LOCUS       sequence-318404-       10021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of proneural Glioblastoma 
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-PNGT#2-mCherry
FEATURES             Location/Qualifiers
     source          1..10021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1169..1187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1205..1223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1273..1980
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1415..1433)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1683..1702
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2110..2698
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2163..2183)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2581..2592)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2701..2717)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2702..2718)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3228..3408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3434..3451)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3440..3664
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3710..4138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3797..3816)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4007..4028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4147..4167)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4152..4481
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4332..4467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4394..4413
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4529..4576
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4595..4969
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5099..5220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5136..5155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5190..5209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5269..5285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5269..5285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5282..5304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5293..5309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5317..5323,5324..5341,5342..5347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5362..5383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5500..5517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5671..6259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5751..5770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6430..7221,7222..7290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7053..7072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7291..7395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7470..7489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7661..8040
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8042..8240
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8195..8215
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8258..8438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8485..8610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9103..9336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9521..9565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9863..9980
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taatccctcc tctaatccct ccagcgggat cagggaggag gtgcgggacc tgctgccccg
       61 ggcttgcccc catcccggcc tcacgcatgg gcgcctgtct cagccctctc ccaggacgct
      121 gcaggtgtgg ctgggccagc gctaattagt gggccgcgcg ggggccccgc tgagcctttg
      181 acagaaaagg cggtagggag gtgggggcag ggaggcgctc caccagccag aagtccggag
      241 cgcaacccaa agtactccat ctcaaaagaa aaaaggcggg ggcggtgggg ggggggggtg
      301 atttcagtac aaagcctaca gacattataa aaatattaag atttttgttc gtttgttttt
      361 tgtttttgag acagagtctc actgtcaccc ccaggctgga gtctgtgccg gcgcccgctg
      421 cttcgcatct gcgcgcccgc ccggtgccgg gccccgccct ccgcctcagc cccaagctcg
      481 gcccgcgggc ccggccacag gtgccccggc ggccccgcct ggcccgaggg aagagggcag
      541 ctgggagggg cccatgagag aaccaaaact gtgcccccag gcttggaaag aaatcacatg
      601 tatggccagc aggaaggttc cggaaggttc cggaggacac ctgcaggtgg gactgagaac
      661 aggggtctcg gctgggagtg gctgaggcca tatgaggacc tcgactgcca caaacagctg
      721 caggagtgtg acagatcaca acactagcat tgtacctcaa aatatgcttg taccctaagg
      781 cacaagaact ggtttgactt acaaaactga tctcagagtt gggatcaaag tttttctacc
      841 actctactat gagccctcgg ccgggccccg ccccgccagc tccgcgcggc tctgggctct
      901 ctaggggtgg ggctgcgggc ggggccggcg cctaattggg ccgcgggcgc ctcgaggtgg
      961 gcggggcata agggggcggg gccgcggaga ccccgggcgg gagttttttt ctgcaagcga
     1021 gagggggggt gttgttggta tcgccccctc cttctcctcc ccccaggggt gaaagtgcaa
     1081 gaggaagtgc agccgctgcc atctttcctc cgctccgaac acacggagcc cggggccgca
     1141 cagccgccgc tcctgtacac tagtttactc cctatcagtg atagagaacg tatatccagt
     1201 ttactcccta tcagtgatag agaattcatg tatggcagga agaagcggag acagcgacga
     1261 agaaagcttg gggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1321 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1381 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1441 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1501 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1561 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1621 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1681 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1741 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1801 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1861 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1921 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct atacaagtaa
     1981 accggtatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
     2041 tttaaaaaac ctcccacacg tacaagtaaa gcggccgcga ctctacaatt cgatatcaag
     2101 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2161 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2221 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2281 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2341 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2401 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2461 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     2521 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2581 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     2641 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     2701 cgataccgtc gacctcgatc gaggacctag aaaaacatgg agcaatcaca agtagcaata
     2761 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     2821 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     2881 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     2941 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     3001 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     3061 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     3121 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     3181 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     3241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     3421 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3481 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3541 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3601 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3661 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     3721 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3781 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3841 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3901 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3961 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4021 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4081 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4141 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4201 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4261 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4321 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4381 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4441 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4501 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     4561 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     4621 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     4681 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     4741 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     4801 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     4861 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     4921 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     4981 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5041 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5221 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5281 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5341 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5401 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5461 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5521 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5581 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5641 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5701 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5761 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5821 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5881 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5941 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6001 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6061 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6121 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6181 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6241 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6301 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6361 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6421 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6481 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6541 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6601 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6661 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6721 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6781 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6841 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6901 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6961 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7021 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7081 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7141 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7201 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7261 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7321 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7381 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7441 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7501 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     7561 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     7621 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     7681 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     7741 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     7801 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     7861 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     7921 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     7981 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8041 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8101 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8161 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8221 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     8281 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8341 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8401 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8461 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8521 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8581 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8641 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8701 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8761 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8821 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8881 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8941 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9001 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9061 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9121 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9181 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9241 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9301 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9361 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9421 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9481 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9541 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9601 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9661 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9721 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     9781 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     9841 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9901 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9961 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
    10021 t
//
