctttctt
     7621 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     7681 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     7741 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     7801 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     7861 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     7921 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     7981 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     8041 attagcttca tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg
     8101 agcacagaaa ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt
     8161 tcggacgtgg gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc
     8221 ttggggggca gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga
     8281 tttatcttgg cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt
     8341 atgcctgtcg gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg
     8401 gcataccttt tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt
     8461 gatcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     8521 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     8581 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     8641 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     8701 caaatgtttg aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat
     8761 tgaaaacgtg ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc
     8821 tggatgattt gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg
     8881 catgcgtgag gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg
     8941 agcataattc ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt
     9001 tatgaggtga cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat
     9061 cattgcattc ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc
     9121 gttcgtggtc tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc
     9181 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     9241 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     9301 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     9361 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     9421 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     9481 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     9541 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     9601 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     9661 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     9721 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     9781 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     9841 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     9901 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     9961 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
    10021 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
    10081 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
    10141 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
    10201 gctttttcat atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg
    10261 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
    10321 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
    10381 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
    10441 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
    10501 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    10561 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    10621 gtcgtgctcc accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc
    10681 gtcgacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    10741 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    10801 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc
    10861 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    10921 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    10981 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    11041 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    11101 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca
    11161 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
    11221 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
    11281 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    11341 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    11401 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    11461 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    11521 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    11581 agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct
    11641 tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata
    11701 ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg
    11761 aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc
    11821 gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag
    11881 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
    11941 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
    12001 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
    12061 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
    12121 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
    12181 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
    12241 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
    12301 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
    12361 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
    12421 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
    12481 ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga
    12541 cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat
    12601 gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc
    12661 ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt
    12721 cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat
    12781 ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc
    12841 gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc
    12901 tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc
    12961 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
    13021 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
    13081 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
    13141 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
    13201 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
    13261 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
    13321 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
    13381 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
    13441 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
    13501 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
    13561 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
    13621 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
    13681 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
    13741 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
    13801 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
    13861 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
    13921 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
    13981 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
    14041 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
    14101 cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat
    14161 tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag
    14221 tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc
    14281 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
    14341 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
    14401 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    14461 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    14521 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    14581 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    14641 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    14701 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    14761 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    14821 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    14881 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    14941 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    15001 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    15061 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    15121 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    15181 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    15241 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    15301 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    15361 tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt
    15421 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
    15481 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc
    15541 tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt
    15601 acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc
    15661 attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg
    15721 taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt
    15781 ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc
    15841 ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta
    15901 tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg
    15961 aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc
    16021 ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct
    16081 caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc
    16141 aataagttgg agtcattacc caattatgat agaatttaca agctataagg ttattgtcct
    16201 gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga
    16261 taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat
    16321 aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc
    16381 tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta
    16441 aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg
    16501 ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc
    16561 ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga
    16621 agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg
    16681 atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc
    16741 ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga
    16801 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
    16861 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
    16921 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
    16981 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
    17041 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
    17101 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
    17161 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
    17221 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    17281 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    17341 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
    17401 ggcttcgacg gcgtttctgg cg
//
