LOCUS       sequence-318428-        3399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SETD7 (Y305F) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Wallace AK, Chanut N, Voigt CA
  TITLE     Silica Nanostructures Produced Using Diatom Peptides with Designed 
            Post-Translational Modifications
  JOURNAL   Advanced Functional Materials 2020
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Advanced 
            Functional Materials 2020"
COMMENT     Sequence Label: pAKW.111A-SETD7
FEATURES             Location/Qualifiers
     source          1..3399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     272..291
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    485..503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        541..590
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      1769..1840
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1856..1883
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2096..2113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2230..2775)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2310..2329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2864..3399,1..280))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(2864..3399,1..280))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
       61 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
      121 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
      181 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
      241 gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
      301 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaca
      361 tcagagattt tgagacaatc tgagacccca attattgaag gctccctaac ggggggcctt
      421 tttttgtttc tggtctccaa tccgcttaac gatcgttggc tgttttcagc aggacgcact
      481 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacag
      541 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa
      601 taattttgtt taaagcacta gacagatatt ccaacaatta gatggatagc gacgacgaga
      661 tggtggagga ggcggtggaa gggcacctgg acgatgacgg attaccgcac gggttctgca
      721 cagtcaccta ctcctccaca gacagatttg aggggaactt tgttcacgga gaaaagaacg
      781 gacgggggaa gttcttcttc tttgatggca gcaccctgga ggggtattat gtggatgatg
      841 ccttgcaggg ccagggagtt tacacttacg aagatggggg agttctccag ggcacgtatg
      901 tagacggaga gctgaacggt ccagcccagg aatatgacac agatgggaga ctgatcttca
      961 aggggcagta taaagataac attcgtcatg gagtgtgctg gatatattac ccagatggag
     1021 gaagccttgt aggagaagta aatgaagatg gggagatgac tggagagaag atagcctatg
     1081 tgtaccctga tgagaggacc gcactttatg ggaaatttat tgatggagag atgatagaag
     1141 gcaaactggc tacccttatg tccactgaag aagggaggcc tcactttgaa ctgatgcctg
     1201 gaaattcagt gtaccacttt gataagtcga cttcatcttg catttctacc aatgctcttc
     1261 ttccagatcc ttatgaatca gaaagggttt atgttgctga atctcttatt tccagtgctg
     1321 gagaaggact tttttcaaag gtagctgtgg gacctaatac tgttatgtct ttttataatg
     1381 gagttcgaat tacacaccaa gaggttgaca gcagggactg ggcccttaat gggaacaccc
     1441 tctcccttga tgaagaaacg gtcattgatg tgcctgagcc ctataaccac gtatccaagt
     1501 actgtgcctc cttgggacac aaggcaaatc actccttcac tccaaactgc atctttgata
     1561 tgtttgtcca cccccgtttt gggcccatca aatgcatccg caccctgaga gcagtggagg
     1621 ccgatgaaga gctcaccgtt gcctatggct atgaccacag cccccccggg aagagtgggc
     1681 ctgaagcccc tgagtggtac caggtggagc tgaaggcctt ccaggccacc cagcaaaagt
     1741 gataatacta gagtcaggca ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1801 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1861 accttcgggt gggcctttct gcgtttataa atgacgaaga gaccctacgc gccatcgaat
     1921 tgcttggggt ctctcgctac tcgctccact aggcagacct cagcgctagc ggagtgtata
     1981 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa
     2041 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
     2101 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
     2161 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
     2221 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
     2281 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
     2341 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     2401 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     2461 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
     2521 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
     2581 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
     2641 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
     2701 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
     2761 ccaaaacgat ctcaagaaga tcatcttatt aaggggtctg acgctcagtg gaacgaaaaa
     2821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accttagaaa aactcatcga
     2881 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     2941 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     3001 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     3061 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     3121 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     3181 caaaatcact cgcatcaacc aaaccgttat tcatccgtga ttgcgcctga gcgagacgaa
     3241 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     3301 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     3361 atgctgtttt cccggggatc gcagtggtga gtaaccatg
//
