LOCUS       sequence-318457-       13353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a
            EBFP...
FEATURES             Location/Qualifiers
     source          1..13353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3043..3052
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3049..3747
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3798..4022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5071..6253
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5302..6244
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6354..6363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6363..6428
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6435..6455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7052..7276
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8325..9507
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8556..9498
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9608..9617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9614..9616,9617..9619,9620..10330)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9659..9680)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10358..10414
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10427..10825
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10876..11100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(11959..12768)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12682..12701
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12760..12779
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(12927..13353,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13007..13026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatct gaattcgaga ccacaggtct cggatccctc tagagggtat
     3001 ataatggggg ccactagtct actaccagag ctcatcgcta gcgccaccat ggtgagcgag
     3061 ctgattaagg agaacatgca catgaagctg tatatggagg gcaccgtgaa caaccaccac
     3121 ttcaagtgta cctccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     3181 aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     3241 tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     3301 ttccccgagg gcttcacatg ggagagagtc accacatacg aagatggggg cgtgctgacc
     3361 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     3421 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc
     3481 accgaaaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag
     3541 ctcgtgggcg ggggccacct gatctgcaac ttgaaaacca catacagatc caagaaaccc
     3601 gctaagaacc tcaagatgcc cggcgtctac tatgtggaca ggagactgga aagaatcaag
     3661 gaggccgaca aagaaaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     3721 ctccctagca aactggggca cagatgagcg gccgctcgag tctagagggc ccgtttaaac
     3781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3961 cagcaagggg gaggattggg aagtcaatag caggcatgct ggggatgcgg tgggctctat
     4021 ggcttctgag gcaggtcgag ggtacatgcg ccttactcct tgtgagcgca tcgcgataag
     4081 ctctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     4141 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     4201 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4261 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     4321 tttaggctga aagagagatt tagaatgaca gaatcataga acggcctgcg tacggatggg
     4381 gctctagctc tagagcttga tatcgaattc ctgcagcccc gctagaggga cagccccccc
     4441 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     4501 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     4561 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     4621 ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt
     4681 agaatgacag aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc aacagacggg
     4741 agagggcaaa accgatgttg acggactaat cctgacaaga tctggggagg tcgctgagta
     4801 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     4861 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtga
     4921 gtaattcata caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact
     4981 cccactaacg tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt
     5041 catcactgag gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc
     5101 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     5161 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5221 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5281 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     5341 acgggttatg gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat
     5401 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     5461 agccccttcg cctcgtgctt gagttgaggc ctggcttggg cgctggggcc gccgcgtgcg
     5521 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     5581 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     5641 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     5701 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     5761 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     5821 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     5881 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     5941 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     6001 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     6061 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     6121 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     6181 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     6241 tcaggtgtcg tgagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     6301 tgttttgacc tccatagaag agaccgggac cgatccaaat tctcgaggct agcgccacca
     6361 tggattataa ggatcacgat ggcgactaca aggaccacga tattgactac aaagacgatg
     6421 acgataaaat ggctcctaag aaaaagcgca aagtcggtat ccatggcgtt cccgtcgacg
     6481 gtggactgca ggccccacca accgatgttt ctttaggtga tgagttacat ttggacggtg
     6541 aggacgtagc tatggcacat gcagatgcct tagacgattt tgacttggat atgttaggag
     6601 atggagattc accaggacct ggtttcacac cacacgattc agctccatac ggtgctttgg
     6661 acatggccga tttcgaattt gaacagatgt ttactgatgc cttgggtata gacgaatacg
     6721 gtggtggagc tagacccggg gagcgcccct tccagtgtcg catttgcatg cggaactttt
     6781 cgcgccagga caggcttgac aggcataccc gtactcatac cggtgaaaaa ccgtttcagt
     6841 gtcggatctg tatgcgaaat ttctcccaga aggagcactt ggcggggcat ctacgtacgc
     6901 acaccggcga gaagccattc caatgccgaa tatgcatgcg caacttcagt cgccgcgaca
     6961 acctgaaccg gcacctaaaa acccacctga gaggatctta agcggccgct cgagtctaga
     7021 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7081 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7141 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7201 ggggtggggc aggacagcaa gggggaggat tgggaagtca atagcaggca tgctggggat
     7261 gcggtgggct ctatggcttc tgaggcaggt cgaagaacta tttcctggct gttacgcgag
     7321 cgcatcgcga taagctctag agggacagcc cccccccaaa gcccccaggg atgtaattac
     7381 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     7441 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     7501 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     7561 tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca tagaacggcc
     7621 tgcgtacgga tggggctcta gctctagagc ttgatatcga attcctgcag ccccgctaga
     7681 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
     7741 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
     7801 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
     7861 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa agctttaggc
     7921 tgaaagagag atttagaatg acagaatcat agaacggcct gggcgcgcct ggtggcgaac
     7981 gatcaacaga cgggagagga ctaaatagta gttcagacgc cgttaagcgc aagatctggg
     8041 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     8101 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     8161 agatatacgc gtgagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg
     8221 accgtcgtta aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac
     8281 ccgcccgctc tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc
     8341 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     8401 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     8461 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     8521 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     8581 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg
     8641 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     8701 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg
     8761 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     8821 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     8881 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     8941 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     9001 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     9061 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     9121 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     9181 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     9241 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     9301 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     9361 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     9421 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     9481 ttttttcttc catttcaggt gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga
     9541 gacgccatcc acgctgtttt gacctccata gaagagaacg ggaccgatcc aaattctcga
     9601 ggctagcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     9661 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgaggggcg agggcgaggg
     9721 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     9781 gccctggccc accctcgtga ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc
     9841 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     9901 gcgcaccatc ttcttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga
     9961 gggcgacacc ctagtgaacc gcatcgagct gaagggcgtc gacttcaagg aggacggcaa
    10021 catcctgggg cacaagctgg agtacaactt caacagccac aacatctata tcatggccgt
    10081 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacgtgg aggacggcag
    10141 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
    10201 gcccgacagc cactacctga gcacccagtc cgtgctgagc aaagacccca acgagaagcg
    10261 cgatcacatg gtcctgctgg agttccgcac cgccgccggg atcactctcg gcatggacga
    10321 gctgtacaag gaattcggta gcaagcttgg tagtggagca actaactttt ccctgctcaa
    10381 acaagccggg gatgtagagg agaaccccgg ccccgccccc cttaagatgg ccaagccttt
    10441 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
    10501 ctctgaggac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
    10561 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
    10621 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
    10681 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
    10741 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
    10801 ctctggttat gtgtgggagg gctaagcggc cgctcgagtc tagagggccc gtttaaaccc
    10861 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    10921 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    10981 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    11041 gcaaggggga ggattgggaa gtcaatagca ggcatgctgg ggatgcggtg ggctctatgg
    11101 cttctgaggc aggtcgaaga gactgcccga cacatcttag tgagcgcatc gcgataagct
    11161 ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag
    11221 ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga
    11281 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct
    11341 gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt
    11401 taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgcgta cggatggggc
    11461 tctagctcta gagcttgata tcgaattcct gcagccccgc tagagggaca gccccccccc
    11521 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
    11581 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
    11641 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
    11701 gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag
    11761 aatgacagaa tcatagaacg gcctgggcgc gcctggtggc gaacgatcaa cagacgggag
    11821 aggttacgag gatgcacatg tgaccgaggg atggaacgac gcgtaactca cgttaaggga
    11881 ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa
    11941 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
    12001 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
    12061 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
    12121 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
    12181 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
    12241 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
    12301 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
    12361 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
    12421 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg ggatcgcagt
    12481 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
    12541 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
    12601 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaagc gatagattgt
    12661 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
    12721 gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa caccccttgt
    12781 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
    12841 aatgtaacat cagagatttt gagacacggg ccatgctagc tccagcaaaa ggccaggaac
    12901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    12961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    13021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    13081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    13141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    13201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    13261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    13321 gctacagagt tcttgaagtg gtggcctaac tac
//
