LOCUS       sequence-318406-       11448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma
            cell-state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Schmitt MJ, Company C, Dramaretska Y, Barozzi I, Gohrig A, Kertalli 
            S, Grossmann M, Naumann H, Sanchez-Bailon MP, Hulsman D, Glass R, 
            Squatrito M, Serresi M, Gargiulo G
  TITLE     Phenotypic Mapping of Pathologic Cross-Talk between Glioblastoma and
            Innate Immune Cells by Synthetic Genetic Tracing.
  JOURNAL   Cancer Discov. 2021 Mar;11(3):754-777. doi: 
            10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.
  PUBMED    33361384
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-20-0219"; journalName: "Cancer Discov"; date: 
            "2021-03"; volume: "11"; issue: "3"; pages: "754-777"
COMMENT     Sequence Label: FH1-MGT#1-mVenus_PGK-H2B-CFP
FEATURES             Location/Qualifiers
     source          1..11448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    849..867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    885..903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(968..970,971..1681)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    1895..2483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1948..1968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2366..2377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2486..2502)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2487..2503)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        2627..3137
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3056..3074
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3149..3526
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3539..3548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3545..3547,3548..3550,3551..4264)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     LTR             4655..4835
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4861..4878)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4867..5091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5137..5565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5224..5243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5434..5455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5574..5594)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5579..5908
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5759..5894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5821..5840
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5956..6003
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6022..6396
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6526..6647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6563..6582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6617..6636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6696..6712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6696..6712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6709..6731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6720..6736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6744..6750,6751..6768,6769..6774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6789..6810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6927..6944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7098..7686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7178..7197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7857..8648,8649..8717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8480..8499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8718..8822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8897..8916)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9088..9467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9469..9667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9622..9642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9685..9865
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9912..10037
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10530..10763
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10948..10992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11290..11407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taatgcatat ttatttttag gaccagaaag ttaaagtgaa ttggatttga tccattttct
       61 gaaaggctgg caagaattct tgacattgca caggaatttc catgtcagca tgttctcaca
      121 tgtatgatct aatttagaga ttattttggg gggcgggggt tgaggaaatg gcatgactca
      181 gagtttaaaa gccccaaatc ttagctgtgc ctgtgtagct ttaccacata acccattgat
      241 aacttagttg tgcaaccatc accaccatct gttttcagaa ctcttttcat tttgcgaaac
      301 tgaaacccgt taagcactga tttcccactc tccctcctcc cagcccatag caaaccacca
      361 tcccaccagc actttcattt cgcaaatggc aaaactgaag ccgatattgt ggttgtgact
      421 tatcccaaag taatatacac ataaacctct atggatgagg aaaaagacag agggaaacta
      481 aaaattcaaa agaacaaatt tgactcacag atttgctgac tcatagttgt gacacttcct
      541 ggctcaggaa gttgaatttc attaagcctt tgtggtttgg ggctctgctg tgctttgaca
      601 gctctgatct cctcccttcc ggctgggctg tctggggcgc tctaaaatga gtgttgattt
      661 aatgcactgc cttcgcaccc gtgctggtgc gtcccgggga caggggtggc tgtgcggtgc
      721 cgcggcggcc ggcggggctc cttccccagc aggggtgggg acgctgagtc acggatctgt
      781 caccgctttg cacctctccg agccctcggg ggccaaagca aaagcgaaag cgatgtacac
      841 tagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
      901 agaacgtatg gagaccgaca ccctgctgct gtgggtgctg ctgctgtggg tgcccggcag
      961 caccggcgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1021 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1081 ctacggcaag ctgaccctga agctcatctg caccaccggc aagctgcccg tgccctggcc
     1141 caccctcgtg accaccctcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
     1201 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1261 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1321 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1381 gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa
     1441 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     1501 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1561 ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     1621 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctctacaa
     1681 gtccggactc agatctcgag ctgctgctgc agtcctggtg ctgttggtga ttgtgatcat
     1741 ctcacttatt gtcctggttg tcatttggtg aatctagata actgatcata atcagccata
     1801 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacgtacaa gtaaagcggc
     1861 cgcgactcta caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1921 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1981 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2041 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2101 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2161 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2221 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2281 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2341 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2401 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2461 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagga cctagaaaaa
     2521 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     2581 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtacgggg ttggggttgc
     2641 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     2701 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     2761 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     2821 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     2881 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     2941 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     3001 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     3061 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     3121 ccgacctctc tccccagggg gatctaccat gccagagcca gcgaagtctg ctcccgcccc
     3181 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
     3241 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
     3301 tgacaccggc atttcgtcca aggccatggg catcatgaac tcgtttgtga acgacatttt
     3361 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
     3421 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
     3481 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc
     3541 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3601 ggacggcgac gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac
     3661 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3721 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat
     3781 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgtaccat
     3841 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3901 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3961 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa
     4021 gaacggcatc aaggcccact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4081 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4141 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4201 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4261 gtaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4321 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4381 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     4441 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     4501 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     4561 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     4621 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     4681 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4741 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4801 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     4861 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4921 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4981 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5041 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5101 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5161 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5221 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5281 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5341 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5401 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5461 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5521 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5581 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5641 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5701 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5761 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5821 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5881 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5941 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6001 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     6061 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     6121 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     6181 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     6241 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     6301 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     6361 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     6421 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6481 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6541 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6601 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6661 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6901 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7501 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8161 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8461 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8821 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     8881 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8941 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9001 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9061 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     9121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     9181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     9241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     9301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     9361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     9421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     9481 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     9541 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     9601 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     9661 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     9721 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9781 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9841 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     9901 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     9961 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10021 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10081 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10141 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10201 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    10261 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    10321 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    10381 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    10441 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    10501 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    10561 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    10621 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    10681 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    10741 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    10801 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    10861 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    10921 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    10981 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    11041 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    11101 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    11161 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    11221 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
    11281 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    11341 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    11401 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaat
//
