LOCUS       sequence-318400-        9904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 9904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-MGT#2-mCherry
FEATURES             Location/Qualifiers
     source          1..9904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1052..1070
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1088..1106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1156..1863
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1298..1316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1566..1585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1993..2581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2046..2066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2464..2475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2584..2600)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2585..2601)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3111..3291
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3317..3334)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3323..3547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3593..4021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3680..3699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3890..3911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4030..4050)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4035..4364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4215..4350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4277..4296
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4412..4459
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4478..4852
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4982..5103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5019..5038)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5073..5092
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5152..5168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5152..5168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5165..5187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5176..5192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5200..5206,5207..5224,5225..5230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5245..5266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5383..5400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5554..6142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5634..5653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6313..7104,7105..7173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6936..6955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7174..7278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7353..7372)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7544..7923
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7925..8123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8078..8098
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8141..8321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8368..8493
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8986..9219
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9404..9448
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9746..9863
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taatgcatct agaacagcag ggccacctcc ttctctcccc cgcgggcatg ggcccccacc
       61 cccactgccg gcagagtgct gaggactcgt gcaccatgag aacttctgac catgagaact
      121 ttgacttccg gatttggggg atctgcccag gtgaacacaa tgcaaggggc tgcatgacct
      181 accaggacag aactttcccc aattacaggg tgactcacag ccgcattggt gactcacttc
      241 aatgtgtcat ttccggctgc tgtgtgtgag cagtggacac gtgagggggg ggtgggtgag
      301 agagacaggc cacattgtgc aacagatctc tagagctttt tcatcttgca aaactgaaac
      361 tgtataccca tggaacaaca gctccctgct cccctccccc tcagctcctg ggtagtgaca
      421 tttcttgatt ctcagtaaac tatcacaaga acaaaaaacc aaacaccgca tattctcact
      481 cataggtggg aattgaacaa tgagatcaca tggacacagg aaggggaata tcacactctg
      541 gggactgtgg tggggtgggg ggagtgggga gggatagcac tgggatgtcc caagagaagg
      601 ggaagagggg gaggtgttag agaacttgtg tgttcaaccg aaacatgatg aaaacaggga
      661 aagcccccaa gatacctgtc attcccgatg atgtcagatt cagcaaattc aatgataaca
      721 aaacattatg aaaaaattag taattaaaat aatacagcaa tgtgtatgaa caaaataatc
      781 aatgaaagtg aaacctaata gtaattccac aaacttatta caaagctatt aatttaaaga
      841 gtagtggcaa ttgaaaacca caaccaacac cagtgcttac agcagcaata cttttactca
      901 gacttcctgt ttctggaact tgccttcttt tttgctgtgt ttatacttcc cttgtctgtg
      961 gttagataag tataaagccc tagatctaag cttctctgtc ttcctccctc cctcccttcc
     1021 tcttactctc attcattgta cactagttta ctccctatca gtgatagaga acgtatatcc
     1081 agtttactcc ctatcagtga tagagaattc atgtatggca ggaagaagcg gagacagcga
     1141 cgaagaaagc ttggggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     1201 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     1261 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     1321 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     1381 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     1441 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     1501 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1561 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1621 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1681 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1741 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1801 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctatacaag
     1861 taaaccggta tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     1921 tgctttaaaa aacctcccac acgtacaagt aaagcggccg cgactctaca attcgatatc
     1981 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2041 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2101 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2161 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2221 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2281 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2341 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2401 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2461 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2521 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2581 catcgatacc gtcgacctcg atcgaggacc tagaaaaaca tggagcaatc acaagtagca
     2641 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     2701 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     2761 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     2821 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     2881 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     2941 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     3001 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     3061 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     3121 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3181 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3241 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     3301 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3361 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3421 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3481 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3541 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3901 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3961 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4021 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4081 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4201 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4261 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4321 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4381 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     4441 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     4501 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     4561 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     4621 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     4681 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     4741 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     4801 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     4861 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4921 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4981 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5041 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5101 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5161 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5221 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5341 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6301 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6361 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6421 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6601 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7141 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7261 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7321 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7381 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     7441 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     7501 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     7561 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7621 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7681 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7741 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7801 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7861 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7921 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     7981 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     8041 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     8101 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     8161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8341 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8401 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8461 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8521 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8581 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8641 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8701 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8761 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8821 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8881 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8941 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9001 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9061 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9121 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9181 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9241 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9301 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9361 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9421 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9481 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9541 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9601 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9661 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     9721 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9781 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9841 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     9901 taat
//
