LOCUS       sequence-318464-        4632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for C-terminal BirA* tagging of proteins in Saccharomyces 
            cerevisiae; Selection marker: TRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Salvatori R, Kehrein K, Singh AP, Aftab W, Moller-Hergt BV, Forne I,
            Imhof A, Ott M
  TITLE     Molecular Wiring of a Mitochondrial Translational Feedback Loop.
  JOURNAL   Mol Cell. 2020 Feb 20;77(4):887-900.e5. doi: 
            10.1016/j.molcel.2019.11.019. Epub 2019 Dec 26.
  PUBMED    31883951
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.11.019"; journalName: "Mol Cell"; date: 
            "2020-02-20- 20"; volume: "77"; issue: "4"; pages: "887-900.e5"
COMMENT     Sequence Label: pYM29-BirA*
FEATURES             Location/Qualifiers
     source          1..4632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             60..83
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1122..1148
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2286..2305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2287..2305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2392..2409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2563..3151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2643..2662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3322..4113,4114..4182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3945..3964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4183..4287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4355..4373
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4411..4433)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4533..4552
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaggtga cactatagaa cgcggccgcc agctgaagct tcgtacgctg caggtcgacg
       61 attacaagga cgacgacgac aagacgcgtg gtggtggtgg ttctggtggt ggtggttcta
      121 tgaaggacaa caccgtgccc ctgaagctga tcgccctgct ggctaacggc gagttccact
      181 ctggcgagca gctgggcgag acactgggca tgtctagagc cgccatcaac aagcacatcc
      241 agaccctgag agactggggc gtggacgtgt tcaccgtgcc tggcaagggc tacagcctgc
      301 ccgaacctat ccagctgctg aacgccaagc agatcctggg ccagctggac ggcggatctg
      361 tggctgtgct gcctgtgatc gacagcacca accagtacct gctggacaga atcggcgagc
      421 tgaagtccgg cgacgcctgt atcgctgagt accagcaggc tggcagaggc ggcaggggca
      481 gaaagtggtt ctctcccttc ggcgctaacc tgtacctgag catgttctgg cggctggaac
      541 agggacctgc cgctgctatc ggactgagcc tcgtgatcgg catcgtgatg gccgaggtgc
      601 tgagaaagct gggcgccgac aaagtgcgcg tgaagtggcc caacgacctg tatctgcagg
      661 accggaagct ggccggcatc ctggtggaac tgacaggcaa gacaggcgac gccgcccaga
      721 tcgtgattgg cgctggcatc aacatggcta tgagaagagt ggaagagtcc gtcgtgaacc
      781 agggctggat caccctgcag gaagccggca tcaacctgga ccggaacaca ctggccgcca
      841 tgctgatcag agagctgaga gccgccctgg aactgttcga gcaggaaggc ctggccccct
      901 acctgtccag atgggagaag ctggacaact tcatcaacag acccgtgaaa ctgatcatcg
      961 gcgacaaaga aatcctgggc atcagcagag gcatcgacaa gcagggcgct ctgctgctgg
     1021 aacaggacgg catcatcaag ccctggatgg gcggcgagat cagcctgaga tctgctgaga
     1081 agggcggcag cggacctggc ggaggcgctc cttaaggatc ctatccatat gacgttccag
     1141 attacgcttc tagctaaaaa gtggaacgat cattcactat atatatatca atttatatat
     1201 acgtatgtgt aattgaagaa agatacgttt tttcctctat tgagaggcct gctggatgaa
     1261 tagctttacc ttttctaaat ccttgatacc atcagtctct actcctccac ttacatcgac
     1321 accgatcgca tttggtaaca tattaatggc aacagaaacg ttatcaggat tcaatccacc
     1381 agcgataatg aattttatct cgggatgact tgcagaccaa ctggaaattg cactccaatt
     1441 caatttctca ccagtgccac cttcaccaga atcgaacaac gtcagcacat tgtctacgtg
     1501 ttcatacagg tccagtagta attcacaatc ctgtgggaac tggaacctct taatgattgg
     1561 aattgaagat gggatcaaag atctgtattc tttaatatct tcatctccat gtaattgtat
     1621 cacatctaga ttatattcgt ggtacagttg aaggacatca tcaacggact gatttctaaa
     1681 caccccgact agtttagtac ctttcacgtt ctcttgttgg tgaactgcag ttgaaatacc
     1741 tttcgcaaca gatgagtcaa tggttctttt cctaccggga acacaaatga tacctaagta
     1801 atcagcacca tcatccacag cagtctttgc agcttcaacg gtttgcaaac cacacacttt
     1861 aacgagcatc acagtagatt tcgcaagagg gtagctcgct cagagtaccc aagtaaatga
     1921 ttagtaaact gatgtttgat agttcaattt ttcaatgaaa taaccttata ttaaaattga
     1981 tattactatt atacaaaaat aaagaataaa ggatttgagt ttatacataa aataccatta
     2041 ttatttgttc agtgagagat accggggtat atgggatgtg tgtagtgata ccatgcaatc
     2101 atgtatcaaa catggaagaa gtgaaggggg agaaagaaac ttgaaaaaaa gaaaaccatt
     2161 tgaaaagagg aaaagtatct aattaagatc tttcacctct tagtcttctg gcctcgaggc
     2221 gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc
     2281 ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat
     2341 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3001 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4321 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4381 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4441 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     4501 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     4561 gcaccatatg gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat
     4621 catacacata cg
//
