LOCUS       sequence-318467-        9287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing iaaM for converting L-Trp to IAM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB
  TITLE     Synthetic mammalian signaling circuits for robust cell population 
            control.
  JOURNAL   Cell. 2022 Mar 17;185(6):967-979.e12. doi: 
            10.1016/j.cell.2022.01.026. Epub 2022 Mar 1.
  PUBMED    35235768
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.01.026"; journalName: "Cell"; date: 
            "2022-03-17- 17"; volume: "185"; issue: "6"; pages: "967-979.e12"
COMMENT     Sequence Label: vYM140_PB-pEFa1-iaaM(P.s)-Hygro
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2093..2114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2305..2324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2538..2560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2552..2569
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2553..2569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2699..2761
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        3204..4382
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3435..4373
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4330..4350
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(6145..6162)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6151..6358
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6391..6411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6396..6725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6576..6711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6638..6657
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6774..7799
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6774..7799
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(7859..7879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7922..8043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7959..7978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8013..8032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8566..8600
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9091..9107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9091..9107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9104..9126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9115..9131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9139..9145,9146..9163,9164..9169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9184..9205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagatc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2401 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2461 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2521 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc
     2581 gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta
     2641 cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc tagattaacc
     2701 ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa attgacgcat
     2761 gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta
     2821 ttatatttac acttacatac taataataaa ttcaacaaac aatttattta tgtttattta
     2881 tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatgagg
     2941 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
     3001 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
     3061 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
     3121 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaagg cctccacggc
     3181 cagcacctgc gtgacgtacg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3241 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3301 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3361 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3421 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3481 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     3541 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     3601 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     3661 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     3721 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     3781 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3841 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3901 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3961 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     4021 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     4081 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     4141 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     4201 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     4261 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     4321 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     4381 gaaccttcac gcaggtggaa ttctaactat aacggtccta aggtagcgaa ggtctgcgta
     4441 gccaccatgt acgaccattt taatagccca agcattgata tcctttacga ctacggccca
     4501 tttcttaaga agtgtgaaat gaccggcgga attgggtctt acagtgctgg cacaccaacc
     4561 ccacgtgttg ccattgtcgg agcagggatc tcagggctgg tggcagccac agaattgctt
     4621 cgcgccggcg tcaaggacgt cgttttgtac gagagccgcg acagaatcgg gggtcgggtg
     4681 tggtcccagg tgttcgatca gactcgacct cgctatattg ctgagatggg tgccatgcga
     4741 ttccccccct cggcgaccgg actcttccac tacctaaaga aatttggtat ctcaacatct
     4801 acaacttttc ctgatccagg tgttgtggat actgagctgc attaccgggg caaacgatat
     4861 cactggcctg ccggaaagaa acctccggag ctctttaggc gggtgtatga gggatggcag
     4921 tcgctgcttt ccgagggtta ccttttagaa ggtggatcac tggtcgcccc tcttgatatt
     4981 accgccatgt tgaagagcgg gagattagag gaagccgcca tcgcttggca gggctggcta
     5041 aacgtattcc gtgactgttc tttttataat gctatcgtgt gcatattcac aggtcgacac
     5101 cctccagggg gggaccgctg ggccaggcct gaagatttcg agctgtttgg ttccctgggg
     5161 ataggaagtg gcggctttct gcccgtattc caggccggct ttacggagat cttgcgcatg
     5221 gtgataaatg gctaccaatc cgaccagagg atgatcccag atggcatcag cagcttagct
     5281 gcgcgcctag ctgaccagag tttcgacggt aaggctctca gggaccgtgt ttgttttagc
     5341 agagtgggtc ggatttcccg ggaggccgaa aaaatcatta tacagaccga agccggcgaa
     5401 cagagggttt tcgaccgggt tatcgtgacc tcttcaaacc gagcaatgca aatgatccac
     5461 tgcttgaccg acagcgagtc ttttctcagt agggacgtgg ctagagcagt gagagaaaca
     5521 cacctaactg gctctagcaa gctctttatt ctgaccagaa ctaaattctg gatcaaaaac
     5581 aagctgccga ctacaattca gtctgacggg ctcgtacgcg gagtctattg ccttgattat
     5641 cagcccgatg agcctgaagg acatggcgtg gtcttgttgt catacacctg ggaggatgat
     5701 gctcagaaga tgctggctat gcccgacaag aagacaaggt gccaggtact ggtcgatgat
     5761 ctcgcagcga tccatccaac cttcgcctca tacctgctgc ccgtggatgg agattatgag
     5821 cggtatgtcc tgcaccacga ttggctgact gacccccata gtgcaggtgc tttcaaactg
     5881 aattatcccg gcgaggacgt gtattctcaa agactgttct tccagcccat gaccgccaac
     5941 tcccctaata aagacacagg gctctacctg gcaggctgct cttgtagttt tgcaggaggg
     6001 tggatcgaag gcgcagtgca aactgcactg aactccgctt gcgccgtgct ccgttccacg
     6061 ggcggacaac tgtccaaggg aaacccactc gactgtatta acgctagtta tcgatactaa
     6121 acctcattac cctgttatcc ctagcctcga ctgtgccttc tagttgccag ccatctgttg
     6181 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6241 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6301 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggacg
     6361 cggccgcaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6541 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6661 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6721 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa
     6781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     6841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     6901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     6961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7021 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     7081 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     7141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     7201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     7261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     7321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     7381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     7441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     7501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     7561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     7621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     7681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     7741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagg
     7801 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7861 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat ccctagaatt
     7921 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8041 ttaggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag
     8101 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     8161 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     8221 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     8281 gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     8341 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     8401 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     8461 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     8521 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     8581 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     8641 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     8701 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     8761 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     8821 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     8881 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     8941 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     9001 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     9061 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9121 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9181 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9241 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcgggga
//
