LOCUS       sequence-318343-       17422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ 
            Plant transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 17422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD631
FEATURES             Location/Qualifiers
     source          1..17422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(259..630)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            663..772
                     /note="incP origin of transfer"
     primer_bind     995..1014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1248..1265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1453..2103)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2568..2592
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2716..2895
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2748..2931
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2952..3746
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3006..3025)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3616..3635
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4136..4388
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4937..4958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4973..4978,4979..4996,4997..5003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5011..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5016..5038
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5035..5051
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5035..5051
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5575..5920
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5942..5966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7668..7692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7721..7810
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7844..8096
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8525..8777)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9245..10270)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9245..10270)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10280..10624)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10766..10783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10766..10782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10775..10797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10922..10941)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11132..11153
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11400..11424
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13020..14168)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14467..15261)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15453..16220
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(16843..17422,1..132)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
       61 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
      121 tcggaaaggc gctcggtctt gccttgctcg tcggtgatgt acttcaccag ctccgcgaag
      181 tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg
      241 ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt
      301 gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc
      361 gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc
      421 caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat
      481 tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc
      541 cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc
      601 cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
      661 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
      721 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
      781 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
      841 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
      901 ggaaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1261 gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt
     1321 ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt
     1381 ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg
     1441 cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga caacgagcct
     1501 ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc
     1561 ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac
     1621 ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca cggccccaac
     1681 agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc
     1741 gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc
     1801 ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt
     1861 cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg tatgattctc
     1921 cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta
     1981 aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac
     2041 gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct gcaactttgt
     2101 catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc agtgataaag
     2161 aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt gaaacccaac
     2221 atacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt
     2281 atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt caccgcccac
     2341 tatggcattc tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc
     2401 gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctcgctggc cggcgccaga
     2461 tctggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
     2521 gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa
     2581 tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatcatga
     2641 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta
     2701 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt
     2761 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
     2821 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc
     2881 ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc accggatctg
     2941 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3001 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3061 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3121 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3181 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3241 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3301 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3361 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3421 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     3481 gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3541 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3601 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3661 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3721 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     3781 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3841 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3901 ggagttcttc gcccacggga tctctgcgga acaggcggtc gaaggtgccg atatcattac
     3961 gacagcaacg gccgacaagc acaacgccac gatcctgagc gacaatatga tcgggcccgg
     4021 cgtccacatc aacggcgtcg gcggcgactg cccaggcaag accgagatgc accgcgatat
     4081 cttgctgcgt tcggatattt tcgtggagtt cccgccacag acccggatga tccccgatcg
     4141 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     4201 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     4261 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     4321 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     4381 actagatcgg gcctcctgtc aatgctggcg gcggctctgg tggtggttct ggtggcggct
     4441 ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt
     4501 ccggtggtgg ctctggttcc ggtgattttg attatgaaaa gatggcaaac gctaataagg
     4561 gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg
     4621 attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac gtttccggcc
     4681 ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa atggctcaag
     4741 tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta ccttccctcc
     4801 ctcaatcggt tgaatgtcgc ccttttgtct ttggcccaat acgcaaaccg cctctccccg
     4861 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4921 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4981 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     5041 acagctatga ccatgattac gccaagcttg catgcctgca ggtccccaga ttagcctttt
     5101 caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat
     5161 caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa
     5221 agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag atatatttct
     5281 caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca
     5341 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
     5401 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
     5461 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     5521 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
     5581 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
     5641 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
     5701 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     5761 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     5821 atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     5881 ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc tagagttatc
     5941 aacaagtttg tacaaaaaag ctgccaccat gaagcaaagc tttgtggggg tttcttcaca
     6001 aacagaggct gagacaagag ccagagagag tgacacacag acacacccac aaaggaacct
     6061 cagtcactgt ccccaaaaac atcctcaaaa cttgcaaaga acccaaaatg gtgggtctgt
     6121 tgggggggca tcaatggcaa cagggatgat ggtgaggtca tcaccattca cagtgtctca
     6181 gtggcaggag ctggagcatc aagctttgat ctttaagtat ctaatggcag gactacccgt
     6241 cccacctgat cttgtgcttc ctattcagaa gagctttgaa tctatgtctt ctaggttctt
     6301 ccaccatcct accatgggtt actgttcctt ctatgggaag aaggtggacc cggagccagg
     6361 tagatgccgg aggactgatg gcaagaaatg gaggtgctcc aaagacgcct acccggactc
     6421 caagtactgt gagcgccaca tgcaccgtgg ccgcaaccgt tcaagaaagc ctgtggaatc
     6481 acaaactact acacagtctt cttcgactgt gacttcacta actgtcactg gaggcagtgg
     6541 cagtggagcc gggaccttcc agaaccttcc cctgcaaaac ttcaataacc cccaaggcgg
     6601 cccggggtcc ggaaccaacc aatcccaatt ccatatggag cccattccct atggcattcc
     6661 taataaagat tacaggtatc ttcatggact taaacccgag gtaggcgaac atagtttctt
     6721 ctctgaagct tcaggaagca acaggggtct tcagatggac tcgcctttgg acagcacatg
     6781 gcctctgatg ccatcacggg tctcatcatt ccccccatca aaatcaagtg aaaactccat
     6841 cttgcagagt gactaccccc agcattcgtt cttcagcagc gaatttgcgt cgggagaggt
     6901 tgtgaaacag gagggtcagt ctctccgtcc tttctttgac gagtggccta aaaccagaga
     6961 ctcctggtcc ggccttgagg aagagaggtc caaccagacc tcattctcca caacccagct
     7021 ctcaatttcg atcccaatgg cctcctctga cttctcaaca accagctctc ggtctcccca
     7081 cgataacgcg gccgctgcca tgcagcccat gatggcagcc tattacccca gcaacgtcac
     7141 cactgatcac attcagcagt atcttgatga aaacaagtca ttgattctga agattgttga
     7201 gagccagaat tcaggaaaat tgactgaatg tgcagagaac caggcaagac tacagagaaa
     7261 cctcatgtac ctggctgcaa tcgctgattc tcaaccccaa ccacccacca tgcatgctca
     7321 gttccctcct agtggcattg ttcagccagg agctcactac atgcagcacc aacaagctca
     7381 acaaatgaca ccacagtcgc tcctggctgc acgctcctcc atgctgtact cccaacaacc
     7441 attttcggcc ctgcaacaac aacaagccat ccatagccag cttggcatgg gctctggtgg
     7501 aagtgcagga cttcacatgc tgcaaagcga ggggagtaat ccaggaggca atggaacact
     7561 ggggactgat atgggaaatg cagaagggcg aggagggaac tcaggaggtc agggtgggga
     7621 tggaggtgag actctttact tgaaagctgc tgaagatggg aattgacgtt cagctttctt
     7681 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     7741 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     7801 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     7861 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     7921 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     7981 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     8041 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     8101 attagcttca tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg
     8161 agcacagaaa ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt
     8221 tcggacgtgg gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc
     8281 ttggggggca gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga
     8341 tttatcttgg cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt
     8401 atgcctgtcg gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg
     8461 gcataccttt tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt
     8521 gatcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     8581 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     8641 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     8701 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     8761 caaatgtttg aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat
     8821 tgaaaacgtg ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc
     8881 tggatgattt gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg
     8941 catgcgtgag gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg
     9001 agcataattc ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt
     9061 tatgaggtga cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat
     9121 cattgcattc ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc
     9181 gttcgtggtc tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc
     9241 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     9301 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     9361 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     9421 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     9481 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     9541 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     9601 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     9661 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     9721 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     9781 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     9841 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     9901 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     9961 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
    10021 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
    10081 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
    10141 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
    10201 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
    10261 gctttttcat atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg
    10321 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
    10381 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
    10441 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
    10501 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
    10561 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    10621 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    10681 gtcgtgctcc accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc
    10741 gtcgacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    10801 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    10861 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc
    10921 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    10981 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    11041 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    11101 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    11161 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca
    11221 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
    11281 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
    11341 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    11401 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    11461 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    11521 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    11581 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    11641 agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct
    11701 tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata
    11761 ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg
    11821 aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc
    11881 gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag
    11941 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
    12001 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
    12061 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
    12121 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
    12181 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
    12241 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
    12301 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
    12361 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
    12421 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
    12481 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
    12541 ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga
    12601 cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat
    12661 gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc
    12721 ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt
    12781 cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat
    12841 ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc
    12901 gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc
    12961 tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc
    13021 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
    13081 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
    13141 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
    13201 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
    13261 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
    13321 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
    13381 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
    13441 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
    13501 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
    13561 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
    13621 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
    13681 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
    13741 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
    13801 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
    13861 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
    13921 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
    13981 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
    14041 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
    14101 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
    14161 cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat
    14221 tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag
    14281 tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc
    14341 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
    14401 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
    14461 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    14521 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    14581 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    14641 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    14701 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    14761 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    14821 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    14881 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    14941 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    15001 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    15061 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    15121 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    15181 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    15241 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    15301 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    15361 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    15421 tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt
    15481 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
    15541 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc
    15601 tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt
    15661 acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc
    15721 attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg
    15781 taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt
    15841 ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc
    15901 ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta
    15961 tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg
    16021 aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc
    16081 ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct
    16141 caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc
    16201 aataagttgg agtcattacc caattatgat agaatttaca agctataagg ttattgtcct
    16261 gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga
    16321 taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat
    16381 aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc
    16441 tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta
    16501 aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg
    16561 ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc
    16621 ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga
    16681 agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg
    16741 atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc
    16801 ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga
    16861 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
    16921 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
    16981 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
    17041 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
    17101 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
    17161 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
    17221 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
    17281 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    17341 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    17401 gtgagggcca agttttccgc ga
//
