LOCUS       sequence-318402-        9921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of proneural Glioblastoma 
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-PNGT#1-mCherry
FEATURES             Location/Qualifiers
     source          1..9921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             119..826
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(261..279)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     529..548
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    956..1544
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1009..1029)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1427..1438)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1547..1563)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1548..1564)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2074..2254
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2280..2297)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2286..2510
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2556..2984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2643..2662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2853..2874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2993..3013)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2998..3327
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3178..3313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3240..3259
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3375..3422
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3441..3815
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3945..4066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3982..4001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4036..4055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4115..4131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4115..4131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4128..4150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4139..4155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4163..4169,4170..4187,4188..4193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4208..4229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4346..4363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4517..5105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4597..4616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5276..6067,6068..6136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5899..5918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6137..6241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6316..6335)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6507..6886
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6888..7086
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7041..7061
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7104..7284
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7331..7456
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7949..8182
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8367..8411
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8709..8826
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtacactagt ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag
       61 tgatagagaa ttcatgtatg gcaggaagaa gcggagacag cgacgaagaa agcttggggt
      121 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      181 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      241 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      301 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      361 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      421 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      481 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
      541 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
      601 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
      661 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
      721 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
      781 cgagggccgc cactccaccg gcggcatgga cgagctatac aagtaaaccg gtatctagat
      841 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      901 cacacgtaca agtaaagcgg ccgcgactct acaattcgat atcaagctta tcgataatca
      961 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1021 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1081 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1141 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1201 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1261 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1321 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     1381 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     1441 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     1501 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     1561 tcgatcgagg acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     1621 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     1681 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     1741 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     1801 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     1861 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     1921 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     1981 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     2041 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     2101 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2161 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2221 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     2281 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     2341 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     2401 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     2461 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     2521 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     2581 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2641 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2701 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2761 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2821 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2881 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2941 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     3001 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3061 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3121 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3181 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3241 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     3301 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     3361 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     3421 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     3481 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     3541 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     3601 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     3661 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     3721 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     3781 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     3841 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     3901 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     3961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     4081 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6241 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     6301 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     6361 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     6421 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     6481 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     6541 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6601 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6661 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6721 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6781 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6841 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6901 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6961 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     7021 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     7081 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7141 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7201 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7261 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7321 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7381 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7441 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7501 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7561 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7621 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7681 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7741 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7801 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7861 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7921 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7981 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8041 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8101 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     8161 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8221 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8281 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8341 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8401 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8461 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8521 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8581 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     8641 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     8701 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8761 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8821 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa agagctcctg
     8881 gccaaggtct ttgtgttcag accagaagag gaaggagggc tccctccccc tggggctgtg
     8941 gaggctgagg ctcctggggg gttgtccaca tctggaccgt gggagctgtt ggggggaatg
     9001 ggggcaggtg gagaagagga taagcagctg attgggccca gactactctg ggctggctcc
     9061 atcttacatg actgccacaa acagctgcag gagtgtgaca gatcacaaca ctagcattgt
     9121 acctcaaaat atgcttgtac cctaaggcac aagaactggt ttgacttaca accgcagccc
     9181 ccgtccgggc accccgaggc ccgcgggagc caccctcgaa ccccggccgc gcacgggcgg
     9241 ggcggcgcgc acctgccggg agcccgtgtt tgtaaacaaa ccgcgcgcct aattagcctg
     9301 gcgggagcgc gcgcgcgggg cggggggcgg ggcgtcggtg cgcgcgggca ggtcggcccc
     9361 gcccggggag gagccgcgct ctgccgcgcc ctccgtgtca ccatctcccc cacccgactt
     9421 ggcggggcgc gggcttgctg gagcctgcgg gacccagagc ccgctccgga gccagccctg
     9481 ggagtggcca gcttgaaccc gagggccccg cagaccgtta ctccggcccc cgcccggggc
     9541 ggggcgcgcg ggggcgcggc gcagcccaac ccgcacagcc gcgtccccaa acaccaccga
     9601 ggagggaaaa cagacggaga ggggtggggc tgcgggcggg gccggcgcct aattgggccg
     9661 cgggcgcctc gaggtgggcg gggcataagg gggcggggcc gcggagaccc cgggcgggag
     9721 cagggagagg aaagaagaga ctgagtacgc ggagaccgag attcggaaat atttctgcct
     9781 taattgttct tccattgtct ttctcctgtg ggtcccctct cacctttctg tatggtcctg
     9841 gatcaccccc cgaggctttg tctcccccat ccacgggctt attctctcgg cacccccttc
     9901 ctctcccgtc atcggttgat t
//
