LOCUS       sequence-318344-       17395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ 
            Plant transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17395)
  AUTHORS   Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik 
            JF, Dubcovsky J
  TITLE     A GRF-GIF chimeric protein improves the regeneration efficiency of 
            transgenic plants.
  JOURNAL   Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0.
  PUBMED    33046875
REFERENCE   2  (bases 1 to 17395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 
            10.1038/s41587-020-0703-0."
COMMENT     Sequence Label: JD690
FEATURES             Location/Qualifiers
     source          1..17395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(107..478)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            511..620
                     /note="incP origin of transfer"
     primer_bind     843..862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1096..1113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1301..1951)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2416..2440
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2564..2743
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2596..2779
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2800..3594
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2854..2873)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3464..3483
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      3984..4236
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4785..4806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4821..4826,4827..4844,4845..4851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4859..4875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4864..4886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4883..4899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4883..4899
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5423..5768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5790..5814
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7489..7513)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7542..7631
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7665..7917
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8346..8598)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9066..10091)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9066..10091)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10101..10445)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10587..10604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10587..10603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10596..10618)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10743..10762)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10953..10974
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11221..11245
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12841..13989)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14288..15082)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15274..16041
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16664..17375
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca tggggacgtg
       61 cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat ggctctgccc
      121 tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct tcgatcttcg
      181 ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc
      241 agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc agctcgcgga
      301 cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc agcaggtagg
      361 ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa
      421 ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc
      481 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
      541 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
      601 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
      661 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
      721 taatgacccc gaagcagggt tatgcagcgg aaaagcgcca cgcttcccga agggagaaag
      781 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      841 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      901 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      961 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1021 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1081 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcca gaaggccgcc
     1141 agagaggccg agcgcggccg tgaggcttgg acgctagggc agggcatgaa aaagcccgta
     1201 gcgggctgct acgggcgtct gacgcggtgg aaagggggag gggatgttgt ctacatggct
     1261 ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca ccctttctcg
     1321 gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca atcaccgcga
     1381 gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca
     1441 acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc
     1501 cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc agcgcattga
     1561 cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt
     1621 ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga
     1681 gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg
     1741 ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt gcgtcgagca
     1801 gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg
     1861 ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc agggcggcac
     1921 ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg ataaacataa
     1981 tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc agcggaggct
     2041 ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga gcactgtcgc
     2101 gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc tgcgcgatct
     2161 ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt atgcgttggt
     2221 gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat
     2281 cttgctcgtc tcgctggccg gcgccagatc tggggaaccc tgtggttggc atgcacatac
     2341 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     2401 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     2461 aaggcgggaa acgacaatct gatcatgagc ggagaattaa gggagtcacg ttatgacccc
     2521 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
     2581 gagccactca gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     2641 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
     2701 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     2761 tcaatccaaa taatctgcac cggatctgga tcgtttcgca tgattgaaca agatggattg
     2821 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     2881 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     2941 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     3001 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3061 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3121 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     3181 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     3241 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     3301 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc
     3361 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     3421 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     3481 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     3541 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     3601 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     3661 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     3721 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccacgggatc tctgcggaac
     3781 aggcggtcga aggtgccgat atcattacga cagcaacggc cgacaagcac aacgccacga
     3841 tcctgagcga caatatgatc gggcccggcg tccacatcaa cggcgtcggc ggcgactgcc
     3901 caggcaagac cgagatgcac cgcgatatct tgctgcgttc ggatattttc gtggagttcc
     3961 cgccacagac ccggatgatc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     4021 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     4081 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     4141 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     4201 aattatcgcg cgcggtgtca tctatgttac tagatcgggc ctcctgtcaa tgctggcggc
     4261 ggctctggtg gtggttctgg tggcggctct gagggtggtg gctctgaggg tggcggttct
     4321 gagggtggcg gctctgaggg aggcggttcc ggtggtggct ctggttccgg tgattttgat
     4381 tatgaaaaga tggcaaacgc taataagggg gctatgaccg aaaatgccga tgaaaacgcg
     4441 ctacagtctg acgctaaagg caaacttgat tctgtcgcta ctgattacgg tgctgctatc
     4501 gatggtttca ttggtgacgt ttccggcctt gctaatggta atggtgctac tggtgatttt
     4561 gctggctcta attcccaaat ggctcaagtc ggtgacggtg ataattcacc tttaatgaat
     4621 aatttccgtc aatatttacc ttccctccct caatcggttg aatgtcgccc ttttgtcttt
     4681 ggcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4741 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4801 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4861 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
     4921 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
     4981 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
     5041 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
     5101 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
     5161 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
     5221 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
     5281 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
     5341 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
     5401 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
     5461 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
     5521 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
     5581 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     5641 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     5701 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     5761 agagaacacg ggggactcta gagttatcaa caagtttgta caaaaaagct gccaccatga
     5821 atagcggcaa cggcggtggc ggtgctggtg gggctggtgg gttggggatg atgggaatga
     5881 gggcatcacc ttttacggta tcacaatggc aagaactgga acatcaagct ttgatcttta
     5941 agtacatggt ggcaggtttg cctgtgccac ctgatcttgt actccctata caaaagagct
     6001 ttgagtctat ttctcatagg ttctttcatc accccaccat gggttattgt tccttctatg
     6061 ggaaaaaagt tgatcccgaa ccaggacggt gcaggaggac tgacggcaaa aaatggcggt
     6121 gttccaaaga tgcataccca gactcaaagt actgtgagcg ccacatgcac cgcggccgca
     6181 accgttcaag aaagcctgtg gaatcacaaa ccataacaca gtcatcatcc actgtgacat
     6241 cactgactgc cactggaagc agtggtggga ctggaagcta ccagaacctt ccattacatg
     6301 cctttgctaa tccccaaggc actacttctg gaaccaacca atcccattat catgtggact
     6361 ccatttcctg tggaattccc aacaaagaat acaggtacct tcaagttcaa ggacttaaac
     6421 ccgaggttgg agagcatagt ttcttcactg aagcttcagg aagcaatagg ggtctccaga
     6481 tggactcacc aattgacagt gcatggcctc tgatgcaatc tagagtctcc tcgcatcccc
     6541 agtcgaaatc cagcaacacc tccattttgc agaatgatta tcctcagcat tcacttttca
     6601 gtagtgaatt cacctcgggt gagcctgtaa aacaggaagg tcagtctctc cgacctttct
     6661 ttgatgagtg gcctaagaca agagactcat ggtctggtct tgaagacaag agatcccacc
     6721 agacctcatt ctcaacaacc aagctctcta tatccatccc tatggcctca tccgacttct
     6781 cggcaacgag ttctcgatcg cctcatgata acgcggccgc tgccatgcaa cagcacctga
     6841 tgcagatgca gcccatgatg gcagcttatt atcccaacaa cgtcactact gaccacattc
     6901 aacagtatct agatgagaac aaatcattga ttttgaagat tgttgagagc cagaattcag
     6961 ggaaactgag cgagtgtgca gagaaccagg caagattgca gcggaatctc atgtacctgg
     7021 ctgctattgc tgatgctcaa ccccaaccac ctagcgttca tgcccagttc tcttctggtg
     7081 gcattatgca gccaggagct cactatatgc aacaccagca atctcagcca atgacaccac
     7141 agtcacttat ggctgcacgc tcatccatgg tgtactctca acagcaattt tcagtgcttc
     7201 agcaacagca agccttgcat ggtcagcttg gcatgagctc tggtggtagc tcaggacttc
     7261 acatgctgca aagtgagggt agtactgcag gaggtagtgg ttcacttggg ggtgggggat
     7321 tccctgattt tggccgtggc tcatctggtg aaggcttgca ctcaagggga atggggagca
     7381 agcatgatat aggcagttct ggatctgctg aaggacgagg agggagctca ggaagccaag
     7441 atggaggcga aactctctac ttgaaagggg ctgatgatgg aaattaacgt tcagctttct
     7501 tgtacaaagt ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg
     7561 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     7621 cagattacgc tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt
     7681 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     7741 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     7801 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     7861 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     7921 aattagcttc atcaacgcaa gacatgcgca cgaccgtctg acaggagagg aatttccgac
     7981 gagcacagaa aggacttgct cttggacgta ggcctatttc tcaggcacat gtatcaagtg
     8041 ttcggacgtg ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt
     8101 cttggggggc agtcagcagt tcatttcaca agacagagga acttgtaagg agatgcactg
     8161 atttatcttg gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga atatctaagt
     8221 tatgcctgtc ggcatgagca gaaacttcca attcgaaaca gtttggagag gttgtttttg
     8281 ggcatacctt ttgttagtca gcctctcgat tgctcatcgt cattacacag taccgaagtt
     8341 tgatcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     8401 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     8461 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     8521 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     8581 ccaaatgttt gaacgatctg cttcgacgca ctccttcttt actccaccat ctcgtcctta
     8641 ttgaaaacgt gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt accaatcacg
     8701 ctggatgatt tgccagttgg attaatcttg cctttccccg catgaataat attgatgaat
     8761 gcatgcgtga ggggtatttc gattttggca atagctgcaa ttgccgcgac atcctccaac
     8821 gagcataatt cttcagaaaa atagcgatgt tccatgttgt cagggcatgc atgatgcacg
     8881 ttatgaggtg acggtgctag gcagtattcc ctcaaagttt catagtcagt atcatattca
     8941 tcattgcatt cctgcaagag agaattgaga cgcaatccac acgctgcggc aaccttccgg
     9001 cgttcgtggt ctatttgctc ttggacgttg caaacgtaag tgttggatcc cggtcggcat
     9061 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     9121 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     9181 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     9241 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     9301 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     9361 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     9421 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     9481 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     9541 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     9601 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     9661 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     9721 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     9781 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     9841 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     9901 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     9961 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
    10021 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
    10081 ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat atagaggaag
    10141 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    10201 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    10261 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    10321 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    10381 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    10441 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    10501 tgtcgtgctc caccatgttg acggatctct aggacgcgtc gacgcgtcga cgcgtcgacg
    10561 cgtcgacgcg tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
    10621 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10681 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc
    10741 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    10801 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    10861 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    10921 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10981 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggaacc
    11041 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
    11101 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
    11161 accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    11221 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    11281 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    11341 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    11401 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    11461 cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc
    11521 ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat
    11581 aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt
    11641 gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc
    11701 cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga
    11761 gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga
    11821 agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg
    11881 taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg
    11941 ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca
    12001 gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat
    12061 ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc
    12121 ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca
    12181 tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa
    12241 taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc
    12301 accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg
    12361 cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg
    12421 acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa
    12481 tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg
    12541 cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct
    12601 tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa
    12661 tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg
    12721 cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc
    12781 ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
    12841 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
    12901 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
    12961 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
    13021 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
    13081 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
    13141 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
    13201 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
    13261 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
    13321 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
    13381 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
    13441 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
    13501 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
    13561 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
    13621 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
    13681 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
    13741 cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
    13801 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
    13861 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
    13921 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
    13981 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
    14041 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
    14101 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
    14161 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
    14221 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
    14281 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
    14341 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
    14401 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
    14461 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
    14521 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
    14581 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
    14641 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
    14701 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
    14761 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
    14821 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
    14881 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
    14941 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    15001 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    15061 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
    15121 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
    15181 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
    15241 atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag
    15301 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
    15361 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct
    15421 ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg
    15481 tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg
    15541 cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg
    15601 gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag
    15661 tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc
    15721 cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt
    15781 atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat
    15841 gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag
    15901 cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc
    15961 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
    16021 caataagttg gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc
    16081 tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg
    16141 ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata
    16201 taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc
    16261 ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt
    16321 aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc
    16381 gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg
    16441 cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg
    16501 aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc
    16561 gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat
    16621 cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg
    16681 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc
    16741 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc
    16801 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg
    16861 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga
    16921 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt
    16981 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc
    17041 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc
    17101 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc
    17161 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc
    17221 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca
    17281 cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc
    17341 gagggcaacc agcccggtga gcgtcggaaa ggcgctcggt cttgccttgc tcgtc
//
