LOCUS       sequence-318423-       10871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of mStrawberry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Yamamoto K, Venida A, Yano J, Biancur DE, Kakiuchi M, Gupta S, Sohn 
            ASW, Mukhopadhyay S, Lin EY, Parker SJ, Banh RS, Paulo JA, Wen KW, 
            Debnath J, Kim GE, Mancias JD, Fearon DT, Perera RM, Kimmelman AC
  TITLE     Autophagy promotes immune evasion of pancreatic cancer by degrading 
            MHC-I.
  JOURNAL   Nature. 2020 May;581(7806):100-105. doi: 10.1038/s41586-020-2229-5. 
            Epub 2020 Apr 22.
  PUBMED    32376951
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2229-5"; journalName: "Nature"; date: "2020-05";
            volume: "581"; issue: "7806"; pages: "100-105"
COMMENT     Sequence Label: pINDUCER20_mStrawberry
FEATURES             Location/Qualifiers
     source          1..10871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             28..661
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    708..833
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1330..1563
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1748..1792
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2085..2202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2257..2275,2276..2298,2299..2317,2318..2340,2341..2359,
                     2360..2382,2383..2401,2402..2424,2425..2443,2444..2466,
                     2467..2485,2486..2508,2509..2527)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2560..2598
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2595..2619
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2728..2752
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2758..3468
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2903..2921)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3171..3190
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3470..3494)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3547..3573
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3659..4870
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4707..4726
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4929..5546,5547..5552,5553..5588,5589..5591,5592..5627,
                     5628..5630,5631..5666,5667..5675)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5728..6301
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5895..5912)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6122..6141
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6317..7111
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6317..7111
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6371..6390)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6981..7000
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7288..7876
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7341..7361)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7759..7770)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7951..8184
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8303..8321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8389..8493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8494..8562,8563..9354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8712..8731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9525..10113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10014..10033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10267..10284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10524..10545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10560..10565,10566..10583,10584..10590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10598..10614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10603..10625
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10622..10638
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10622..10638
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10688..10707
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
       61 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
      121 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
      181 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
      241 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
      301 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
      361 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
      421 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
      481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      661 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      721 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      781 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      841 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      901 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      961 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1021 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1081 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1141 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     1201 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1261 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1321 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1381 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1441 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1501 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1561 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1621 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1681 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1741 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1801 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1861 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1921 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     1981 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2041 tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2101 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2161 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2221 agcagagatc cagtttggac taggatcctt taccactccc tatcagtgat agagaaaagt
     2281 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2341 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag
     2581 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2641 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaactagtc
     2701 cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagcagg cttcaccatg
     2761 gtgagcaagg gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2821 cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2881 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     2941 gcctgggaca tcctaacccc caacttcacc tacggctcca aggcctacgt gaagcacccc
     3001 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     3061 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     3121 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3181 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3241 ctgaagggcg agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3301 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcggc
     3361 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaact gtacgaacgc
     3421 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaaa cccagctttc
     3481 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     3541 ttcgaatacc catacgacgt cccagactac gcttagtaat gattaattaa actagaaatt
     3601 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gtaacccacc caagatctgg
     3661 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     3721 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     3781 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     3841 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     3901 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     3961 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     4021 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     4081 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     4141 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     4201 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     4261 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     4321 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     4381 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     4441 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     4501 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     4561 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     4621 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     4681 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     4741 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     4801 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     4861 tttgttagac gaagcttggt accgagctcg gatctccacc ccgtaccggt cctgcagtcg
     4921 aattcaccat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     4981 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     5041 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     5101 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     5161 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     5221 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     5281 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     5341 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     5401 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     5461 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     5521 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     5581 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     5641 ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatccg cggccgcact
     5701 agaggaattc cgcccctctc cccccccccc ctctccctcc ccccccccta acgttactgg
     5761 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     5821 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     5881 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     5941 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     6001 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     6061 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     6121 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     6181 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     6241 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     6301 agcttaccgg tccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6361 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6421 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6481 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6541 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6601 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6661 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6721 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6781 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6841 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6901 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6961 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7021 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7081 cttctatcgc cttcttgacg agttcttctg atgtacaagt aaagcggccg cgactctaga
     7141 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7201 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgtttagtcc ctcccaattc
     7261 gatatcaagc ttatcgcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     7321 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7381 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7441 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7501 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7561 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7621 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7681 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7741 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7801 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7861 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     7921 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7981 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8041 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8101 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8161 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8221 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     8281 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8341 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8401 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8461 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8521 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8581 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8641 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8701 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     8761 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8821 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8881 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8941 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9001 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9061 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9121 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9181 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9241 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9301 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9361 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9421 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9481 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9541 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9601 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9661 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9721 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9781 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9841 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9901 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9961 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10021 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10081 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10141 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10201 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10261 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10321 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    10381 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10441 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    10501 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10561 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10621 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    10681 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10741 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10801 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10861 aattgtattt g
//
