LOCUS       sequence-318380-        9916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of AID-BE4max-P2A-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu
            H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang 
            Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D
  TITLE     Dual base editor catalyzes both cytosine and adenine base 
            conversions in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y.
  PUBMED    32483363
REFERENCE   2  (bases 1 to 9916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y."
COMMENT     Sequence Label: AID-BE4max-P2A-GFP
FEATURES             Location/Qualifiers
     source          1..9916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(12..600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(92..111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(771..1562,1563..1631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1394..1413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1632..1736)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1815..1834)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2026..2274
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2026..2046
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2197..2216
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2293..2368
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        2388..2767
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2768..2971
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2922..2942
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3053..3073
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3665..3688,3689..3691,3692..7765)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3665..7765
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7796..8044
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8075..8323
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8366..8386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8396..8452
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8453..8455,8456..8458,8459..9169)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8453..8455,8456..8458,8459..9169)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8759..8778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9106..9127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9182..9202
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9225..9242)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9231..9455
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9539..9561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9550..9566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9574..9580,9581..9598,9599..9604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9619..9640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9757..9774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
       61 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      421 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      901 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1741 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1801 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1861 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1921 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1981 gatgtacggg ccagatatac gcgtggatcc ctgcagttaa ttaaagaggg cctatttccc
     2041 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     2101 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     2161 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     2221 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct
     2281 tcgagaagac ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     2341 acttgaaaaa gtggcaccga gtcggtgctt tttttatgca tggtaccgac attgattatt
     2401 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2941 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagag
     3001 atccgcggcc gccaccatga agaggaccgc cgatggctct gagttcgaga gccccaagaa
     3061 gaagcggaag gtggacagcc tgctgatgaa ccggagaaag ttcctgtatc agtttaagaa
     3121 tgtgaggtgg gcaaagggca ggcgcgagac ctacctgtgc tatgtggtga agcggagaga
     3181 tagcgccaca tctttcagcc tggactttgg ctacctgcgg aacaagaatg gctgccacgt
     3241 ggagctgctg tttctgagat acatctccga ctgggatctg gacccaggca ggtgttatcg
     3301 cgtgacctgg ttcacatcct ggtctccctg ctacgattgt gcccggcacg tggccgactt
     3361 tctgagaggc aaccctaatc tgtctctgag gatcttcacc gcccgcctgt atttttgcga
     3421 ggataggaag gccgagccag agggactgag gcgcctgcac agggccggcg tgcagatcgc
     3481 catcatgaca ttcaaggact acttttattg ttggaacacc ttcgtggaga atcacgagcg
     3541 gacatttaag gcctgggagg gactgcacga gaactccgtg cggctgtcta gacagctgcg
     3601 gagaatcctg ctgccaagcg gctccgagac cccaggcaca tctgagagcg ccacccctga
     3661 gagcgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
     3721 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     3781 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     3841 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     3901 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     3961 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     4021 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     4081 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     4141 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     4201 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     4261 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     4321 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     4381 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     4441 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     4501 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     4561 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     4621 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     4681 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     4741 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     4801 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     4861 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     4921 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     4981 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     5041 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     5101 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     5161 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     5221 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     5281 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     5341 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     5401 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     5461 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     5521 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     5581 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     5641 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     5701 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     5761 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     5821 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     5881 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     5941 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     6001 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     6061 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     6121 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca
     6181 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     6241 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     6301 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     6361 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     6421 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     6481 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     6541 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     6601 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     6661 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     6721 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     6781 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     6841 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     6901 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     6961 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     7021 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     7081 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     7141 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     7201 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     7261 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     7321 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     7381 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     7441 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     7501 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     7561 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     7621 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     7681 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     7741 cgacctgtct cagctgggag gtgacagcgg cgggagcggc gggagcgggg ggagcactaa
     7801 tctgagcgac atcattgaga aggagactgg gaaacagctg gtcattcagg agtccatcct
     7861 gatgctgcct gaggaggtgg aggaagtgat cggcaacaag ccagagtctg acatcctggt
     7921 gcacaccgcc tacgacgagt ccacagatga gaatgtgatg ctgctgacct ctgacgcccc
     7981 cgagtataag ccttgggccc tggtcatcca ggattctaac ggcgagaata agatcaagat
     8041 gctgagcgga ggatccggag gatctggagg cagcaccaac ctgtctgaca tcatcgagaa
     8101 ggagacaggc aagcagctgg tcatccagga gagcatcctg atgctgcccg aagaagtcga
     8161 agaagtgatc ggaaacaagc ctgagagcga tatcctggtc cataccgcct acgacgagag
     8221 taccgacgaa aatgtgatgc tgctgacatc cgacgcccca gagtataagc cctgggctct
     8281 ggtcatccag gattccaacg gagagaacaa aatcaaaatg ctgtctggcg gctcaaaaag
     8341 aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg aaagtcggaa gcggagctac
     8401 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctatggtgag
     8461 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     8521 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     8581 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     8641 caccctgacc tatggagtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     8701 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     8761 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     8821 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     8881 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     8941 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     9001 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     9061 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     9121 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ctggtggttc
     9181 tcccaagaag aaaaggaaag tctaagttta aacccgctga tcagcctcga ctgtgccttc
     9241 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     9301 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     9361 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa
     9421 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     9481 gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agatgtttcc
     9541 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9601 taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9661 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9721 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9901 ccgtaaaaag gccgcg
//
